Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle

A - Papers appearing in refereed journals

Lowe, A. J., Jones, A. E., Raybould, A. F., Trick, M., Moule, C. L. and Edwards, K. J. 2002. Transferability and genome specificity of a new set of microsatellite primers among Brassica species of the U triangle. Molecular Ecology Notes. 2 (1), pp. 7-11.

AuthorsLowe, A. J., Jones, A. E., Raybould, A. F., Trick, M., Moule, C. L. and Edwards, K. J.
Abstract

We present a new set of 12 highly polymorphic simple sequence repeat primer sequences for use with Brassica species. These new primers, and four from A.K.S. SzewcMcFadden and colleagues, were tested in four Brassica species (B. rapa, B. napus, B. oleracea and B. nigra). Most primers successfully amplified products within all species and were polymorphic. Due to the risk of gene flow from GM oilseed rape to its wild relatives, hybrid formation in the Brassicaceae is of great interest. We identify six primer pairs as specific to the A, B or C genomes that could be used to identify such hybrids.

KeywordsBiochemistry & Molecular Biology; Ecology; Evolutionary Biology
Year of Publication2002
JournalMolecular Ecology Notes
Journal citation2 (1), pp. 7-11
Digital Object Identifier (DOI)doi:10.1046/j.1471-8286.2002.00126.x
Open accessPublished as non-open access
Funder project or code411
999
ISSN14718278
PublisherWiley

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