Expression of the gamma-zein protein of maize in seeds of transgenic barley: effects on grain composition and properties

A - Papers appearing in refereed journals

Zhang, Y., Darlington, H., Jones, H. D., Halford, N. G., Napier, J. A., Davey, M. R., Lazzeri, P. A. and Shewry, P. R. 2003. Expression of the gamma-zein protein of maize in seeds of transgenic barley: effects on grain composition and properties. Theoretical and Applied Genetics. 106 (6), pp. 1139-1146.

AuthorsZhang, Y., Darlington, H., Jones, H. D., Halford, N. G., Napier, J. A., Davey, M. R., Lazzeri, P. A. and Shewry, P. R.
Abstract

A cDNA clone encoding the gamma-zein protein of maize was expressed in developing grain of barley using the starchy endosperm cell-specific promoter from the wheat Glu-1D-1 (HMW subunit 1Dx5) gene. Seven transgenic lines were recovered from 226 bombarded immature embryos, of which two were sterile and four tetraploid, while five were shown to express the gamma-zein protein based on western blotting. Southern blot analysis showed the presence of between about three and twelve transgene insertions. Detailed comparative studies of five null and five homozygous transformed sub-lines from transgenic line A showed that gamma-zein accounted for over 4% of the total prolamin fraction, corresponding to about 1.9% of the total grain N. Comparison of the proteins present in the gel protein fraction demonstrated that the gamma-zein was incorporated into polymers, as in maize. However, there was no effect on grain hardness measured using the Perten Single Kernel Characterisation System or on the vitreousness measured by visual inspection. This contrasts with the situation in maize where a clear association with vitreousness has been reported.

KeywordsBarley; Transgenic plants; Grain texture; Grain vitreousness; g-Zein
Year of Publication2003
JournalTheoretical and Applied Genetics
Journal citation106 (6), pp. 1139-1146
Digital Object Identifier (DOI)doi:10.1007/s00122-002-1162-3
PubMed ID12671764
Open accessPublished as non-open access
Funder project or code415
503
Output statusPublished
Publication dates
Online28 Feb 2003
Publication process dates
Accepted06 Aug 2002
PublisherSpringer Nature
Springer
Copyright licensePublisher copyright
ISSN0040-5752

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