A - Papers appearing in refereed journals
Rivera, R., Edwards, K. J., Barker, J. H. A., Arnold, G. M., Ayad, G., Hodgkin, T. and Karp, A. 1999. Isolation and characterization of polymorphic microsatellites in Cocos nucifera L. Genome. 42 (4), pp. 668-675.
|Authors||Rivera, R., Edwards, K. J., Barker, J. H. A., Arnold, G. M., Ayad, G., Hodgkin, T. and Karp, A.|
Microsatellites or simple sequence repeats (SSRs) were isolated from coconut (Cocos nucifera) and tested for polymorphism on restricted germplasm. Sequencing of 197 clones from a cv. Tagnanan Tall-enriched genomic library showed that 75% contained a microsatellite, of which 64% were dinucleotide (GA/CT, CA/GT and GC/CG), 6% were trinucleotide, and 30% were compound repeats. Of 41 primer pairs tested on Tagnanan Tall genomic DNA, 38 gave the expected size product, two amplified two loci, and another gave a multilocus pattern. On 20 coconut samples, the 38 SSRs detected 198 alleles (average: 5.2 alleles per microsatellite). Genetic diversity (D = 1 - Sigma p(i)(2)) values ranged from 0.141 to 0.809. Heterozygotes were present at high frequencies among some dwarf samples. Analysis of similarity matrices based either on shared alleles at each locus (simple matching coefficient) or on allele bands across all loci (Jaccard coefficient) showed similar results. Dwarfs grouped separately from tails and showed less genetic diversity. In a wider test on 40 samples, 8 SSRs detected 64 alleles (average: eight alleles per microsatellite). These results indicate the high potential of microsatellites to detect genetic diversity in coconut germplasm.
|Keywords||Molecular Markers; microsatellite; SSR; Cocos Nucifera; coconut; simple sequence repeats; palm populations; Markers; Diversity; Plants; Identification; Genotypes; Abundance; Genetics; Maize|
|Year of Publication||1999|
|Journal citation||42 (4), pp. 668-675|
|Digital Object Identifier (DOI)||doi:10.1139/gen-42-4-668|
|Open access||Published as non-open access|
|Funder||Biotechnology and Biological Sciences Research Council|
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