Microsatellite markers for diverse Salix species

A - Papers appearing in refereed journals

Barker, J. H. A., Pahlich, A., Trybush, S., Edwards, K. J. and Karp, A. 2003. Microsatellite markers for diverse Salix species. Molecular Ecology Notes. 3 (1), pp. 4-6. https://doi.org/10.1046/j.1471-8286.2003.00332.x

AuthorsBarker, J. H. A., Pahlich, A., Trybush, S., Edwards, K. J. and Karp, A.

Forty-six microsatellites were isolated from an enriched library of Salix butjatica and tested on 20 individuals (of nine species/hybrids) from the National Willows Collection (IACR-Long Ashton Research Station, UK). Twenty-nine were monomorphic, gave multilocus or unscorable patterns, or were duplicates. The remaining 17 microsatellites gave 2-22 alleles/ locus. Three microsatellites successfully cross-amplified in 31 additional Salix species. A further six were tested on panels comprising 6-25 individuals from the 31 species. Cross-amplification was successful in all cases. These results suggest that the microsatellites isolated here should prove useful for population studies in a wide range of Salix species.

KeywordsBiochemistry & Molecular Biology; Ecology; Evolutionary Biology
Year of Publication2003
JournalMolecular Ecology Notes
Journal citation3 (1), pp. 4-6
Digital Object Identifier (DOI)https://doi.org/10.1046/j.1471-8286.2003.00332.x
Open accessPublished as non-open access
Funder project or code431
Project: 3860

Permalink - https://repository.rothamsted.ac.uk/item/891w9/microsatellite-markers-for-diverse-salix-species

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