The transfer of genetic information in crop plants by somatic fusion of protoplasts.

G - Articles in popular magazines and other technical publications

Jones, M. G. K. and Bright, S. W. J. 1986. The transfer of genetic information in crop plants by somatic fusion of protoplasts.

Authors	Jones, M. G. K. and Bright, S. W. J.
Year of Publication	1986
Open access	Published as non-open access
Collective title	Biomolecular engineering in the European Community (1982-86) - Final Report. Ed. E. Magnien. Dordrecht : Nijhoff
Page range	906-913

Related outputs

Top 10 plant-parasitic nematodes in molecular plant pathology

A - Papers appearing in refereed journals

Jones, J. T., Haegeman, A., Danchin, E. G. J., Gaur, H. S., Helder, J., Jones, M. G. K., Kikuchi, T., Manzanilla-Lopez, R. H., Palomares-Rius, J. E., Wesemael, W. M. L. and Perry, R. N. 2013. Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology. 14 (9), pp. 946-961. https://doi.org/10.1111/mpp.12057

A method based on PCR for the construction of cDNA libraries and probes from small amounts of tissue

A - Papers appearing in refereed journals

Bertioli, D. J., Smoker, M., Brown, A. C. P., Jones, M. G. K. and Burrows, P. R. 1994. A method based on PCR for the construction of cDNA libraries and probes from small amounts of tissue. BioTechniques. 16, pp. 1054-1058.

Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and s. tuberosum using non-radioactive digoxigenin-labeled DNA probes

A - Papers appearing in refereed journals

Xu, Y-S., Jones, M. G. K., Karp, A. and Pehu, E. 1993. Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and s. tuberosum using non-radioactive digoxigenin-labeled DNA probes. Theoretical and Applied Genetics. 85 (8), pp. 1017-1022. https://doi.org/10.1007/BF00215042

Cellular and molecular approaches to the control of plant parasitic nematodes

B - Book chapters etc edited externally

Burrows, P. R. and Jones, M. G. K. 1993. Cellular and molecular approaches to the control of plant parasitic nematodes. in: Evans, K., Webster, J. M. and Trudgill, D. L. (ed.) Plant parasitic nematodes in temperate agriculture CABI International, Wallingford, Oxon (CABI). pp. 609-630

Transient expression of foreign genes introduced into barley endosperm protoplasts by PEG-mediated transfer or into intact endosperm tissue by microprojectile bombardment

A - Papers appearing in refereed journals

Lee, B. T., Murdoch, K., Topping, J., Jones, M. G. K. and Kreis, M. 1991. Transient expression of foreign genes introduced into barley endosperm protoplasts by PEG-mediated transfer or into intact endosperm tissue by microprojectile bombardment. Plant Science. 78, pp. 237-246.

Resistance in Solanum brevidens to both potato virus Y and potato virus X may be associated with slow cell-to-cell spread

A - Papers appearing in refereed journals

Valkonen, J. P. T., Pehu, E., Jones, M. G. K. and Gibson, R. W. 1991. Resistance in Solanum brevidens to both potato virus Y and potato virus X may be associated with slow cell-to-cell spread. Journal of General Virology. 72 (2), pp. 231-236. https://doi.org/10.1099/0022-1317-72-2-231

Plant regeneration from protoplasts of Solanum species with potential agricultural value (S. hjertingii, S. polyadenium, S. capsicibaccatum)

A - Papers appearing in refereed journals

Xu, Y-S., Pehu, E., Malone, R. and Jones, M. G. K. 1991. Plant regeneration from protoplasts of Solanum species with potential agricultural value (S. hjertingii, S. polyadenium, S. capsicibaccatum). Plant Cell Reports. 9, pp. 520-522.

An assessment of genetic stability of potato in vitro by molecular and phenotypic analysis

A - Papers appearing in refereed journals

Potter, R. and Jones, M. G. K. 1991. An assessment of genetic stability of potato in vitro by molecular and phenotypic analysis. Plant Science. 76, pp. 239-248.

Species-specific sequences in the genus solanum - identification, characterization, and application to study somatic hybrids of solanum-brevidens and solanum-tuberosum

A - Papers appearing in refereed journals

Pehu, E., Thomas, M., Poutala, T., Karp, A. and Jones, M. G. K. 1990. Species-specific sequences in the genus solanum - identification, characterization, and application to study somatic hybrids of solanum-brevidens and solanum-tuberosum. Theoretical and Applied Genetics. 80 (5), pp. 693-698. https://doi.org/10.1007/BF00224231

Application of species specific repetitive DNA sequences to study nuclear hybridity of somatic fusion products at the callus level

C2 - Non-edited contributions to conferences

Poutala, R. T., Jones, M. G. K., Malone, R., Thomas, M. and Pehu, E. 1990. Application of species specific repetitive DNA sequences to study nuclear hybridity of somatic fusion products at the callus level. European Association of Potato Research Meeting, Edinburgh, 1990. Abstract. pp. 14-15

The application of protoplast technology to potato improvement

C2 - Non-edited contributions to conferences

Jones, M. G. K., Jones, H., Dunckley, R., Karp, A. and Pehu, E. 1990. The application of protoplast technology to potato improvement. European Association of Potato Research Meeting, Edinburgh, 1990. Abstract. pp. 96

Stable transformation of Solanum protoplasts by direct gene transfer

C2 - Non-edited contributions to conferences

Xu, Y-S., Jones, H. and Jones, M. G. K. 1990. Stable transformation of Solanum protoplasts by direct gene transfer. European Association of Potato Research Meeting, Edinburgh, 1990. Abstract. pp. 439

Plant regeneration from protoplasts of wild Solanum species

C2 - Non-edited contributions to conferences

Xu, Y-S., Pehu, E., Dunckley, R. and Jones, M. G. K. 1990. Plant regeneration from protoplasts of wild Solanum species. European Association of Potato Research Meeting, Edinburgh, 1990. Abstract. pp. 440

Resistance in Solanum brevidens to potato virus Y and potato virus X is associated with slow cell-to-cell spread.

