Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability

A - Papers appearing in refereed journals

Palomo-Rios, E., Macalpine, W. J., Shield, I. F., Amey, J. S., Karaoglu, C., West, J., Hanley, S. J., Krygier, R., Karp, A. and Jones, H. D. 2015. Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability. Canadian Journal Of Forest Research. 45 (11), pp. 1662-1667.

AuthorsPalomo-Rios, E., Macalpine, W. J., Shield, I. F., Amey, J. S., Karaoglu, C., West, J., Hanley, S. J., Krygier, R., Karp, A. and Jones, H. D.
Abstract

Willow is a versatile crop with considerable potential as a source of renewable biomass for bioenergy. Although breeding new varieties takes less time compared with some other tree species, producing new willow varieties is still a slow, labour-intensive process, partly because clonally propagating the results of each cross is a bottleneck early in the breeding scheme. In this paper, we describe a facile, rapid method for the in vitro culture of a wide range of willow genotypes. We have developed a combination of media and methods for efficient tissue-culture propagation to rapidly multiply individual plants and simultaneously produce clean, stock germplasm applicable to a wide range of willow genotypes that can be phytosanitary tested to demonstrate their disease-free status. The micropropagation method described could generate in the order of 5000 viable, transplantable clones from a single plant in just 24 weeks and was used to produce phytosanitary tested breeding material for export to overcome restriction on the international transport of woody cuttings. This method could represent a valuable biotechnology adjunct to willow breeding programmes and could accommodate early selection via molecular or biochemical markers.

Keywords
Year of Publication2015
JournalCanadian Journal Of Forest Research
Journal citation45 (11), pp. 1662-1667
Digital Object Identifier (DOI)doi:10.1139/cjfr-2015-0055
Open accessPublished as green open access
Funder project or codeWheat
Cropping Carbon (CC) [ISPG]
Bioenergy value chains: Whole systems analysis and optimisation
Maximising carbon harvest from perennial crops
The BBSRC Sustainable Bioenergy Centre (BSBEC): Perennial Bioenergy Crops Programme
Development and application of cereal transformation technologies
Wheat biotechnology and gene validation platform
Integrating carbon systems
[20:20 Wheat] Maximising yield potential of wheat
FunderBiotechnology and Biological Sciences Research Council
Accepted author manuscriptcjfr-2015-0055.pdf
Output statusPublished
Publication dates
Online24 Sep 2015
Publication process dates
Accepted22 Jun 2015
Copyright licenseCC BY-NC-ND
PublisherCanadian Science Publishing, NRC Research Press
ISSN0045-5067

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