Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans

A - Papers appearing in refereed journals

West, A. L., Michaelson, L. V., Miles, E. A., Haslam, R. P., Lillycrop K. A., Georgescu, R., Han, L., Sayanova, O. V., Napier, J. A., Calder, P. C. and Burdge, G. C. 2020. Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1865 (8), p. 158710. https://doi.org/10.1016/j.bbalip.2020.158710

AuthorsWest, A. L., Michaelson, L. V., Miles, E. A., Haslam, R. P., Lillycrop K. A., Georgescu, R., Han, L., Sayanova, O. V., Napier, J. A., Calder, P. C. and Burdge, G. C.
Abstract

The mechanisms by which digested fat is absorbed and transported in the circulation are well documented. However, it is uncertain whether the molecular species composition of dietary fats influences the molecular species composition of meal-derived lipids in blood. This may be important because enzymes that remove meal-derived fatty acids from the circulation exhibit differential activities towards individual lipid molecular species. To determine the effect of consuming oils with different molecular compositions on the incorporation of 20:5n-3 and 22:6n-3 into plasma lipid molecular species. Men and women (18 - 30 years) consumed standardised meals containing 20:5n-5 and 22:6n-3 (total 450mg) provided by an oil from transgenic Camelina sativa (CSO) or a blended fish oil (BFO) which differed in the composition of 20:5n-3 and 22:6n-3 – containing molecular species. Blood was collected during the subsequent 8 hours. Samples were analysed by liquid chromatography-mass spectrometry. The molecular species composition of the test oils was distinct from the composition of plasma triacylglycerol (TG) or phosphatidylcholine (PC) molecular species at baseline and at 1.5 or 6 hours after the meal. The rank order by concentration of both plasma PC and TG molecular species at baseline was maintained during the postprandial period. 20:5n-3 and 22:6n-3 were incorporated preferentially into plasma PC compared to plasma TG. Together these findings suggest that the composition of dietary lipids undergoes extensive rearrangement after absorption, such that plasma TG and PC maintain their molecular species composition, which may facilitate lipase activities in blood and/or influence lipoprotein structural stability and function.

KeywordsPhosphatidylcholine; Triacylglycerol; Docosahexaenoic acid; Eicosapentaenoic acid; Postprandial; Molecular species
Year of Publication2020
JournalBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
Journal citation1865 (8), p. 158710
Digital Object Identifier (DOI)https://doi.org/10.1016/j.bbalip.2020.158710
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCan oils derived from genetically-modified plants replace fish oil as a source of long chain n-3 polyunsaturated fatty acids in the human diet?
Tailoring Plant Metabolism ISP
Output statusPublished
Publication dates
Online11 Apr 2020
Publication process dates
Accepted05 Apr 2020
PublisherElsevier
ISSN1388-1981

Permalink - https://repository.rothamsted.ac.uk/item/9772x/differential-postprandial-incorporation-of-20-5n-3-and-22-6n-3-into-individual-plasma-triacylglycerol-and-phosphatidylcholine-molecular-species-in-humans

Restricted files

Publisher's version

Under embargo indefinitely

Accepted author manuscript

Under embargo indefinitely

33 total views
2 total downloads
3 views this month
0 downloads this month