Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems

A - Papers appearing in refereed journals

Wan, Y., Gritsch, C., Tryfona, T., Ray, M. J., Andongabo, A. E., Hassani-Pak, K., Jones, H. D., Dupree, P., Karp, A., Shewry, P. R. and Mitchell, R. A. C. 2014. Secondary cell wall composition and candidate gene expression in developing willow (Salix purpurea) stems. Planta. 239, pp. 1041-1053.

AuthorsWan, Y., Gritsch, C., Tryfona, T., Ray, M. J., Andongabo, A. E., Hassani-Pak, K., Jones, H. D., Dupree, P., Karp, A., Shewry, P. R. and Mitchell, R. A. C.
Abstract

The properties of the secondary cell wall (SCW) in willow largely determine the suitability of willow biomass feedstock for potential bioenergy and biofuel applications. SCW development has been little studied in willow and it is not known how willow compares with model species, particularly the closely related genus Populus. To address this and relate SCW synthesis to candidate genes in willow, a tractable bud culture-derived system was developed in Salix purpurea, and cell wall composition and RNA-Seq transcriptome were followed in stems during early development. A large increase in SCW deposition in the period 0–2 weeks after transfer to soil was characterised by a big increase in xylan content, but no change in the frequency of substitution of xylan with glucuronic acid, and increased abundance of putative transcripts for synthesis of SCW cellulose, xylan and lignin. Histochemical staining and immunolabeling revealed that increased deposition of lignin and xylan was associated with xylem, xylem fibre cells and phloem fibre cells. Transcripts orthologous to those encoding xylan synthase components IRX9 and IRX10 and xylan glucuronyl transferase GUX1 in Arabidopsis were co-expressed, and showed the same spatial pattern of expression revealed by in situ hybridisation at four developmental stages, with abundant expression in proto-xylem, xylem fibre and ray parenchyma cells and some expression in phloem fibre cells. The results show a close similarity with SCW development in Populus species, but also give novel information on the relationship between spatial and temporal variation in xylan-related transcripts and xylan composition.

KeywordsHemicellulose; LM10 antibody; Secondary xylem; Tree stem transcriptome
Year of Publication2014
JournalPlanta
Journal citation239, pp. 1041-1053
Digital Object Identifier (DOI)doi:10.1007/s00425-014-2034-1
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCropping Carbon (CC) [ISPG]
Designing cereal seeds for nutrition and health
Design Cereal Seeds [Shewry/Mitchell]
The BBSRC Sustainable Bioenergy Centre (BSBEC): Perennial Bioenergy Crops Programme [2009-2014]
The BBSRC Sustainable Bioenergy Centre (BSBEC): Perennial Bioenergy Crops Programme: PSC component
Publisher's version
Output statusPublished
Publication dates
Online07 Feb 2014
Publication process dates
Accepted21 Jan 2014
Copyright licenseCC BY
PublisherSpringer
ISSN0032-0935

Permalink - https://repository.rothamsted.ac.uk/item/8qy14/secondary-cell-wall-composition-and-candidate-gene-expression-in-developing-willow-salix-purpurea-stems

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