C2 - Non-edited contributions to conferences

Valkonen, J. P. T., Pehu, E., Jones, M. G. K. and Gibson, R. W. 1990. Resistance in Solanum brevidens to potato virus Y and potato virus X is associated with slow cell-to-cell spread. European Association of Potato Research Meeting, Edinburgh, 1990. Abstract. pp. 474-475

Application of linkage group specific RFLPs to study assymetric hybrids of S. tuberosum and S. brevidens

C2 - Non-edited contributions to conferences

Pehu, E., Malone, R., Karp, A. and Jones, M. G. K. 1990. Application of linkage group specific RFLPs to study assymetric hybrids of S. tuberosum and S. brevidens. European Association of Potato Research Meeting, Edinburgh, 1990. Abstract. pp. 13

Resistance to potato virus Y and potato virus X in Solanum brevidens.

A - Papers appearing in refereed journals

Gibson, R. W., Pehu, E., Woods, R. D. and Jones, M. G. K. 1990. Resistance to potato virus Y and potato virus X in Solanum brevidens. Annals of Applied Biology - AAB. 116 (1), pp. 151-156. https://doi.org/10.1111/j.1744-7348.1990.tb06594.x

Studies on the genetic basis of resistance to potato leaf roll virus, potato virus Y and potato virus X in Solanum brevidens using somatic hybrids of Solanum brevidens and Solanum tuberosum.

A - Papers appearing in refereed journals

Pehu, E., Gibson, R. W., Jones, M. G. K. and Karp, A. 1990. Studies on the genetic basis of resistance to potato leaf roll virus, potato virus Y and potato virus X in Solanum brevidens using somatic hybrids of Solanum brevidens and Solanum tuberosum. Plant Science. 69 (1), pp. 95-101. https://doi.org/10.1016/0168-9452(90)90107-y

Electroporation of cells.

A - Papers appearing in refereed journals

Lindsey, K. and Jones, M. G. K. 1990. Electroporation of cells. Physiologia Plantarum. 79 (1), pp. 168-172.

Selection of transformed cells.

B - Book chapters etc edited externally

Lindsey, K. and Jones, M. G. K. 1990. Selection of transformed cells. in: Plant cell line selection: procedures and applications. P.J. Dix, ed. VCH, Weinheim.. pp. 317-339

Genetic engineering and its implications for crop production.

C2 - Non-edited contributions to conferences

Jones, M. G. K. 1989. Genetic engineering and its implications for crop production. Proceedings of the Institute of Biology Symposium on New Biological Developments in the Production and Processing of Food, Greensborough College, N. I., 1989. p.1-2..

Molecular, cytogenetic and morphological characterization of somatic hybrids of dihaploid Solanum tuberosum and diploid S. brevidens.

A - Papers appearing in refereed journals

Pehu, E., Karp, A., Moore, K., Steele, S., Dunckley, R. and Jones, M. G. K. 1989. Molecular, cytogenetic and morphological characterization of somatic hybrids of dihaploid Solanum tuberosum and diploid S. brevidens. Theoretical and Applied Genetics. 78 (5), pp. 696-704. https://doi.org/10.1007/BF00262566

Cytogenetical analysis of parental genome stabilities in somatic hybrids of Solanum tuberosum and S. brevidens using species-specific probes.

C2 - Non-edited contributions to conferences

Pehu, E., Karp, A., Thomas, M., Clarke, M. and Jones, M. G. K. 1989. Cytogenetical analysis of parental genome stabilities in somatic hybrids of Solanum tuberosum and S. brevidens using species-specific probes. In Proceedings of the 10th International Chromosome Symposium, Uppsala, 1989, Abstract p 103.

Plant biotechnology in agriculture. 300pp.

E - Books edited/written by staff member, alone/with others

Lindsey, K. and Jones, M. G. K. 1989. Plant biotechnology in agriculture. 300pp.

Improving potatoes.

C2 - Non-edited contributions to conferences

Jones, M. G. K., Dunckley, R., Steele, S., Pehu, E., Karp, A. and Gibson, R. W. 1989. Improving potatoes.  Proceedings of the International Biological Conference, Leicester, 1989. Abstract p.7..

Isolation and culture of protoplasts from specific tissues: dedifferentiation and regeneration.

C2 - Non-edited contributions to conferences

Jones, M. G. K. 1989. Isolation and culture of protoplasts from specific tissues: dedifferentiation and regeneration.  Proceedings of the EEC Workshop on Fundamental Mechanisms of Plant Regeneration, Dourdan, 1989. Abstract p.7..

Molecular, pathological and field evaluation of somatic hybrids between potato and Solanum brevidens .

C2 - Non-edited contributions to conferences

Jones, M. G. K. 1989. Molecular, pathological and field evaluation of somatic hybrids between potato and Solanum brevidens . Proceedings of the Meeting on Control of Morphogenesis, Aix-le-Bains, 1989. F. White ed. Abstract p.62. .

Transfer of resistance to potato viruses by somatic hybridisation and field assessment under AGGM guidelines.

C2 - Non-edited contributions to conferences

Jones, M. G. K., Dunckley, R., Pehu, E. and Gibson, R. W. 1989. Transfer of resistance to potato viruses by somatic hybridisation and field assessment under AGGM guidelines. Proceedings of the University of Nottingham 49th Easter School on Genetic Engineering of Crop Plants p.18..

Shotgun cloning by direct gene transfer: optimisation of DNA transfer conditions.

C2 - Non-edited contributions to conferences

Gallois, P., Lindsey, K., Jones, M. G. K. and Kreis, M. 1989. Shotgun cloning by direct gene transfer: optimisation of DNA transfer conditions.

Identification and characterisation of Solanum brevidens and Solanum tuberosum specific DNA sequences and their use in a somatic hybridisation programme.

C2 - Non-edited contributions to conferences

Pehu, E., Karp, A., Gibson, R. W., Thomas, M., Malone, R. and Jones, M. G. K. 1989. Identification and characterisation of Solanum brevidens and Solanum tuberosum specific DNA sequences and their use in a somatic hybridisation programme. Proceedings of the 1st International Symposium on the Molecular Biology of thge Potato, Maine. Abstract p.67..

Cytogenetical analysis of parental genome stabilities in somatic hybrids of Solanum tuberosum and S. brevidens using species-specific probes.

