Steve Hanley

Name	Steve Hanley
Job title	Senior Research Scientist - Head of Genomics
Email address	steve.hanley@rothamsted.ac.uk
Department	Plant Sciences and the Bioeconomy
Research cluster	CAS: Genomics and Bioinformatics
ORCID	https://orcid.org/0000-0002-3187-9260
Preferred citation	Hanley, S. J.
Office	Harpenden

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Research outputs

Breeding for novel chemistry in willow: new hetero Diels-Alder cyclodimers based on arbusculoidin and salicortin suggest parallel biosynthetic pathways

Noleto-Dias, C., Lomax, C., Bellisai, A., Ruvo, G., Harflett, C., Macalpine, W. J., Hanley, S. J., Beale, M. H. and Ward, J. L. 2024. Breeding for novel chemistry in willow: new hetero Diels-Alder cyclodimers based on arbusculoidin and salicortin suggest parallel biosynthetic pathways. Plants - Basel. 13 (12), p. 1609. https://doi.org/10.3390/plants13121609

Epidemiological studies of pan-azole resistant Aspergillus fumigatus populations sampled during tulip cultivation show clonal expansion with acquisition of multi-fungicide resistance as potential driver

Fraaije, B. A., Atkins, S. L., Santos, R. F., Hanley, S. J., West, J. S. and Lucas, J. 2021. Epidemiological studies of pan-azole resistant Aspergillus fumigatus populations sampled during tulip cultivation show clonal expansion with acquisition of multi-fungicide resistance as potential driver . Microorganisms. 9 (11), p. 2379. https://doi.org/10.3390/microorganisms9112379

Allele mining in diverse accessions of tropical grasses to improve forage quality and reduce environmental impact

Hanley, S. J., Pellny, T. K., De Vega, J. J., Castiblanco, V., Arango, J., Eastmond, P. J., Heslop-Harrison, J. S. and Mitchell, R. A. C. 2021. Allele mining in diverse accessions of tropical grasses to improve forage quality and reduce environmental impact. Annals of Botany. 128 (5), pp. 627-637. https://doi.org/10.1093/aob/mcab101

Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2

Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2 . Plant Biotechnology Journal. 19 (8), pp. 1602-1613. https://doi.org/10.1111/pbi.13573

The Multi-Fungicide Resistance Status of Aspergillus fumigatus Populations in Arable Soils and the Wider European Environment

Fraaije, B. A., Atkins, S. L., Hanley, S. J., Macdonald, A. J. and Lucas, J. 2020. The Multi-Fungicide Resistance Status of Aspergillus fumigatus Populations in Arable Soils and the Wider European Environment. Frontiers in Microbiology. 11, p. 599233. https://doi.org/10.3389/fmicb.2020.599233

A willow UDP-glycosyltransferase involved in salicinoid biosynthesis

Kulasekaran, S., Cerezo-Medina, S., Harflett, C., Lomax, C., DeJong, F., Rendour, A., Ruvo, G., Hanley, S. J., Beale, M. H. and Ward, J. L. 2020. A willow UDP-glycosyltransferase involved in salicinoid biosynthesis. Journal of Experimental Botany. 72 (5), p. 1634–1648. https://doi.org/10.1093/jxb/eraa562

Breeding progress and preparedness for mass-scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow, and poplar

Clifton-Brown, J., Harfouche, A., Casler, M. D., Jones, H. D., Macalpine, W. J., Murphy-Bokern, D., Smart, L. B., Adler, A., Ashman, C., Awty-Carroll, D., Bastien, C., Bopper, S., Botnari, V., Brancourt-Hulmel, M., Chen, Z., Clark, L. V., Cosentino, S., Dalton, S., Davey, C., Dolstra, O., Donnison, I., Flavell, R., Greef, J., Hanley, S. J., Hastings, A., Hertzberg, M., Hsu, T., Huang, L., Iurato, A., Jensen, E., Jin, X., Jørgensen, U., Kiesel, A., Kim, D., Liu, J., McCalmont, J. P., McMahon, B. G., Mos, M., Robson, P., Sacks, E. J., Sandu, A., Scalici, G., Schwarz, K., Scordia, D., Shafiei, R., Shield, I. F., Slavov, G., Stanton, B. J., Swaminathan, K., Taylor, G., Torres, A. F., Trindade, L. M., Tschaplinski, T., Tuskan, J., Yamada, T., Yu, C. Y., Zalesny, R., Zong, J. and Lewandowski, I. 2018. Breeding progress and preparedness for mass-scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow, and poplar. Global Change Biology. Bioenergy. https://doi.org/10.1111/gcbb.12566

Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability

Palomo-Rios, E., Macalpine, W. J., Shield, I. F., Amey, J. S., Karaoglu, C., West, J., Hanley, S. J., Krygier, R., Karp, A. and Jones, H. D. 2015. Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability. Canadian Journal Of Forest Research. 45 (11), pp. 1662-1667. https://doi.org/10.1139/cjfr-2015-0055

G-fibre cell wall development in willow stems during tension wood induction

Gritsch, C., Wan, Y., Mitchell, R. A. C., Shewry, P. R., Hanley, S. J. and Karp, A. 2015. G-fibre cell wall development in willow stems during tension wood induction. Journal of Experimental Botany. 66 (20), pp. 6447-6459. https://doi.org/10.1093/jxb/erv358

Characterisation of the willow phenylalanine ammonia-lyase (Pal) gene family reveals expression differences compared with poplar

De Jong, F., Hanley, S. J., Beale, M. H. and Karp, A. 2015. Characterisation of the willow phenylalanine ammonia-lyase (Pal) gene family reveals expression differences compared with poplar. Phytochemistry. 117 (September), pp. 90-97. https://doi.org/10.1016/j.phytochem.2015.06.005

Association mapping in Salix viminalis L. (Salicaceae) - identification of candidate genes associated with growth and phenology

Hallingbäck, H. R., Fogelqvist, J., Powers, S. J., Turrion-Gomez, J., Rossiter, R., Amey, J., Martin, T., Weih, M., Gyllenstrand, N., Karp, A., Lagercrantz, U., Hanley, S. J., Berlin, S. and Rönnberg-Wästljung, A-C. 2016. Association mapping in Salix viminalis L. (Salicaceae) - identification of candidate genes associated with growth and phenology. Global Change Biology. Bioenergy. 8 (3), pp. 670-685. https://doi.org/10.1111/gcbb.12280

Breeding willow for short rotation coppice energy cropping

Shield, I. F., Macalpine, W. J., Hanley, S. J. and Karp, A. 2015. Breeding willow for short rotation coppice energy cropping. in: Cruz, V. M. V. and Dierig, D. A. (ed.) Industrial crops. Handbook of Plant Breeding 9 Springer, New York. pp. 67-80

Genetic diversity, population structure and phenotypic variation in European Salix viminalis L. (Salicaceae)

Berlin, S., Trybush, S. O., Fogelqvist, J., Gyllenstrand, N., Hallingback, H. R., Ahman, I., Nordh, N-E., Shield, I. F., Powers, S. J., Weih, M., Lagercrantz, U., Ronnberg-Wastljung, A-C., Karp, A. and Hanley, S. J. 2014. Genetic diversity, population structure and phenotypic variation in European Salix viminalis L. (Salicaceae). Tree Genetics & Genomes. 10 (6), pp. 1595-1610. https://doi.org/10.1007/s11295-014-0782-5

Functional screening of willow alleles in Arabidopsis combined with QTL mapping in willow (Salix) identifies SxMAX4 as a coppicing response gene

Salmon, J., Ward, S. P., Hanley, S. J., Leyser, O. and Karp, A. 2014. Functional screening of willow alleles in Arabidopsis combined with QTL mapping in willow (Salix) identifies SxMAX4 as a coppicing response gene. Plant Biotechnology Journal. 12 (4), pp. 480-491. https://doi.org/10.1111/pbi.12154

Genetics, genomics and crop modelling: integrative approaches to the improvement of biomass willows

Karp, A., Richter, G. M., Shield, I. F. and Hanley, S. J. 2014. Genetics, genomics and crop modelling: integrative approaches to the improvement of biomass willows. in: McCann, M. C. (ed.) Plants and bioenergy. Advances in Plant Biology, Vol 4 Springer, New York. pp. 107-130

Genetic strategies for dissecting complex traits in biomass willows (Salix spp.)

