Analysis of rye B-chromosome structure using fluorescence in situ hybridization (FISH)

A - Papers appearing in refereed journals

Wilkes, T. M., Francki, M. G., Langridge, P., Karp, A., Jones, R. N. and Forster, J. W. 1995. Analysis of rye B-chromosome structure using fluorescence in situ hybridization (FISH). Chromosome Research. 3 (8), pp. 466-472. https://doi.org/10.1007/bf00713960

AuthorsWilkes, T. M., Francki, M. G., Langridge, P., Karp, A., Jones, R. N. and Forster, J. W.
Abstract

Fluorescence in situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomic in situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A-B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.

Keywordsb chromosome; DNA sequence composition; fluorescence in situ; Hybridization; repetitive sequence; Secale Cereale; Translocations; repeated DNA-sequences; ribosomal-rna genes; secale-cereale; insitu; Wheat; Heterochromatin; Identification; Cloning; family; origin
Year of Publication1995
JournalChromosome Research
Journal citation3 (8), pp. 466-472
Digital Object Identifier (DOI)https://doi.org/10.1007/bf00713960
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN09673849

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