C2 - Non-edited contributions to conferences

Pehu, E., Gibson, R. W., Karp, A. and Jones, M. G. K. 1989. Cytogenetical analysis of parental genome stabilities in somatic hybrids of Solanum tuberosum and S. brevidens using species-specific probes. Proceedings of the 1st International Symposium on the Molecular Biology of thge Potato, Maine. Abstract p.38..

Tissue specific expression during cereal grain development.

A - Papers appearing in refereed journals

Topping, J., Marris, C., Gallois, P., Lee, B. T., Murdoch, K., Jones, M. G. K. and Kreis, M. 1989. Tissue specific expression during cereal grain development. Journal Of Cellular Biochemistry. 12C, p. 205.

Stable transformation of sugar beet protoplasts by electroporation.

A - Papers appearing in refereed journals

Lindsey, K. and Jones, M. G. K. 1989. Stable transformation of sugar beet protoplasts by electroporation. Plant Cell Reports. 8, pp. 71-74.

Introduction and expression of foreign genes in wheat protoplasts.

G - Articles in popular magazines and other technical publications

Lee, B. T., Topping, J., Kreis, M. and Jones, M. G. K. 1989. Introduction and expression of foreign genes in wheat protoplasts. Annual Wheat Newsletter. 351, p. 123.

Variability in potato tissue culture

A - Papers appearing in refereed journals

Karp, A., Jones, M. G. K., Foulger, D., Fish, N. and Bright, S. W. J. 1989. Variability in potato tissue culture. American potato journal. 66 (10), pp. 669-684. https://doi.org/10.1007/bf02853986

Genetic manipulation of arable crop plants by protoplast fusion and direct gene transfer.

A - Papers appearing in refereed journals

Jones, M. G. K., Jones, H., Lee, B. T., Lindsey, K., Potter, R. H., Karp, A. and Pehu, E. 1989. Genetic manipulation of arable crop plants by protoplast fusion and direct gene transfer. Journal Of Cellular Biochemistry. 13D (Supplement), p. 262.

Transient gene expression in electroporated Solanum protoplasts.

A - Papers appearing in refereed journals

Jones, H., Ooms, G. and Jones, M. G. K. 1989. Transient gene expression in electroporated Solanum protoplasts. Plant Molecular Biology. 13, pp. 503-511.

Direct gene transfer into plant protoplasts.

A - Papers appearing in refereed journals

Jones, H. and Jones, M. G. K. 1989. Direct gene transfer into plant protoplasts. Plants Today. 2, pp. 175-178.

Isolation, culture and regeneration of plants from potato protoplasts

A - Papers appearing in refereed journals

Jones, H., Karp, A. and Jones, M. G. K. 1989. Isolation, culture and regeneration of plants from potato protoplasts. Plant Cell Reports. 8 (5), pp. 307-311. https://doi.org/10.1007/BF00274137

Genetic manipulation of arable crop plants - present status and future prospects. Sir Thomas Middleton Memorial Lecture.

B - Book chapters etc edited externally

Jones, M. G. K. 1989. Genetic manipulation of arable crop plants - present status and future prospects. Sir Thomas Middleton Memorial Lecture. in:

Genetic manipulation of arable crop plants - present status and future prospects.

A - Papers appearing in refereed journals

Jones, M. G. K. 1989. Genetic manipulation of arable crop plants - present status and future prospects. Agricultural Progress. 64, pp. 1-18.

A method for large-scale progeny screening of putative transformed cereal.

A - Papers appearing in refereed journals

Lee, B., Murdoch, K., Kreis, M. and Jones, M. G. K. 1989. A method for large-scale progeny screening of putative transformed cereal. Plant Molecular Biology Reporter. 7, pp. 129-134.

Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat.

A - Papers appearing in refereed journals

Lee, B., Murdoch, K., Topping, J., Kreis, M. and Jones, M. G. K. 1989. Transient gene expression in aleurone protoplasts isolated from developing caryopses of barley and wheat. Plant Molecular Biology. 13, pp. 21-29.

The 15th Congress of the Scandinavian Society for Plant Physiology (Abstracts). Variation in chromosome-number in somatic hybrids of dihaploid potato solanum tuberosum and solanum brevidens

A - Papers appearing in refereed journals

Pehu, E., Potter, R., Karp, A. and Jones, M. G. K. 1988. The 15th Congress of the Scandinavian Society for Plant Physiology (Abstracts). Variation in chromosome-number in somatic hybrids of dihaploid potato solanum tuberosum and solanum brevidens. Physiologia Plantarum. 73 (2), pp. A22-A22. https://doi.org/10.1111/j.1399-3054.1988.tb00585.x

Direct gene transfer into plant protoplasts.

C1 - Edited contributions to conferences/learned societies

Lindsey, K., Jones, M. G. K. and Fish, N. 1988. Direct gene transfer into plant protoplasts. Methods in Molecular Biology .

Engineering somatic hybrid potatoes.

G - Articles in popular magazines and other technical publications

Fish, N. and Jones, M. G. K. 1988. Engineering somatic hybrid potatoes. Plants Today. 1 (2), pp. 47-49.

Transfer of disease resistance in potato through asymmetric hybridization.

C2 - Non-edited contributions to conferences

Malone, R. P., Cooper-Bland, S. and Jones, M. G. K. 1988. Transfer of disease resistance in potato through asymmetric hybridization. Abstracts Eucarpia Meeting on Genetic Manipulation in Plant Breeding, Elsinore, Denmark, 1988, 108.

Restriction fragment analysis to assess stability of potato in vitro .

C2 - Non-edited contributions to conferences

Potter, R. and Jones, M. G. K. 1988. Restriction fragment analysis to assess stability of potato in vitro . Abstracts Eucarpia Meeting on Genetic Manipulation in Plant Breeding, Elsinore, Denmark, 1988, 48.

Chromosome number and parental nuclear and chloroplast DNA contributions in somatic hybrids of dihaploid S. tuberosum and S. brevidens .

C2 - Non-edited contributions to conferences

Pehu, E., Karp, A. and Jones, M. G. K. 1988. Chromosome number and parental nuclear and chloroplast DNA contributions in somatic hybrids of dihaploid S. tuberosum and S. brevidens . Abstracts Eucarpia Meeting on Genetic Manipulation in Plant Breeding, Elsinore, Denmark, 1988, 111. .