Hanley, S. J. and Karp, A. 2014. Genetic strategies for dissecting complex traits in biomass willows (Salix spp.). Tree Physiology. 34 (11), pp. 1167-1180. https://doi.org/10.1093/treephys/tpt089

Insights into nitrogen allocation and recycling from nitrogen elemental analysis and 15N isotope labelling in 14 genotypes of willow

Brereton, N. J. B., Pitre, F. E., Shield, I. F., Hanley, S. J., Ray, M. J., Murphy, R. J. and Karp, A. 2014. Insights into nitrogen allocation and recycling from nitrogen elemental analysis and 15N isotope labelling in 14 genotypes of willow. Tree Physiology. 34 (11), pp. 1252-1262. https://doi.org/10.1093/treephys/tpt081

Using Arabidopsis to study shoot branching in biomass willow

Ward, S. P., Salmon, J., Hanley, S. J., Karp, A. and Leyser, O. 2013. Using Arabidopsis to study shoot branching in biomass willow. Plant Physiology. 162 (June), pp. 800-811. https://doi.org/10.1104/pp.113.218461

Near infrared spectroscopy (NIRS) for screening condensed tannins in a willow germplasm collection

Klongsiriwet, C., Karp, A., Hanley, S. J., Barnes, R., Falchero, L. and Mueller-Harvey, I. 2013. Near infrared spectroscopy (NIRS) for screening condensed tannins in a willow germplasm collection. Thieme. https://doi.org/10.1055/s-0033-1352230

A SNaPshot assay for the rapid and simple detection of known point mutations conferring resistance to ACCase-inhibiting herbicides in Lolium spp.

Reverte, R. A., Hanley, S. J., Kaundun, S. S., Karp, A. and Moss, S. R. 2013. A SNaPshot assay for the rapid and simple detection of known point mutations conferring resistance to ACCase-inhibiting herbicides in Lolium spp. Weed Research. 53 (1), pp. 12-20. https://doi.org/10.1111/wre.12002

The presence of two different target-site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molcular test for the characterisation of six ALS and seven ACCase SNPs

Marshall, R., Hanley, S. J., Hull, R. I. and Moss, S. R. 2013. The presence of two different target-site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molcular test for the characterisation of six ALS and seven ACCase SNPs. Pest Management Science. 69 (6), pp. 727-737. https://doi.org/10.1002/ps.3429

High levels of genetic diversity in Salix viminalis of the Czech Republic as revealed by microsatellite markers

Trybush, S. O., Jahodova, S., Cizkova, L., Karp, A. and Hanley, S. J. 2012. High levels of genetic diversity in Salix viminalis of the Czech Republic as revealed by microsatellite markers. BioEnergy Research. 5 (4), pp. 969-977. https://doi.org/10.1007/s12155-012-9212-4

Population genetics of Tuberolachnus salignus, an obligate parthenogenetic aphid

Aradottir, G. I., Hanley, S. J., Collins, C. M., Dawson, K. J., Karp, A., Leather, S. R., Shield, I. F. and Harrington, R. 2012. Population genetics of Tuberolachnus salignus, an obligate parthenogenetic aphid. Agricultural and Forest Entomology. 14 (2), pp. 197-205. https://doi.org/10.1111/j.1461-9563.2011.00559.x

Genetic mapping of rust resistance loci in biomass willow

Hanley, S. J., Pei, M. H., Powers, S. J., Ruiz, C., Mallott, M. D., Barker, J. H. A. and Karp, A. 2011. Genetic mapping of rust resistance loci in biomass willow. Tree Genetics & Genomes. 7 (3), pp. 597-608. https://doi.org/10.1007/s11295-010-0359-x

Condensed tannins in willow (Salix spp.): a first step to evaluate novel feeds for nutritionally improved animal products

Falchero, L., Brown, R. H., Mueller-Harvey, I., Hanley, S. J., Shield, I. F. and Karp, A. 2011. Condensed tannins in willow (Salix spp.): a first step to evaluate novel feeds for nutritionally improved animal products. Hopkins, A., Krautzer, B. and Potsch, E. M. (ed.) Proceedings 16th Symposium of the European Grassland Federation: Grassland Farming and Land Management Systems in Mountainous Regions, Gumpenstein, 29-31 August 2011 . Wallig Ennstaler Druckerei und Verlag, Grobming. pp. 434-436

The structural diversity of condensed tannins in willows (salix spp.): a first screening to improve the nutritional quality of ruminant products

Falchero, L., Brown, R., Hanley, S. J., Karp, A., Shield, I. F. and Mueller-Harvey, I. 2011. The structural diversity of condensed tannins in willows (salix spp.): a first screening to improve the nutritional quality of ruminant products.

BSBEC-BioMASS - selecting traits to optimise biomass yield of SRC willow

Cunniff, J., Shield, I. F., Barraclough, T. J. P., Castle, M., Hanley, S. J., Andralojc, P. J., Richter, G. M., Cerasuolo, M., Purdy, S., Clifton-Brown, J., Maddison, A., Jones, L., Donnison, I. and Karp, A. 2011. BSBEC-BioMASS - selecting traits to optimise biomass yield of SRC willow. Aspects of Applied Biology. 112.

