Analysis of rye B-chromosome structure using fluorescence in situ hybridization (FISH)

A - Papers appearing in refereed journals

Wilkes, T. M., Francki, M. G., Langridge, P., Karp, A., Jones, R. N. and Forster, J. W. 1995. Analysis of rye B-chromosome structure using fluorescence in situ hybridization (FISH). Chromosome Research. 3 (8), pp. 466-472.

AuthorsWilkes, T. M., Francki, M. G., Langridge, P., Karp, A., Jones, R. N. and Forster, J. W.
Abstract

Fluorescence in situ hybridization (FISH) has been used to analyse the structure of the rye B chromosome. Genomic in situ hybridization (GISH) demonstrates the high level of overall similarity between A and B chromosomes of rye, as well as the presence of a number of specific sequences. The B-specific repeat families D1100 and E3900 have been analysed in terms of their physical location and possible contiguity. Rye Bs contain members of the rye-specific dispersed repetitive family R173, as well as centromeric regions similar to those of the As. The B chromosomes analysed in our study lack detectable rDNA sequences. Anomalous results have been obtained with a number of subtelomeric repetitive probes from rye. Bs usually lack these sequences, but evidence is presented that in some cases A-B translocation events may relocate such sequences from the As to the Bs. These data are discussed in the context of current models for the origin of the B chromosome.

Keywordsb chromosome; DNA sequence composition; fluorescence in situ; Hybridization; repetitive sequence; Secale Cereale; Translocations; repeated DNA-sequences; ribosomal-rna genes; secale-cereale; insitu; Wheat; Heterochromatin; Identification; Cloning; family; origin
Year of Publication1995
JournalChromosome Research
Journal citation3 (8), pp. 466-472
Digital Object Identifier (DOI)doi:10.1007/bf00713960
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN09673849

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