Variation in chromosome number in somatic hybrids of diploid potato (Solanum tuberosum and S. brevidens ).

C2 - Non-edited contributions to conferences

Pehu, E., Potter, R., Karp, A. and Jones, M. G. K. 1988. Variation in chromosome number in somatic hybrids of diploid potato (Solanum tuberosum and S. brevidens ).  Proceedings 15th Congress of the Scandanavian Society of Plant Physiology, Turku, Finland, 1988, 22A.. Wiley.

Morphogenesis in protoplast-derived callus of wheat.

G - Articles in popular magazines and other technical publications

Jones, M. G. K. and Wu, Q. S. 1988. Morphogenesis in protoplast-derived callus of wheat. Annual Wheat Newsletter. 34, p. 97.

Chromosome variation in dividing protoplasts and cell suspensions of wheat.

G - Articles in popular magazines and other technical publications

Karp, A., Wu, Q. S., Steele, S. H. and Jones, M. G. K. 1988. Chromosome variation in dividing protoplasts and cell suspensions of wheat. Annual Wheat Newsletter. 34, p. 97.

Cis and trans-acting elements involved in cell-type specific gene expression within the barley endosperm.

C2 - Non-edited contributions to conferences

Marris, C., Bianchi, M., Clark, J., Forde, J., Lee, B., Jones, H., Topping, J., Murdoch, K., Jones, M. G. K. and Kreis, M. 1988. Cis and trans-acting elements involved in cell-type specific gene expression within the barley endosperm. Abstracts 2nd International congress plant molecular biology, Jerusalem, 1988, 303.

Molecular characterisation of somatic hybrids of Solanum tuberosum and S. brevidens.

C2 - Non-edited contributions to conferences

Pehu, E., Clark, M., Karp, A. and Jones, M. G. K. 1988. Molecular characterisation of somatic hybrids of Solanum tuberosum and S. brevidens. Abstracts 2nd International congress plant molecular biology, Jerusalem, 1988, 432.

A comparison of tissue culture response between related tetraploid and dihaploid S. tuberosum genotypes.

A - Papers appearing in refereed journals

Fish, N. and Jones, M. G. K. 1988. A comparison of tissue culture response between related tetraploid and dihaploid S. tuberosum genotypes. Plant Cell, Tissue and Organ Culture. 15, pp. 201-210.

Field assessment of dihaploid Solanum tuberosum and S. brevidens somatic hybrids.

A - Papers appearing in refereed journals

Fish, N., Steele, S. H. and Jones, M. G. K. 1988. Field assessment of dihaploid Solanum tuberosum and S. brevidens somatic hybrids. Theoretical and Applied Genetics. 76, pp. 880-886,.

Plant tissue culture.

C1 - Edited contributions to conferences/learned societies

Jones, M. G. K., Fish, N. and Lindsey, K. 1988. Plant tissue culture. Methods in Molecular Biology .

Plant protoplast fusion.

C1 - Edited contributions to conferences/learned societies

Fish, N., Lindsey, K. and Jones, M. G. K. 1988. Plant protoplast fusion. Methods in Molecular Biology .

Introduction and expression of foreign genes in cereals.

C1 - Edited contributions to conferences/learned societies

Jones, M. G. K. 1988. Introduction and expression of foreign genes in cereals. Cereal quality. Aspects of Applied Biology 15 , 569-580..

Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solanum tuberosum and S. brevidens

A - Papers appearing in refereed journals

Gibson, R. W., Jones, M. G. K. and Fish, N. 1988. Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid Solanum tuberosum and S. brevidens. Theoretical and Applied Genetics. 76 (1), pp. 113-117. https://doi.org/10.1007/BF00288840

Plant biotechnology.

B - Book chapters etc edited externally

Jones, M. G. K. and Lindsey, K. 1988. Plant biotechnology. in: Molecular biology and biotechnology. 2nd ed. Eds J.M. Walker & E.B. Gingold. London : Royal Society of Chemistry pp. 117-130

Isolation, culture and morphogenesis from wheat protoplasts, and study of expression of DNA constructs by direct gene transfer

A - Papers appearing in refereed journals

Lee, B. T., Murdoch, K., Topping, J., De Both, M. T. J., Wu, Q. S., Karp, A., Steele, S., Symonds, C., Kreis, M. and Jones, M. G. K. 1988. Isolation, culture and morphogenesis from wheat protoplasts, and study of expression of DNA constructs by direct gene transfer. Plant Cell, Tissue and Organ Culture. 12 (2), pp. 223-226. https://doi.org/10.1007/bf00040091

Production of somatic hybrids by electrofusion in Solanum

A - Papers appearing in refereed journals

Fish, N., Karp, A. and Jones, M. G. K. 1988. Production of somatic hybrids by electrofusion in Solanum. Theoretical and Applied Genetics. 76 (2), pp. 260-266. https://doi.org/10.1007/BF00257854

Test-tube improvements of arable crops developments for the 21st century.

G - Articles in popular magazines and other technical publications

Karp, A., Jones, M. G. K. and Ooms, G. 1988. Test-tube improvements of arable crops developments for the 21st century.

Tissue specific gene expression during cereal grain development.

A - Papers appearing in refereed journals

Topping, J., Marris, C., Gallois, P., Lee, B. T., Murdoch, K. M., Jones, M. G. K. and Kreis, M. 1988. Tissue specific gene expression during cereal grain development. Journal Of Cellular Biochemistry. 12C (Supplement), p. 205.

Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture.

A - Papers appearing in refereed journals

Morris, P. C., Bowles, D. J., Maddock, S. E., Jones, M. G. K., Lenten, J. R. and Weiler, E. W. 1988. Determination of endogenous abscisic acid levels in immature cereal embryos during in vitro culture. Planta. 173, pp. 110-116.

Fusing plant protoplasts.

A - Papers appearing in refereed journals

Jones, M. G. K. 1988. Fusing plant protoplasts. Trends in Biotechnology. 6, pp. 153-158.

Improvement of the seed protein-quality of barley + wheat by genetic-engineering

A - Papers appearing in refereed journals

Shewry, P. R., Tatham, A. S., Karp, A. and Jones, M. G. K. 1987. Improvement of the seed protein-quality of barley + wheat by genetic-engineering. Cereal Foods World. 32 (9), pp. 674-674.