Willow

Hanley, S. J. 2011. Willow. in: Karp, A. and Halford, N. G. (ed.) Energy crops Royal Society of Chemistry (RSC), Cambridge. pp. 259-274

Genetic improvement of willow for bioenergy and biofuels

Karp, A., Hanley, S. J., Trybush, S. O., Macalpine, W. J., Pei, M. H. and Shield, I. F. 2011. Genetic improvement of willow for bioenergy and biofuels. Journal of Integrative Plant Biology. 53 (2), pp. 151-165. https://doi.org/10.1111/j.1744-7909.2010.01015.x

QTL mapping of enzymatic saccharification in short rotation coppice willow and its independence from biomass yield

Brereton, N. J. B., Pitre, F. E., Hanley, S. J., Ray, M. J., Karp, A. and Murphy, R. J. 2010. QTL mapping of enzymatic saccharification in short rotation coppice willow and its independence from biomass yield. BioEnergy Research. 3 (3), pp. 251-261. https://doi.org/10.1007/s12155-010-9077-3

Host selection of the giant willow aphid (Tuberolachnus salignus)

Aradottir, G. I., Karp, A., Hanley, S. J., Shield, I. F., Woodcock, C. M., Dewhirst, S. Y., Collins, M., Leather, S. and Harrington, R. 2009. Host selection of the giant willow aphid (Tuberolachnus salignus).  REDIA, XCII, 2009: 223-225.

An anchored linkage map for sugar beet based on AFLP, SNP and RAPD markers and QTL mapping of a new source of resistance to Beet necrotic yellow vein virus

Grimmer, M. K., Trybush, S., Hanley, S. J., Francis, S. A., Karp, A. and Asher, M. J. C. 2007. An anchored linkage map for sugar beet based on AFLP, SNP and RAPD markers and QTL mapping of a new source of resistance to Beet necrotic yellow vein virus. Theoretical and Applied Genetics. 114 (7), pp. 1151-1160. https://doi.org/10.1007/s00122-007-0507-3

Getting the most out of fluorescent amplified fragment length polymorphism

Trybush, S., Hanley, S. J., Cho, K-H., Jahodova, S., Grimmer, M., Emelianov, I., Bayon, C. and Karp, A. 2006. Getting the most out of fluorescent amplified fragment length polymorphism. Canadian Journal Of Botany. 84 (8), pp. 1347-1354. https://doi.org/10.1139/B06-096

Alignment of a Salix linkage map to the Populus genomic sequence reveals macrosynteny between willow and poplar genomes

Hanley, S. J., Mallott, M. D. and Karp, A. 2006. Alignment of a Salix linkage map to the Populus genomic sequence reveals macrosynteny between willow and poplar genomes. Tree Genetics & Genomes. 3 (1), pp. 35-48. https://doi.org/10.1007/s11295-006-0049-x

Genetic mapping of important agronomic traits in biomass willow

Hanley, S. J. 2003. Genetic mapping of important agronomic traits in biomass willow. F - Theses

A genetic linkage map of willow (Salix viminalis) based on AFLP and microsatellite markers

Hanley, S. J., Barker, J. H. A., Van Ooijen, J. W., Aldam, C., Harris, S. L., Ahman, I., Larsson, S. and Karp, A. 2002. A genetic linkage map of willow (Salix viminalis) based on AFLP and microsatellite markers. Theoretical and Applied Genetics. 105 (6-7), pp. 1087-1096. https://doi.org/10.1007/s00122-002-0979-0

Isolation and characterization of microsatellites in Echinochloa (L.) Beauv. spp.

Danquah, E. Y., Hanley, S. J., Brookes, R. C., Aldam, C. and Karp, A. 2002. Isolation and characterization of microsatellites in Echinochloa (L.) Beauv. spp. Molecular Ecology Notes. 2 (1), pp. 54-56. https://doi.org/10.1046/j.1471-8286.2002.00144.x

Identification of transposon-tagged genes by the random sequencing of Mutator -tagged DNA fragments from Zea mays

Hanley, S. J., Edwards, D., Stevenson, D., Haines, S., Hegarty, M., Schuch, W. and Edwards, K. J. 2000. Identification of transposon-tagged genes by the random sequencing of Mutator -tagged DNA fragments from Zea mays. The Plant Journal. 23, pp. 557-566.