Using field evaluation and systematic iteration to rationalize the accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa

A - Papers appearing in refereed journals

Han, L., Silvestre, S., Sayanova, O. V., Haslam, R. P. and Napier, J. A. 2022. Using field evaluation and systematic iteration to rationalize the accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa. Plant Biotechnology Journal. 20 (9), pp. 1833-1852. https://doi.org/10.1111/pbi.13867

AuthorsHan, L., Silvestre, S., Sayanova, O. V., Haslam, R. P. and Napier, J. A.
Abstract

The Brassicaceae Camelina sativa (gold of pleasure) is now an established niche crop and being used as a transgenic host for a range of novel seed traits. Most notable of these is the accumulation of omega-3 long-chain polyunsaturates such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), fatty acids normally only found in marine organisms. As part of continued efforts to optimize the accumulation of these non-native fatty acids via seed-specific expression of algal genes, a new series of iterative constructs was built and introduced into Camelina. Seed fatty acid composition was determined, and the presence of EPA and DHA was confirmed. To provide an additional level of evaluation, full environmental release was carried out on selected events, providing a real-world gauntlet against which to assess the performance of these novel lines. Composition of the seed oil triacylglycerol was determined by mass spectrometry, allowing for conclusions as to the contribution of different activities to the final accumulation of EPA and DHA. Since these data were derived from field-grown material, they also represent a robust demonstration of the stability of the omega-3 LC-PUFA trait in Camelina. We propose that field trialling should be routinely incorporated in the plant synthetic biology 'design-build-test-learn' cycle.

KeywordsField Trials; GM Crops; Omega-3; Metabolic Engineering; Docosahexaenoic acid; Desaturases; Specificity; Elongases; Plants
Year of Publication2022
JournalPlant Biotechnology Journal
Journal citation20 (9), pp. 1833-1852
Digital Object Identifier (DOI)https://doi.org/10.1111/pbi.13867
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeTPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Publisher's version
Output statusPublished
Publication dates
Online02 Jun 2022
Publication process dates
Accepted27 May 2022
PublisherWiley
ISSN1467-7644

Permalink - https://repository.rothamsted.ac.uk/item/9899y/using-field-evaluation-and-systematic-iteration-to-rationalize-the-accumulation-of-omega-3-long-chain-polyunsaturated-fatty-acids-in-transgenic-camelina-sativa

103 total views
67 total downloads
0 views this month
1 downloads this month
Download files as zip