Recent advances in plant electroporation.

B - Book chapters etc edited externally

Jones, H., Tempelaar, M. J. and Jones, M. G. K. 1987. Recent advances in plant electroporation. in:

Modulation and direction of the electrofusion response in plant protoplasts.

A - Papers appearing in refereed journals

Tempelaar, M. J., Duyst, A., De Vlas, S. Y., Krol, G., Symmonds, C. and Jones, M. G. K. 1987. Modulation and direction of the electrofusion response in plant protoplasts. Plant Science. 48, pp. 99-105.

Chromosome variation in dividing protoplasts and cell suspensions of wheat.

A - Papers appearing in refereed journals

Karp, A., Wu, Q. S., Steele, S. H. and Jones, M. G. K. 1987. Chromosome variation in dividing protoplasts and cell suspensions of wheat. Theoretical and Applied Genetics. 74 (1), pp. 140-146. https://doi.org/10.1007/bf00290096

Barley mutants lacking glutamine synthetase - biochemical and genetic analysis.

A - Papers appearing in refereed journals

Wallsgrove, R. M., Turner, J. C., Hall, N. P., Kendall, A. C. and Bright, S. W. J. 1987. Barley mutants lacking glutamine synthetase - biochemical and genetic analysis. Plant Physiology. 83, pp. 155-158.

Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion.

A - Papers appearing in refereed journals

De Vries, S. E., Jacobsen, E., Jones, M. G. K., Loonen, A. E. H.M., Tempelaar, M. J., Wijbrandi, J. and Feenstra, W. J. 1987. Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion. Theoretical and Applied Genetics. 73, pp. 451-458.

Relative stability among barley plants regenerated from cultured immature embryos

A - Papers appearing in refereed journals

Karp, A., Steele, S. H., Parmar, S., Jones, M. G. K., Shewry, P. R. and Breiman, A. 1987. Relative stability among barley plants regenerated from cultured immature embryos. Genome. 29 (3), pp. 405-412. https://doi.org/10.1139/g87-071

Biochemical and molecular genetics of nitrogen assimilation and re-assimilation in barley.

G - Articles in popular magazines and other technical publications

Wallsgrove, R. M., Kendall, A. C., Hall, N. P., Turner, J. and Bright, S. W. J. 1987. Biochemical and molecular genetics of nitrogen assimilation and re-assimilation in barley. Journal of the Science of Food and Agriculture. 28 (4), pp. 318-319.

Dividing protoplasts of wheat and their use for introducing foreign genes.

G - Articles in popular magazines and other technical publications

Wu, Q. S., Deboth, M. T. J. and Jones, M. G. K. 1987. Dividing protoplasts of wheat and their use for introducing foreign genes. Annual Wheat Newsletter. 33, p. 102.

Role of ABA in the regulation of gene expression during cereal embryogenesis.

G - Articles in popular magazines and other technical publications

Morris, P. C., Weiler, E. M., Jones, M. G. K. and Bowles, D. J. 1987. Role of ABA in the regulation of gene expression during cereal embryogenesis.

Studies on gene transfer into cereals.

G - Articles in popular magazines and other technical publications

Lee, B. T., Deboth, M. T. J., Murdoch, K., Topping, J., Wu, Q. S., Steele, S. H., Kreis, M. and Jones, M. G. K. 1987. Studies on gene transfer into cereals.

Cytology of wheat suspensions and cultured protoplasts.

G - Articles in popular magazines and other technical publications

Karp, A., Wu, Q. S. and Jones, M. G. K. 1987. Cytology of wheat suspensions and cultured protoplasts. Annual Wheat Newsletter. 33, p. 102.

Potato protoplasts and tissue culture in crop improvement

A - Papers appearing in refereed journals

Karp, A., Jones, M. G. K., Foulger, D., Ooms, G. and Bright, S. W. J. 1987. Potato protoplasts and tissue culture in crop improvement. Biotechnology and genetic engineering reviews. 5 (1), pp. 1-32. https://doi.org/10.1080/02648725.1987.10647833

Production and characterisation of somatic hybrids of potato (Solanum sp.).

G - Articles in popular magazines and other technical publications

Jones, M. G. K. and Fish, N. 1987. Production and characterisation of somatic hybrids of potato (Solanum sp.).

The application of cell biological techniques to crop improvement.

G - Articles in popular magazines and other technical publications

Jones, M. G. K. 1987. The application of cell biological techniques to crop improvement.

Use of protoplast fusion and somaclonal, variation in potato breeding.

G - Articles in popular magazines and other technical publications

Jones, M. G. K. 1987. Use of protoplast fusion and somaclonal, variation in potato breeding.

Electrofusion and electroporation of crop plant protoplasts.

G - Articles in popular magazines and other technical publications

Jones, M. G. K., Karp, A., Jones, H., Cooper-Bland, S., Lindsey, K., Wu, Q. S., Lee, B. T., Deboth, M. T. J. and Fish, N. 1987. Electrofusion and electroporation of crop plant protoplasts.

Barley photorespiration mutants.

G - Articles in popular magazines and other technical publications

Kendall, A. C., Bright, S. W. J., Hall, N. P., Keys, A. J., Lea, P. J., Turner, J. C. and Wallsgrove, R. M. 1987. Barley photorespiration mutants.

Isolation, culture and morphogenesis from wheat protoplasts and study of expression of DNA constructs by direct gene transfer.

G - Articles in popular magazines and other technical publications

Lee, B. T., Murdoch, K., Topping, J., Deboth, M. T. J., Wu, Q. S., Karp, A., Steele, S., Symonds,C., Kreis, M. and Jones, M. G. K. 1987. Isolation, culture and morphogenesis from wheat protoplasts and study of expression of DNA constructs by direct gene transfer.

Transient expression of genes electroporated into protoplasts of cultivated and wild potato.

G - Articles in popular magazines and other technical publications

Jones, H., Symonds, C. J. and Jones, M. G. K. 1987. Transient expression of genes electroporated into protoplasts of cultivated and wild potato.

Electrofusion and electroporation of sugar beet protoplasts.

G - Articles in popular magazines and other technical publications

Eady, C., Warren, G., Lindsey, K. and Jones, M. G. K. 1987. Electrofusion and electroporation of sugar beet protoplasts.

