A - Papers appearing in refereed journals
Han, L., Silvestre, S., Sayanova, O. V., Haslam, R. P. and Napier, J. A. 2022. Using field evaluation and systematic iteration to rationalize the accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa. Plant Biotechnology Journal. 20 (9), pp. 1833-1852. https://doi.org/10.1111/pbi.13867
Authors | Han, L., Silvestre, S., Sayanova, O. V., Haslam, R. P. and Napier, J. A. |
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Abstract | The Brassicaceae Camelina sativa (gold of pleasure) is now an established niche crop and being used as a transgenic host for a range of novel seed traits. Most notable of these is the accumulation of omega-3 long-chain polyunsaturates such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), fatty acids normally only found in marine organisms. As part of continued efforts to optimize the accumulation of these non-native fatty acids via seed-specific expression of algal genes, a new series of iterative constructs was built and introduced into Camelina. Seed fatty acid composition was determined, and the presence of EPA and DHA was confirmed. To provide an additional level of evaluation, full environmental release was carried out on selected events, providing a real-world gauntlet against which to assess the performance of these novel lines. Composition of the seed oil triacylglycerol was determined by mass spectrometry, allowing for conclusions as to the contribution of different activities to the final accumulation of EPA and DHA. Since these data were derived from field-grown material, they also represent a robust demonstration of the stability of the omega-3 LC-PUFA trait in Camelina. We propose that field trialling should be routinely incorporated in the plant synthetic biology 'design-build-test-learn' cycle. |
Keywords | Field Trials; GM Crops; Omega-3; Metabolic Engineering; Docosahexaenoic acid; Desaturases; Specificity; Elongases; Plants |
Year of Publication | 2022 |
Journal | Plant Biotechnology Journal |
Journal citation | 20 (9), pp. 1833-1852 |
Digital Object Identifier (DOI) | https://doi.org/10.1111/pbi.13867 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry |
Publisher's version | |
Output status | Published |
Publication dates | |
Online | 02 Jun 2022 |
Publication process dates | |
Accepted | 27 May 2022 |
Publisher | Wiley |
ISSN | 1467-7644 |
Permalink - https://repository.rothamsted.ac.uk/item/9899y/using-field-evaluation-and-systematic-iteration-to-rationalize-the-accumulation-of-omega-3-long-chain-polyunsaturated-fatty-acids-in-transgenic-camelina-sativa