A - Papers appearing in refereed journals
Qi, B., Fraser, T., Mugford, S., Dobson, G., Sayanova, O. V., Butler, J., Napier, J. A., Stobart, A. K. and Lazarus, C. M. 2004. Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants. Nature Biotechnology. 22 (6), pp. 739-745.
|Authors||Qi, B., Fraser, T., Mugford, S., Dobson, G., Sayanova, O. V., Butler, J., Napier, J. A., Stobart, A. K. and Lazarus, C. M.|
We report the production of two very long chain polyunsaturated fatty acids, arachidonic acid (AA) and eicosapentaenoic acid (EPA), in substantial quantities in a higher plant. This was achieved using genes encoding enzymes participating in the ω3/6 Δ8-desaturation biosynthetic pathways for the formation of C20 polyunsaturated fatty acids. Arabidopsis thaliana was transformed sequentially with genes encoding a Δ9-specific elongating activity from Isochrysis galbana, a Δ8-desaturase from Euglena gracilis and a Δ5-desaturase from Mortierella alpina. Instrumental in the successful reconstitution of these C20 polyunsaturated fatty acid biosynthetic pathways was the I. galbana C18-Δ9-elongating activity, which may bypass rate-limiting steps present in the conventional Δ6-desaturase/elongase pathways. The accumulation of EPA and AA in transgenic plants is a breakthrough in the search for alternative sustainable sources of fish oils.
|Keywords||RRES175; 175_Plant sciences; 175_Genetics; 175_Nutrition|
|Year of Publication||2004|
|Journal citation||22 (6), pp. 739-745|
|Digital Object Identifier (DOI)||doi:10.1038/nbt972|
|Open access||Published as green open access|
|Funder project or code||503|
|Online||16 May 2004|
|Publication process dates|
|Accepted||19 Feb 2004|
|Copyright license||Publisher copyright|
Permalink - https://repository.rothamsted.ac.uk/item/89357/production-of-very-long-chain-polyunsaturated-omega-3-and-omega-6-fatty-acids-in-plants