Development and application of cereal transformation technologies

Project (past)

Project dates01 Apr 1999 to end of 31 Mar 2008
FunderBiotechnology and Biological Sciences Research Council
Project number4158
DepartmentCrop Performance and Improvement (CPI) Division
Plant Sciences (PSC)
Research fish codeBBS/E/C/00004158
Grant IDBBS/E/C/00004158

Outputs

Sort by: Date Title

Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability

A - Papers appearing in refereed journals
Palomo-Rios, E., Macalpine, W. J., Shield, I. F., Amey, J. S., Karaoglu, C., West, J., Hanley, S. J., Krygier, R., Karp, A. and Jones, H. D. 2015. Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability. Canadian Journal Of Forest Research. 45 (11), pp. 1662-1667. https://doi.org/10.1139/cjfr-2015-0055

Manipulation of the cell wall composition of wheat endosperm cell walls to improve nutritional properties

C1 - Edited contributions to conferences/learned societies
Nemeth, C., Freeman, J., Mitchell, R. A. C. and Shewry, P. R. 2008. Manipulation of the cell wall composition of wheat endosperm cell walls to improve nutritional properties. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 295-297

A transgenic approach to understanding gene expression in cereals

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Freeman, J. and Sparks, C. A. 2008. A transgenic approach to understanding gene expression in cereals. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 427-430

Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes

A - Papers appearing in refereed journals
Wu, H., Doherty, A. and Jones, H. D. 2008. Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes. Transgenic Research. 17, pp. 425-436. https://doi.org/10.1007/s11248-007-9116-9

Technological quality of field grown transgenic lines of commercial wheat cultivars expressing the 1Ax1 HMW glutenin subunit gene

A - Papers appearing in refereed journals
Rakszegi, M., Pastori, G. M., Jones, H. D., Bekes, F., Butow, B., Lang, L., Bedo, Z. and Shewry, P. R. 2008. Technological quality of field grown transgenic lines of commercial wheat cultivars expressing the 1Ax1 HMW glutenin subunit gene. Journal of Cereal Science. 47, pp. 310-321. https://doi.org/10.1016/j.jcs.2007.04.010

Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins

A - Papers appearing in refereed journals
Primavesi, L. F., Wu, H., Mudd, E. A., Day, A. and Jones, H. D. 2008. Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins. Transgenic Research. 17, pp. 529-543. https://doi.org/10.1007/s11248-007-9126-7

Decreased shoot stature and grain alpha-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat

A - Papers appearing in refereed journals
Appleford, N. E., Wilkinson, M. D., Ma, Q., Evans, D. J., Stone, M. C., Pearce, S. P., Powers, S. J., Thomas, S. G., Jones, H. D., Phillips, A. L., Hedden, P. and Lenton, J. R. 2007. Decreased shoot stature and grain alpha-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat. Journal of Experimental Botany. 58, pp. 3213-3226. https://doi.org/10.1093/jxb/erm166

The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations

A - Papers appearing in refereed journals
Pastori, G. M., Huttly, A. K., West, J., Sparks, C. A., Pieters, A., Luna, C. M., Jones, H. D. and Foyer, C. H. 2007. The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations. Functional Plant Biology. 34 (9), pp. 835-843. https://doi.org/10.1071/FP07112

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance

A - Papers appearing in refereed journals
Yang, Y., Ma, Y. Z., Xu, Z. S., Chen, X. M., He, Z. H., Yu, Z., Wilkinson, M. D., Jones, H. D., Shewry, P. R. and Xia, L-Q. 2007. Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Journal of Experimental Botany. 58, pp. 2863-2871. https://doi.org/10.1093/jxb/erm073

High throughput Agrobacterium transformation of wheat: a tool for functional genomics

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wilkinson, M. D., Doherty, A. and Wu, H. 2007. High throughput Agrobacterium transformation of wheat: a tool for functional genomics. Buck, H. T., Salomon, N. and Nisi, J. E. (ed.) Wheat Production in Stressed Environments: Proceedings 7th International Wheat Conference, Mar del Plata, Argentina, 27 November-2 December 2005 . Springer, Dordrecht. pp. 693-699

The structure and expression of cereal storage protein genes

B - Book chapters etc edited externally
Halford, N. G. and Shewry, P. R. 2007. The structure and expression of cereal storage protein genes. in: Olsen, O-A. (ed.) Endosperm: developmental and molecular biology. (Plant Cell Monographs, Vol. 8) Springer, Berlin. pp. 195-218

Simple integration patterns observed by transformation with the Glu-D1x5 gene as a linear fragment

