Variation in telomeric heterochromatin in somaclones of rye

A - Papers appearing in refereed journals

Karp, A., Owen, P. G., Steele, S. H., Bebeli, P. J. and Kaltsikes, P. J. 1992. Variation in telomeric heterochromatin in somaclones of rye. Genome. 35 (4), pp. 590-593.

AuthorsKarp, A., Owen, P. G., Steele, S. H., Bebeli, P. J. and Kaltsikes, P. J.
Abstract

Somaclonal variation in telomeric heterochromatin was detected by in situ hybridization with the het 1 probe, which hybridizes to a 380-bp repeated sequence family. In a control cultivar, Gazelle, large blocks of signal were detected at the telomeres but not at the centromeres or the secondary constrictions. In the donor line, 7R - , labelling was restricted to small telomeric dots, confirming that the large telomeric blocks had been removed in selection of this line. In situ hybridization with the het 1 probe to chromosomes of selfed progeny from 50 plants regenerated from independent cultured immature embryos of the 7R - line revealed variant patterns for three regenerants. In the progeny of two regenerants, a new interstitial hybridization site was detected on the short arm of a submetacentric chromosome. This site was not at the nucleolus organizer. In the progeny of the third regenerant two changes were detected: an enlarged telomeric block on the long arm of an unidentified chromosome and an interstitial site on the long arm of chromosome 6. All three regenerated plants had shown normal morphology and meiotic behaviour. The identification of somaclonal variants in telomeric heterochromatin provides further evidence for variation in repeated DNA sequences in plant tissue culture.

Keywordsrye (secale-cereale); Somaclonal Variation; insitu hybridization; repeated sequence; Heterochromatin; telomere; repeated DNA-sequences; tissue-culture; callus-cultures; secale-cereale; nor loci; sativa-l; Chromosomes; Plants; Amplification; Wheat
Year of Publication1992
JournalGenome
Journal citation35 (4), pp. 590-593
Digital Object Identifier (DOI)doi:10.1139/g92-088
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
ISSN08312796

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