Electroporation of tobacco protoplasts as a model system to study plant gene expression.

G - Articles in popular magazines and other technical publications

Deboth, M. T. J. and Jones, M. G. K. 1987. Electroporation of tobacco protoplasts as a model system to study plant gene expression.

Introduction and expression of foreign genes in cereals.

G - Articles in popular magazines and other technical publications

Jones, M. G. K. 1987. Introduction and expression of foreign genes in cereals.

The permeability of electroporated cells and protoplasts of sugar beet.

A - Papers appearing in refereed journals

Lindsey, K. and Jones, M. G. K. 1987. The permeability of electroporated cells and protoplasts of sugar beet. Planta. 172, pp. 346-355.

Electrofusion of biochemically well-characterised nitrate reductase deficient Nicotiana plumbaginifolia mutants. Studies on optimization and complementation.

A - Papers appearing in refereed journals

De Vries, S. E., Jacobsen, E., Jones, M. G. K., Loonen, A. E. H.M., Tempelaar, M. J., Wijbrandi, J. and Feenstra, W. J. 1987. Electrofusion of biochemically well-characterised nitrate reductase deficient Nicotiana plumbaginifolia mutants. Studies on optimization and complementation. Plant Science. 51, pp. 105-112.

Transient gene expression in electroporated protoplasts and intact cells of sugar beet.

A - Papers appearing in refereed journals

Lindsey, K. and Jones, M. G. K. 1987. Transient gene expression in electroporated protoplasts and intact cells of sugar beet. Plant Molecular Biology. 10, pp. 43-52.

Improved isolation of dihaploid solanum tubersoum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens

A - Papers appearing in refereed journals

Fish, N., Karp, A. and Jones, M. G. K. 1987. Improved isolation of dihaploid solanum tubersoum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens. In Vitro Cellular and Developmental Biology Animal. 23 (8), pp. 575-580. https://doi.org/10.1007/BF02620975

Cultivating our wild relations.

G - Articles in popular magazines and other technical publications

Fish, N. and Jones, M. G. K. 1987. Cultivating our wild relations. Potato World. 4, pp. 22-23.

Ploidy variation in Solanum brevidens plants regenerated from protoplasts using an improved culture system

A - Papers appearing in refereed journals

Nelson, R. S., Karp, A. and Bright, S. W. J. 1986. Ploidy variation in Solanum brevidens plants regenerated from protoplasts using an improved culture system. Journal of Experimental Botany. 37 (175), pp. 253-261. https://doi.org/10.1093/jxb/37.2.253

Growth responses of Glomus caledonium in the presence of suspension cultured lucerne cells.

G - Articles in popular magazines and other technical publications

Carr, G. R., Hinkley, M. A., Le Tacon, F., Hepper, C. M., Jones, M. G. K. and Thomas, E. 1986. Growth responses of Glomus caledonium in the presence of suspension cultured lucerne cells.

Altered feedback sensitivity of acetohydroxyacid synthase from valine-resistant mutants of tobacco ( Nicotiana tabacum L.).

A - Papers appearing in refereed journals

Relton, J. M., Wallsgrove, R. M., Bourgin, J. P. and Bright, S. W. J. 1986. Altered feedback sensitivity of acetohydroxyacid synthase from valine-resistant mutants of tobacco ( Nicotiana tabacum L.). Planta. 169, pp. 46-50.

Mutation and tissue culture.

G - Articles in popular magazines and other technical publications

Bright, S. W. J., Ooms, G., Foulger, D., Karp, A. and Evans, N. 1986. Mutation and tissue culture.

Effects of exogenous amino acids on growth and activity of four aspartate pathway enzymes in barley.

A - Papers appearing in refereed journals

Rognes, S. E., Wallsgrove, R. M., Kueh, J. S. H. and Bright, S. W. J. 1986. Effects of exogenous amino acids on growth and activity of four aspartate pathway enzymes in barley. Plant Science. 43, pp. 45-50.

Biochemical characterisation of an auxotroph of Datura innoxia requiring isoleucine and valine.

A - Papers appearing in refereed journals

Wallsgrove, R. M., Risiott, R., King, J. and Bright, S. W. J. 1986. Biochemical characterisation of an auxotroph of Datura innoxia requiring isoleucine and valine. Plant Science. 43, pp. 109-114.

Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars.

A - Papers appearing in refereed journals

Foulger, D. and Jones, M. G. K. 1986. Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars. Plant Cell Reports. 5, pp. 72-76.

Biotechnology's potential for crop improvement.

G - Articles in popular magazines and other technical publications

Bright, S. W. J., Jones, M. G. K., Ooms, G., Maddock, S. E., Karp, A., Foulger, D. and Miflin, B. J. 1986. Biotechnology's potential for crop improvement.

Developments in the culture of plant protoplasts and cells and their regeneration to plants.

G - Articles in popular magazines and other technical publications

Jones, M. G. K. 1986. Developments in the culture of plant protoplasts and cells and their regeneration to plants.

Genetic analysis of photorespiratory mutants in barley.

G - Articles in popular magazines and other technical publications

Turner, J. C., Hall, N. P., Kendall, A. C., Wallsgrove, R. M. and Bright, S. W. J. 1986. Genetic analysis of photorespiratory mutants in barley.

Transient expression of CAT in wheat protoplasts.

G - Articles in popular magazines and other technical publications

Jones, M. G. K., De Both, M. T. J., Wu, Q. S., Sparks, C. A., Maddock, S. E. and Gleddie, S. 1986. Transient expression of CAT in wheat protoplasts.

Protoplast fusion of Solanum species.

G - Articles in popular magazines and other technical publications

Foulger, D., Fish, N., Karp, A., Bains, A., Cooper-Bland, S., Bright, S. W. J. and Jones, M. G. K. 1986. Protoplast fusion of Solanum species.

Direct gene transfer in Nicotiana protoplasts by electroporation of the neomycin phosphotransferase gene.

G - Articles in popular magazines and other technical publications

De Both, M. T. J. and Jones, M. G. K. 1986. Direct gene transfer in Nicotiana protoplasts by electroporation of the neomycin phosphotransferase gene.

