Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences

A - Papers appearing in refereed journals

Blunden, R., Wilkes, T. J., Forster, J. W., Jimenez, M. M., Sandery, M. J., Karp, A. and Jones, R. N. 1993. Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences. Genome. 36 (4), pp. 706-711.

AuthorsBlunden, R., Wilkes, T. J., Forster, J. W., Jimenez, M. M., Sandery, M. J., Karp, A. and Jones, R. N.
Abstract

A second family of highly repeated sequences has been identified on the B chromosome of rye (Secale cereale). The E3900 family was detected as a variant band in EcoRI digests of +B DNA. A clone of the basic repeat of the family was obtained, and the organization of the family was investigated by genomic hybridization. The E3900 family has no apparent homology to the A chromosome sequences of rye or other members of the Gramineae. The family has been localized by in situ hybridization to the end of the long arm of the rye B chromosome. The previously characterized E1100 sequence shows in situ hybridization to the same location as the E3900 family. These results are discussed in light of current theories of the origin of B chromosomes.

Keywordschromosome-b; secale-cereale; repeated sequence; Cloning; in-situ; Hybridization; Dna; Wheat
Year of Publication1993
JournalGenome
Journal citation36 (4), pp. 706-711
Digital Object Identifier (DOI)doi:10.1139/g93-095
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN08312796

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