Expression of fission yeast cdc25 driven by the wheat ADP-glucose pyrophosphorylase large subunit promoter reduces pollen viability and prevents transmission of the transgene in wheat

A - Papers appearing in refereed journals

Chrimes, D., Rogers, H. J., Francis, D., Jones, H. D. and Ainsworth, C. 2005. Expression of fission yeast cdc25 driven by the wheat ADP-glucose pyrophosphorylase large subunit promoter reduces pollen viability and prevents transmission of the transgene in wheat. New Phytologist. 166 (1), pp. 185-192.

AuthorsChrimes, D., Rogers, H. J., Francis, D., Jones, H. D. and Ainsworth, C.
Abstract

Cell number was to be measured in wheat (Triticum aestivum) endosperm expressing Spcdc25 (a fission yeast cell-cycle regulator) controlled by a supposedly endosperm-specific promoter, AGP2 (from the large subunit of ADP glucose pyrophosphorylase). Wheat was transformed by biolistics either with AGP2::GUS or AGP2::Spcdc25. PCR and RT-PCR checked integration and expression of the transgene, respectively. In cv. Chinese Spring, AGP2::GUS was unexpectedly expressed in carpels and pollen, as well as endosperm. In cv. Cadenza, three AGP2::Spcdc25 plants, AGP2::Spcdc25.1, .2 and .3, were generated. Spcdc25 expression was detected in mature leaves of AGP2::Spcdc25.1/.3 which exhibited abnormal spikes, 50% pollen viability and low seed set per plant; both were small compared with the nonexpressing and normal AGP2::Spcdc25.2. Spcdc25 was not transmitted to the T-1 in AGP2::Spcdc25.1 or .3, which developed normally. Spcdc25 was PCR-positive in AGP2::Spcdc25.2, using primers for a central portion, but not with primers for the 5' end, of the ORF, indicating a rearrangement; Spcdc25 was not expressed in either T-0 or T-1. The AGP2 promoter is not tissue-specific and Spcdc25 expression disrupted reproduction.

KeywordsPlant Sciences
Year of Publication2005
JournalNew Phytologist
Journal citation166 (1), pp. 185-192
Digital Object Identifier (DOI)doi:10.1111/j.1469-8137.2004.01299.x
PubMed ID15760362
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or code501
PublisherWiley
Grant IDBBS/E/C/00004158
ISSN0028-646X

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