Somaclonal variation in explant-derived potato clones over three tuber generations.

A - Papers appearing in refereed journals

Evans, N. E., Foulger, D., Farrer, L. and Bright, S. W. J. 1986. Somaclonal variation in explant-derived potato clones over three tuber generations. Euphytica. 35, pp. 353-361.

Lectin levels in tissues of cultured immature wheat embryos.

A - Papers appearing in refereed journals

Morris, P., Maddock, S. E., Jones, M. G. K. and Bowles, D. J. 1986. Lectin levels in tissues of cultured immature wheat embryos. Plant Cell Reports. 5, pp. 460-463.

Plant tissue culture technology and crop improvement

B - Book chapters etc edited externally

Jones, M. G. K. and Karp, A. 1985. Plant tissue culture technology and crop improvement. in: Mizrahi, A. and van Wezel, A. L. (ed.) Advances in Biotechnological Processes: Volume 5 New York Alan R. Liss, Inc.. pp. 91-121

Somaclonal variation in the gliadin patterns of grains of regenerated wheat plants

A - Papers appearing in refereed journals

Maddock, S. E., Risiott, R., Parmar, S., Jones, M. G. K. and Shewry, P. R. 1985. Somaclonal variation in the gliadin patterns of grains of regenerated wheat plants. Journal of Experimental Botany. 36 (173), pp. 1976-1984. https://doi.org/10.1093/jxb/36.12.1976

Regulation of storage protein-synthesis by sulfur and nitrogen nutrition in cultured immature caryopses of barley

A - Papers appearing in refereed journals

Bottacin, A., Smith, K., Miflin, B. J., Shewry, P. R. and Bright, S. W. J. 1985. Regulation of storage protein-synthesis by sulfur and nitrogen nutrition in cultured immature caryopses of barley. Journal of Experimental Botany. 36 (162), pp. 140-148. https://doi.org/10.1093/jxb/36.1.140

Plant genetics - transformation of cereal crops by direct gene-transfer

A - Papers appearing in refereed journals

Jones, M. G. K. 1985. Plant genetics - transformation of cereal crops by direct gene-transfer. Nature. 317 (6038), pp. 579-580. https://doi.org/10.1038/317579a0

A conditional-lethal molybdopterin-defective mutant of barley

A - Papers appearing in refereed journals

Wray, J. L., Steven, B., Kirk, D. W. and Bright, S. W. J. 1985. A conditional-lethal molybdopterin-defective mutant of barley. Molecular & General Genetics. 201 (3), pp. 462-466. https://doi.org/10.1007/bf00331340

Changes in the levels of wheat-germ and barley-germ agglutinin during embryogenesis invivo, invitro and during germination

A - Papers appearing in refereed journals

Morris, P. C., Maddock, S. E., Jones, M. G. K. and Bowles, D. J. 1985. Changes in the levels of wheat-germ and barley-germ agglutinin during embryogenesis invivo, invitro and during germination. Planta. 166 (3), pp. 407-413. https://doi.org/10.1007/bf00401180

Shoot formation from explant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants

A - Papers appearing in refereed journals

Wheeler, V. A., Evans, N. E., Foulger, D., Webb, K. J., Karp, A., Franklin, J. and Bright, S. W. J. 1985. Shoot formation from explant cultures of fourteen potato cultivars and studies of the cytology and morphology of regenerated plants. Annals of Botany. 55 (3), pp. 309-320. https://doi.org/10.1093/oxfordjournals.aob.a086907

Agriculture Group Symposium. Potential Impact of Biotechnology upon Agriculture: Novel Ways of Manipulating Plants

A - Papers appearing in refereed journals

Miflin, B. J., Jones, M. G. K., Ooms, G., Karp, A., Maddock, S. E. and Bright, S. W. J. 1985. Agriculture Group Symposium. Potential Impact of Biotechnology upon Agriculture: Novel Ways of Manipulating Plants. Journal of the Science of Food and Agriculture. 36 (8), pp. 651-652. https://doi.org/10.1002/jsfa.2740360805

On the causes and origins of somaclonal variation

B - Book chapters etc edited externally

Karp, A. and Bright, S. W. J. 1985. On the causes and origins of somaclonal variation. in: Miflin, B. J. (ed.) Oxford Surveys of Plant Molecular and Cell Biology: Volume 2 Oxford Oxford University Press (OUP). pp. 199-234

Directed electrofusion between protoplasts with different responses in a mass fusion system

A - Papers appearing in refereed journals

Tempelaar, M. J. and Jones, M. G. K. 1985. Directed electrofusion between protoplasts with different responses in a mass fusion system. Plant Cell Reports. 4 (2), pp. 92-95. https://doi.org/10.1007/bf00269215

Fusion characteristics of plant protoplasts in electric fields

A - Papers appearing in refereed journals

Tempelaar, M. J. and Jones, M. G. K. 1985. Fusion characteristics of plant protoplasts in electric fields. Planta. 165, pp. 205-216.

Improving the amino acid content of cereal grains

C1 - Edited contributions to conferences/learned societies

Miflin, B. J., Kreis, M., Bright, S. W. J. and Shewry, P. R. 1985. Improving the amino acid content of cereal grains. Copping, L. G. and Rodgers, P. (ed.) Biotechnology and its applications to agriculture. BCPC Monograph No 32 . British Crop Protection Council (BCPC). pp. 71-78

Applications of genetic engineering to agriculture

B - Book chapters etc edited externally

Jones, M. G. K. 1985. Applications of genetic engineering to agriculture. in: Dodds, J . H. (ed.) Plant genetic engineering pp. 269-295

Improved hyphal growth of two species of vesicular-arbuscular mycorrhizal fungi in the presence of suspension-cultured plant cells

A - Papers appearing in refereed journals

Carr, G. R., Hinkley, M. A., Le Tacon, F., Hepper, C. M., Jones, M. G. K. and Thomas, E. 1985. Improved hyphal growth of two species of vesicular-arbuscular mycorrhizal fungi in the presence of suspension-cultured plant cells. New Phytologist. 101 (3), pp. 417-426. https://doi.org/10.1111/j.1469-8137.1985.tb02848.x

