Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry

Repository project

Project dates01 Apr 2017 to end of 31 Mar 2023
Researchers
Principal InvestigatorJohnathan Napier
Co-InvestigatorFreddie Theodoulou
FunderBiotechnology and Biological Sciences Research Council
Project numberI0420
I0400
DepartmentPlant Sciences
Research fish codeBBS/E/C/000I0420
Grant IDBBS/E/C/000I0420

Outputs

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Inclusion of oil from transgenic Camelina sativa in feed effectively supplies EPA and DHA to Atlantic salmon (Salmo salar) grown to market size in seawater pens

A - Papers appearing in refereed journals
Tocher, D. R., Sprague, M., Han, L., Sayanova, O. V., Norambuena, F., Napier, J. A. and Betancor, M. B. 2024. Inclusion of oil from transgenic Camelina sativa in feed effectively supplies EPA and DHA to Atlantic salmon (Salmo salar) grown to market size in seawater pens. Food Chemistry. 456, p. 139414. https://doi.org/10.1016/j.foodchem.2024.139414

BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition

A - Papers appearing in refereed journals
Zhang, H., Rundle, C., Winter, N., Miricescu, A., Mooney B. C., Bachmair, A., Graciet, E. and Theodoulou, F. L. 2024. BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition. The Plant Cell. p. koae117. https://doi.org/10.1093/plcell/koae117

A causal inference and Bayesian optimisation framework for modelling multi-trait relationships—Proof-of-concept using Brassica napus seed yield under controlled conditions

A - Papers appearing in refereed journals
Calderwood, A., Siles-Suarez, L., Eastmond, P. J., Kurup, S. and Morris, R. 2023. A causal inference and Bayesian optimisation framework for modelling multi-trait relationships—Proof-of-concept using Brassica napus seed yield under controlled conditions. PLOS ONE. 18 (9), p. 0290429. https://doi.org/10.1371/journal.pone.0290429

Chapter 11: The Properties, Distribution and Functionality of Cereal Lipids

B - Book chapters etc edited externally
Haslam, R. P. and Melis, S. 2023. Chapter 11: The Properties, Distribution and Functionality of Cereal Lipids. in: Shewry, P. R., Koshe, H. and Taylor, J. R. N (ed.) ICC Handbook of 21st Century Cereal Science and Technology Elsevier. pp. 103-110

Automated extraction of pod phenotype data from micro-computed tomograph

A - Papers appearing in refereed journals
Corcoran, E., Siles-Suarez, L., Kurup, S. and Ahnert, S. 2023. Automated extraction of pod phenotype data from micro-computed tomograph. Frontiers in Plant Science. 14, p. 1120182. https://doi.org/10.3389/fpls.2023.1120182

Tackling functional redundancy of Arabidopsis fatty acid elongase complexes

A - Papers appearing in refereed journals
Batsale, M., Alonso, M., Pascal, S., Thoraval, D., Haslam, R. P., Beaudoin, F., Domergue, F. and Joubes, J. 2023. Tackling functional redundancy of Arabidopsis fatty acid elongase complexes. Frontiers in Plant Science. 14, p. 1107333. https://doi.org/10.3389/fpls.2023.1107333

A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis

A - Papers appearing in refereed journals
Piovesana, M., Wood, A. K. M., Smith, D. P., Deery, M. J., Bayliss, R., Carrera, E., Napier, J. A., Kurup, S. and Matthes, M. C. 2023. A point mutation in the kinase domain of CRK10 leads to xylem vessel collapse and activation of defence responses in Arabidopsis. Journal of Experimental Botany. 74 (10), pp. 3104-3121. https://doi.org/10.1093/jxb/erad080

Relative Protein Lifetime Measurement in Plants Using Tandem Fluorescent Protein Timers

B - Book chapters etc edited externally
Zhang, H., Linster, E., Wirtz, M. and Theodoulou, F. L. 2022. Relative Protein Lifetime Measurement in Plants Using Tandem Fluorescent Protein Timers. in: Lois, L. M. and Trujillo, M. (ed.) Plant Proteostasis New York Humana Press Inc. pp. 201-220

A stable start - cotranslational Nt-acetylation promotes proteome stability across kingdoms

A - Papers appearing in refereed journals
Gibbs, D. J., Bailey, M. and Etherington, R. D. 2022. A stable start - cotranslational Nt-acetylation promotes proteome stability across kingdoms. Trends in Cell biology. 32 (5), pp. 374-376. https://doi.org/10.1016/j.tcb.2022.02.004

Something in the air

A - Papers appearing in refereed journals
Napier, J. A. 2022. Something in the air. Nature Sustainability. 5 (11), pp. 916-917. https://doi.org/10.1038/s41893-022-00954-0

Using field evaluation and systematic iteration to rationalize the accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa

A - Papers appearing in refereed journals
Han, L., Silvestre, S., Sayanova, O. V., Haslam, R. P. and Napier, J. A. 2022. Using field evaluation and systematic iteration to rationalize the accumulation of omega-3 long-chain polyunsaturated fatty acids in transgenic Camelina sativa. Plant Biotechnology Journal. 20 (9), pp. 1833-1852. https://doi.org/10.1111/pbi.13867

Enhancing the accumulation of eicosapentaenoic acid and docosahexaenoic acid in transgenic Camelina through the CRISPR-Cas9 inactivation of the competing FAE1 pathway

A - Papers appearing in refereed journals
Han, L., Haslam, R. P., Silvestre, S., Lu, C. and Napier, J. A. 2022. Enhancing the accumulation of eicosapentaenoic acid and docosahexaenoic acid in transgenic Camelina through the CRISPR-Cas9 inactivation of the competing FAE1 pathway. Plant Biotechnology Journal. 20 (8), pp. 1444-1446. https://doi.org/10.1111/pbi.13876

Plant proteostasis-a proven and promising target for crop improvement - Editorial

A - Papers appearing in refereed journals
Theodoulou, F. L., Orosa-Puente, B., Trujillo, M. and Rubio V. 2022. Plant proteostasis-a proven and promising target for crop improvement - Editorial. Essays in Biochemistry. 66 (2), pp. 75-85. https://doi.org/10.1042/EBC20210078

Ethylene augments root hypoxia tolerance via growth cessation and reactive oxygen species amelioration

A - Papers appearing in refereed journals
Liu, Z., Hartman, S., Van Veen, H., Zhang, H., Leeggangers , H. A. C. F., Martopawiro, S., Bosman, F., De Deugd, F., Su, P., Hummel, M., Rankenberg, T., Hassall, K. L., Bailey-Serres, J., Theodoulou, F. L., Voesenek L. A. C. J. and Sasidharan, R. 2022. Ethylene augments root hypoxia tolerance via growth cessation and reactive oxygen species amelioration. Plant Physiology. 190 (2), pp. 1365-1383. https://doi.org/10.1093/plphys/kiac245

Diverting phenylpropanoid pathway flux from sinapine to produce industrially useful 4-vinyl derivatives of hydroxycinnamic acids in Brassicaceous oilseeds

A - Papers appearing in refereed journals
Menard, G. N., Langdon, M., Bhunia, R., Shankhapal, A., Noleto-Dias, C., Lomax, C., Ward, J. L., Kurup, S. and Eastmond, P. J. 2022. Diverting phenylpropanoid pathway flux from sinapine to produce industrially useful 4-vinyl derivatives of hydroxycinnamic acids in Brassicaceous oilseeds. Metabolic Engineering. 70, pp. 196-205. https://doi.org/10.1016/j.ymben.2022.01.016

Ethylene augments root hypoxia tolerance through amelioration of reactive oxygen species and growth cessation

A - Papers appearing in refereed journals
Liu, Z., Hartman, S., Van Veen, H., Zhang, H., Leeggangers, H. A. C. F., Martopawiro, S., Bosman, F., De Deugd, F., Su, P., Hummel, M., Rankenberg, T., Hassall, K. L., Bailey-Serres, J., Theodoulou, F. L., Voesenek, L. A. C. J. and Sasidharan, R. 2022. Ethylene augments root hypoxia tolerance through amelioration of reactive oxygen species and growth cessation. bioRxiv. https://doi.org/10.1101/2022.01.21.477196

The Production of EPA and DHA in Transgenic Plants as a Sustainable, Environmentally Friendly Source of Omega-3 Fish Oils for Use in Novel Foods.

A - Papers appearing in refereed journals
Napier, J. A. 2021. The Production of EPA and DHA in Transgenic Plants as a Sustainable, Environmentally Friendly Source of Omega-3 Fish Oils for Use in Novel Foods. Journal Of The American Oil Chemists Society. 98 (S1), pp. 126-126. https://doi.org/10.1002/aocs.12529

Uncovering Trait Associations Resulting in Maximal Seed Yield in Winter and Spring Oilseed Rape

A - Papers appearing in refereed journals
Siles-Suarez, L., Hassall, K. L., Sanchis-Gritsch, C., Eastmond, P. J. and Kurup, S. 2021. Uncovering Trait Associations Resulting in Maximal Seed Yield in Winter and Spring Oilseed Rape. Frontiers in Plant Science. 12 (6 September), p. 697576. https://doi.org/10.3389/fpls.2021.697576

Dietary Supplementation with Transgenic Camelina sativa Oil Containing 20:5n-3 and 22:6n-3 or Fish Oil Induces Differential Changes in the Transcriptome of CD3+ T Lymphocytes

A - