Visualisation of plastid degradation in sperm cells of wheat pollen

A - Papers appearing in refereed journals

Primavesi, L. F., Wu, H., Mudd, E. A., Day, A. and Jones, H. D. 2016. Visualisation of plastid degradation in sperm cells of wheat pollen. Protoplasma. 254 (1), pp. 229-237.

AuthorsPrimavesi, L. F., Wu, H., Mudd, E. A., Day, A. and Jones, H. D.
Abstract

Like most angiosperms, wheat (Triticum aestivum) shows maternal inheritance of plastids. It is thought that this takes place by cytoplasmic stripping at fertilisation rather than the absence of plastids in sperm cells. To determine the fate of plastids during sperm cell development, plastid-targeted green fluorescent protein was used to visualise these organelles in nuclear transgenic wheat lines. Fewer than thirty small 1–2-μm plastids were visible in early uninucleate pollen cells. These dramatically increased to several hundred larger (4 μm) plastids during pollen maturation and went through distinct morphological changes. Only small plastids were visible in generative cells (n = 25) and young sperm cells (n = 9). In mature sperm cells, these green fluorescent protein (GFP)-tagged plastids were absent. This is consistent with maternal inheritance of plastids resulting from their degradation in mature sperm cells in wheat.

Year of Publication2016
JournalProtoplasma
Journal citation254 (1), pp. 229-237
Digital Object Identifier (DOI)doi:10.1007/s00709-015-0935-x
Open accessPublished as non-open access
Funder project or codeWheat
Output statusPublished
Publication dates
Online21 Jan 2016
PublisherSpringer
Springer Wien
Copyright licensePublisher copyright
ISSN0033-183X

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