Shoot formation from explant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants

A - Papers appearing in refereed journals

Wheeler, V. A., Evans, N. E., Foulger, D., Webb, K. J., Karp, A., Franklin, J. and Bright, S. W. J. 1985. Shoot formation from explant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants. Annals of Botany. 55 (3), pp. 309-320.

AuthorsWheeler, V. A., Evans, N. E., Foulger, D., Webb, K. J., Karp, A., Franklin, J. and Bright, S. W. J.
Abstract

Plants were regenerated in quantity from cultured excised stem, rachis or leaf pieces of 13 potato (Solarium tuberosum L.) cultivars using modifications of a two-step procedure. Pronounced differences were observed in the response of different cultivars and explant sources to medium formulations containing different auxins or cytokinins. A few plants were recovered from tuber pieces of seven cultivars by a different two-step procedure. Morphological differences from control plants were observed in some of the primary regenerants. Anthocyanin pigmentation of tubers was scored and stable changes to red-splashed white or white tubers were found in some plants regenerated from cv. Desiree. Regenerants from the particoloured cultivars Cara and King Edward often lost this trait but it returned to varying extents in subsequent generations. Most of the regenerated plants (87 per cent) contained the euploid number of chromosomes (2n = 4x = 48). The methods are suitable for production of plants for assessment of somaclonal variation in a range of genotypes.

KeywordsPotato breeding; Somaclonal Variation; Chromosome variation; skin colour; explant culture; plant regeneration,; solanum tuberosum L. potato
Year of Publication1985
JournalAnnals of Botany
Journal citation55 (3), pp. 309-320
Digital Object Identifier (DOI)doi:10.1093/oxfordjournals.aob.a086907
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Output statusPublished
Publication dates
Print01 Mar 1985
Publication process dates
Accepted18 Sep 1984
Copyright licensePublisher copyright
ISSN0305-7364
PublisherOxford University Press (OUP)

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