C1 - Edited contributions to conferences/learned societies
Yao, Q., Cong, L., He, G. Y., Chang, J. L., Li, K. X., Yang, G. X., Jones, H. D. and Shewry, P. R. 2007. Simple integration patterns observed by transformation with the Glu-D1x5 gene as a linear fragment. Ng, P. K. W. and Lookhart, G. L. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 6-8

Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins

C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Tosi, P., Jones, H. D., Sparks, C. A., Gritsch, C., Funatsuki, W., Niwa, K., Huttly, A. K., Napier, J. A., D'ovidio, R. and Freeman, J. 2007. Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. Lookhart, G. L. and Ng, P. W. K. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 112-116

Combined expression of gluten strength and grain hardness genes by crossing transgenic plants with Elite wheat varieties

C1 - Edited contributions to conferences/learned societies
Zhang, J. U., Li, K., Yang, G., Luo, L., Jones, H. D., Shewry, P. R. and He, G. 2007. Combined expression of gluten strength and grain hardness genes by crossing transgenic plants with Elite wheat varieties. Ng, P. K. W. and Lookhart, G. L. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 58-61

Expression of sequences encoding LMW subunits of glutenin

C1 - Edited contributions to conferences/learned societies
Lambourne, J. J., Tosi, P., Frazier, R. A., Bhandari, D. G. and Shewry, P. R. 2007. Expression of sequences encoding LMW subunits of glutenin. Ng, P. K. W. and Lookhart, G. L. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 154-157

Developing allergen-free foods by genetic manipulation

B - Book chapters etc edited externally
Shewry, P. R. and Jones, H. D. 2007. Developing allergen-free foods by genetic manipulation. in: Mills, C., Hoffmann-Sommergruber, K. and Wichers, H. (ed.) Managing allergens in food Woodhead Publishing, Cambridge. pp. 147-158

Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation

A - Papers appearing in refereed journals
Wu, H., Sparks, C. A. and Jones, H. D. 2006. Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation. Molecular Breeding. 18, pp. 195-208. https://doi.org/10.1007/s11032-006-9027-0

Transformationand expression of high molecular weight glutenin subunit genes in common wheat (Triticum aestivum L.) varieties

A - Papers appearing in refereed journals
Shi, N-N., He, G-Y., Li, K-X., Jones, H. D. and Shewry, P. R. 2006. Transformationand expression of high molecular weight glutenin subunit genes in common wheat (Triticum aestivum L.) varieties. Scientia Agricultura Sinica. 38, pp. 874-881.

Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes

A - Papers appearing in refereed journals
Shewry, P. R., Powers, S. J., Field, J. M., Fido, R. J., Jones, H. D., Arnold, G. M., West, J., Lazzeri, P. A., Barcelo, P., Barro, F., Tatham, A. S., Bekes, F., Butow, B. and Darlington, H. 2006. Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes. Theoretical and Applied Genetics. 113, pp. 128-136. https://doi.org/10.1007/s00122-006-0279-1

Advances in transformation technologies

B - Book chapters etc edited externally
Jones, H. D. 2006. Advances in transformation technologies. in: Halford, N. G. (ed.) Plant biotechnology: current and future uses of genetically modified crops John Wiley & Sons Chichester, West Sussex. pp. 71-90

Transgenic wheat: where do we stand after the first 12 years?

A - Papers appearing in refereed journals
Shewry, P. R. and Jones, H. D. 2005. Transgenic wheat: where do we stand after the first 12 years? Annals of Applied Biology - AAB. 147 (1), pp. 1-14. https://doi.org/10.1111/j.1744-7348.2005.00009.x

Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat

A - Papers appearing in refereed journals
Jones, H. D., Doherty, A. and Wu, H. 2005. Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat. Plant Methods. 1:5. https://doi.org/10.1186/1746-4811-1-5

Investigating plastid development and gene flow using plastid-targeted GFP in transgenic wheat

C2 - Non-edited contributions to conferences
Primavesi, L. F., Wu, H., Mudd, E. A., Day, A. and Jones, H. D. 2005. Investigating plastid development and gene flow using plastid-targeted GFP in transgenic wheat.

Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25

A - Papers appearing in refereed journals
Howarth, J. R., Jacquet, J. N., Doherty, A., Jones, H. D. and Cannell, M. E. 2005. Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25. Annals of Applied Biology - AAB. 146 (3), pp. 311-320. https://doi.org/10.1111/j.1744-7348.2005.040121.x

Expression of fission yeast cdc25 driven by the wheat ADP-glucose pyrophosphorylase large subunit promoter reduces pollen viability and prevents transmission of the transgene in wheat

A - Papers appearing in refereed journals
Chrimes, D., Rogers, H. J., Francis, D., Jones, H. D. and Ainsworth, C. 2005. Expression of fission yeast cdc25 driven by the wheat ADP-glucose pyrophosphorylase large subunit promoter reduces pollen viability and prevents transmission of the transgene in wheat. New Phytologist. 166 (1), pp. 185-192. https://doi.org/10.1111/j.1469-8137.2004.01299.x

Wheat transformation: current technology and applications to grain development and composition

A - Papers appearing in refereed journals
Jones, H. D. 2005. Wheat transformation: current technology and applications to grain development and composition. Journal of Cereal Science. 41, pp. 137-147.

