Analysis of cereal chromosomes by atomic force microscopy

A - Papers appearing in refereed journals

Mcmaster, T. J., Winfield, M. O., Karp, A. and Miles, M. J. 1996. Analysis of cereal chromosomes by atomic force microscopy. Genome. 39 (2), pp. 439-444.

AuthorsMcmaster, T. J., Winfield, M. O., Karp, A. and Miles, M. J.
Abstract

Atomic force microscopy has been applied to the study of plant chromosomes from cereal grasses Triticum aestivum (bread wheat), Triticum tauschii, and Hordeum vulgare (barley). Using standard mitotic metaphase squashes, high resolution images have been obtained of untreated chromosomes and also of chromosomes after C-banding, N-banding, and in situ hybridization. The true 3-dimensional nature of the images permits detailed analysis of the surface structure and, on untreated uncoated chromosomes, surface features on a length scale consistent with nucleosome structures have been observed. C+ and N+ regions are manifest as areas of high relief on a slightly collapsed chromosome structure. In situ hybridization leads to a more severe degradation of the native structure, although it is still possible to correlate the optical signal with the topography of the hybridized chromosome.

Keywordsatomic force microscope; afm; Chromosomes; c-banding; In Situ; Hybridization; scanning electron-microscopy; plant chromosomes; Resolution; surface; bands; Wheat
Year of Publication1996
JournalGenome
Journal citation39 (2), pp. 439-444
Digital Object Identifier (DOI)doi:10.1139/g96-055
Open accessPublished as non-open access
Output statusPublished
ISSN0831-2796
PublisherCanadian Science Publishing, NRC Research Press

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