Cytological and molecular evidence of deletion of ribosomal RNA genes in chromosome 6 of barley (Hordeum vulgare)

A - Papers appearing in refereed journals

Gecheff, K., Hvarleva, T., Georgiev, S., Wilkes, T. and Karp, A. 1994. Cytological and molecular evidence of deletion of ribosomal RNA genes in chromosome 6 of barley (Hordeum vulgare). Genome. 37 (3), pp. 419-425.

AuthorsGecheff, K., Hvarleva, T., Georgiev, S., Wilkes, T. and Karp, A.
Abstract

The barley chromosomal mutant T-35, in which only one pair of satellite chromosomes is apparent, was analyzed using a range of cytological and molecular techniques. Using conventional Feulgen staining, Giemsa and silver banding, in situ hybridization, and Southern blot analysis, unequivocal cytological and molecular evidence was obtained that T-35 is a homozygous deletion of rRNA genes residing in the nucleolus organizer region (NOR) of chromosome 6. According to the criteria of arm ratio and Giemsa-banding pattern of this chromosome, the deletion involved the whole NOR, one of the breakpoints being localized in the short arm proximally to the NOR-associated heterochromatic band, the other probably in the satellite of the chromosome. As a result of this deletion, an increased activity of the rRNA genes (as indicated by the size of the silver bands) on the other NOR-bearing chromosome (chromosome 7) was observed. The possible reasons for this phenomenon are discussed.

KeywordsBarley; nucleolar organizing region; deletion; silver and giemsa; Banding; In Situ Hybridization; DNA-sequences; Wheat; Identification; locus; Maize
Year of Publication1994
JournalGenome
Journal citation37 (3), pp. 419-425
Digital Object Identifier (DOI)doi:10.1139/g94-059
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN08312796

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