The molecular basis for grain texture in wheat

C1 - Edited contributions to conferences/learned societies
Wiley, P. R., Tosi, P., Jones, H. D. and Shewry, P. R. 2004. The molecular basis for grain texture in wheat. Fischer, T. (ed.) New Directions for a Diverse Planet: Proceedings 4th International Crop Science Congress, Brisbane, 26 September-1 October 2002 . Regional Institute, Gosford. pp. www.cropscience.org.au

Transformation of wheat by biolistics

B - Book chapters etc edited externally
Sparks, C. A. and Jones, H. D. 2004. Transformation of wheat by biolistics. in: Curtis, I. S. (ed.) Transgenic crops of the world: essential protocols Kluwer Academic Publishers, Dordrecht, Netherlands. pp. 19-33

Structure and biomechanical properties of the gluten proteins deposited in the developing wheat grain: relationship to functional properties at harvest

C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Wellner, N., Mills, E. N. C., Brownsey, G., Wilson, R. H., Brown, N. A., Freeman, J., Halford, N. G. and Belton, P. S. 2004. Structure and biomechanical properties of the gluten proteins deposited in the developing wheat grain: relationship to functional properties at harvest. Black, C. K., Rebetzke, G. J. and Panozzo, J. F. (ed.) Cereals 2004: Proceedings 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders' Assembly, Canberra, 21-24 September 2004 . Royal Australian Chemical Institute, Melbourne. pp. 377-379

Wheat transgenics: where are we now?

C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Pastori, G. M., Bekes, F. and Jones, H. D. 2004. Wheat transgenics: where are we now? Black, C. K., Rebetzke, G. J. and Panozzo, J. F. (ed.) Cereals 2004: Proceedings 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders' Assembly, Canberra, 21-24 September 2004 . Royal Australian Chemical Institute, Melbourne. pp. 380-382

Can biotechnology and genomics offer better routes to crop protection?

G - Articles in popular magazines and other technical publications
Jones, H. D. and Kanyuka, K. 2004. Can biotechnology and genomics offer better routes to crop protection? Outlooks on Pest Management. 15, pp. 217-221.

Selectable markers: antibiotic and herbicide resistance

B - Book chapters etc edited externally
Goodwin, J. L., Pastori, G. M., Davey, M. R. and Jones, H. D. 2004. Selectable markers: antibiotic and herbicide resistance. in: Pena, L. (ed.) Transgenic plants: methods and protocols (Methods in molecular biology 286) Humana Press, Totowa. pp. 191-201

Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

A - Papers appearing in refereed journals
Mendoza De Gyves, E., Sparks, C. A., Sayanova, O. V., Lazzeri, P. A., Napier, J. A. and Jones, H. D. 2004. Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.). Plant Biotechnology Journal. 2 (4), pp. 351-357. https://doi.org/10.1111/j.1467-7652.2004.00079.x

The use of biotechnology to study wheat endosperm development and improve grain quality

C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Jones, H. D., Holdsworth, M. J., Lenton, J. R. and Edwards, K. J. 2004. The use of biotechnology to study wheat endosperm development and improve grain quality. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 Septmeber 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 3-5

The potential of biotechnology to produce novel gluten phenotypes

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A., Doherty, A., Cannell, M., Goodwin, J., Pastori, G. M. and Shewry, P. R. 2004. The potential of biotechnology to produce novel gluten phenotypes. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 September 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 14-17

Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat

C2 - Non-edited contributions to conferences
Doherty, A., West, J. M., Sparks, C. A., Stone, M. C., Cannell, M. and Jones, H. D. 2003. Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .

CLAVATA1 dominant-negative alleles reveal functional overlap between multiple receptor kinases that regulate meristem and organ development

A - Papers appearing in refereed journals
Dievert, A., Dalal, M., Tax, F. E., Lacey, A. D., Huttly, A. K., Li, J. and Clark, S. E. 2003. CLAVATA1 dominant-negative alleles reveal functional overlap between multiple receptor kinases that regulate meristem and organ development. The Plant Cell. 15 (5), pp. 1198-1211. https://doi.org/10.​1105/​tpc.​010504