Approaches to improving the nutritional quality of barley seed proteins

C1 - Edited contributions to conferences/learned societies

Shewry, P. R., Bright, S. W. J., Burgess, S. R. and Miflin, B. J. 1984. Approaches to improving the nutritional quality of barley seed proteins.  Cereal grain protein improvement. Proceedings Final Research Co-ordination Meeting of the FAO/IAEA/GSF/SIDA Co-ordinated Research Programme on the Use of Nuclear Techniques for Cereal Grain Protein Improvement, Vienna, 1982. Vienna, International Atomic Energy Agency (IAEA). pp. 227-239

Regulation of aspartate kinase isoenzymes in barley mutants resistant to lysine plus threonine - construction and analysis of combinations of the Lt1a, Lt1b, and Lt2 mutant-genes

A - Papers appearing in refereed journals

Arruda, P., Bright, S. W. J., Kueh, J. S. H., Lea, P. J. and Rognes, S. E. 1984. Regulation of aspartate kinase isoenzymes in barley mutants resistant to lysine plus threonine - construction and analysis of combinations of the Lt1a, Lt1b, and Lt2 mutant-genes. Plant Physiology. 76 (2), pp. 442-446. https://doi.org/10.1104/pp.76.2.442

Proline biosynthesis in a proline-accumulating barley mutant

A - Papers appearing in refereed journals

Kueh, J. S. H., Hill, J. M., Smith, S. J. and Bright, S. W. J. 1984. Proline biosynthesis in a proline-accumulating barley mutant. Phytochemistry. 23 (10), pp. 2207-2210. https://doi.org/10.1016/s0031-9422(00)80521-x

Acetohydroxyacid synthase in a valine-resistant mutant of nicotiana tabacum

A - Papers appearing in refereed journals

Wallsgrove, R. M., Bright, S. W. J. and Bourgin, J. P. 1984. Acetohydroxyacid synthase in a valine-resistant mutant of nicotiana tabacum. Plant Physiology. 75, pp. 35-35.

Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants

A - Papers appearing in refereed journals

Karp, A., Risiott, R., Jones, M. G. K. and Bright, S. W. J. 1984. Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants. Plant Cell, Tissue and Organ Culture. 3 (4), pp. 363-373. https://doi.org/10.1007/bf00043089

Manipulation of key pathways in photorespiration and amino acid metabolism by mutation and selection

C1 - Edited contributions to conferences/learned societies

Bright, S. W. J., Arruda, P., Hall, N. P., Kendall, A. C., Keys, A. J., Kueh, J. S. H., Parker, M. L., Rognes, S. E., Turner, J. C., Wallsgrove, R. M. and Miflin, B. J. 1984. Manipulation of key pathways in photorespiration and amino acid metabolism by mutation and selection. Lea, P. J. and Stewart, G. R. (ed.) Genetic manipulation of plants and its application to agriculture (Annual Proceedings of the Phytochemical Society of Europe, 23). Clarendon Press, Oxford. pp. 141-169

Plant-regeneration from protoplasts of solanum-brevidens

A - Papers appearing in refereed journals

Nelson, R. S., Creissen, G. P. and Bright, S. W. J. 1983. Plant-regeneration from protoplasts of solanum-brevidens. Plant science letters. 30 (3), pp. 355-362. https://doi.org/10.1016/0304-4211(83)90175-x

Lysine-sensitivity of aspartate kinase isoenzymes in barley plants with combinations of the lt1 and lt2 mutant genes

A - Papers appearing in refereed journals

Arruda, P., Bright, S. W. J., Kueh, J. S. H., Lea, P. J. and Rognes, S. E. 1983. Lysine-sensitivity of aspartate kinase isoenzymes in barley plants with combinations of the lt1 and lt2 mutant genes. Plant Physiology. 72, pp. 34-34.

Lysine transport in 2 barley mutants with altered uptake of basic-amino-acids in the root

A - Papers appearing in refereed journals

Bright, S. W. J., Kueh, J. S. H. and Rognes, S. E. 1983. Lysine transport in 2 barley mutants with altered uptake of basic-amino-acids in the root. Plant Physiology. 72 (3), pp. 821-824. https://doi.org/10.1104/pp.72.3.821

Improvement of protein-quality in cereals

A - Papers appearing in refereed journals

Bright, S. W. J. and Shewry, P. R. 1983. Improvement of protein-quality in cereals. Critical reviews in plant sciences. 1 (1), pp. 49-93. https://doi.org/10.1080/07352688309382171

Feedback-insensitive aspartate kinase isoenzymes in barley mutants resistant to lysine plus threonine

A - Papers appearing in refereed journals

Rognes, S. E., Bright, S. W. J. and Miflin, B. J. 1983. Feedback-insensitive aspartate kinase isoenzymes in barley mutants resistant to lysine plus threonine. Planta. 157 (1), pp. 32-38. https://doi.org/10.1007/bf00394537

A conditional-lethal cnx-type nitrate reductase-deficient barley mutant

A - Papers appearing in refereed journals

Bright, S. W. J., Norbury, P. B., Franklin, J., Kirk, D. W. and Wray, J. L. 1983. A conditional-lethal cnx-type nitrate reductase-deficient barley mutant. Molecular & General Genetics. 189 (2), pp. 240-244. https://doi.org/10.1007/bf00337811

The regeneration of plants from protoplasts of agriculturally important species [Triticum aestivum, wheat, Hordeum vulgare, barley, Solanum tuberosum potatoes, Brassica napus, rape]

A - Papers appearing in refereed journals

Thomas, E., Miflin, B. J., Bright, S. W. J. and Lancaster, V. 1982. The regeneration of plants from protoplasts of agriculturally important species [Triticum aestivum, wheat, Hordeum vulgare, barley, Solanum tuberosum potatoes, Brassica napus, rape]. The regeneration of plants from protoplasts of agriculturally important species.

Variation amongst protoplast-derived potato plants (solanum-tuberosum cv maris bard)

A - Papers appearing in refereed journals

Thomas, E., Bright, S. W. J., Franklin, J., Lancaster, V. A., Miflin, B. J. and Gibson, R. 1982. Variation amongst protoplast-derived potato plants (solanum-tuberosum cv maris bard). Theoretical and Applied Genetics. 62 (1), pp. 65-68. https://doi.org/10.1007/BF00276285

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