QTL mapping of enzymatic saccharification in short rotation coppice willow and its independence from biomass yield

A - Papers appearing in refereed journals

Brereton, N. J. B., Pitre, F. E., Hanley, S. J., Ray, M. J., Karp, A. and Murphy, R. J. 2010. QTL mapping of enzymatic saccharification in short rotation coppice willow and its independence from biomass yield. BioEnergy Research. 3 (3), pp. 251-261.

AuthorsBrereton, N. J. B., Pitre, F. E., Hanley, S. J., Ray, M. J., Karp, A. and Murphy, R. J.
Abstract

Short rotation coppice (SRC) willows (Salix spp.) are fast-growing woody plants which can achieve high biomass yields over short growth cycles with low agrochemical inputs. Biomass from SRC willow is already used for heat and power, but its potential as a source of lignocellulose for liquid transport biofuels has still to be assessed. In bioethanol production from lignocellulose, enzymatic saccharification is used as an approach to release glucose from cellulose in the plant cell walls. In this study, 138 genotypes of a willow mapping population were used to examine variation in enzymatic glucose release from stem biomass to study relationships between this trait and biomass yield traits and to identify quantitative trait loci (QTL) associated with enzymatic saccharification yield. Significant natural variation was found in glucose yields from willow stem biomass. This trait was independent of biomass yield traits. Four enzyme-derived glucose QTL were mapped onto chromosomes V, X, XI, and XVI, indicating that enzymatic saccharification yields are under significant genetic influence. Our results show that SRC willow has strong potential as a source of bioethanol and that there may be opportunities to improve the breeding programs for willows for increasing enzymatic saccharification yields and biofuel production.

KeywordsBiofuel; Biomass; QTL; Enzymatic saccharification; Willow (Salix)
Year of Publication2010
JournalBioEnergy Research
Journal citation3 (3), pp. 251-261
Digital Object Identifier (DOI)doi:10.1007/s12155-010-9077-3
Open accessPublished as non-open access
Funder project or codeBCC
The BBSRC Sustainable Bioenergy Centre (BSBEC): Perennial Bioenergy Crops Programme [2009-2014]
FunderBiotechnology and Biological Sciences Research Council
Output statusPublished
Publication dates
Online27 Jan 2010
Copyright licensePublisher copyright
PublisherSpringer
ISSN1939-1234

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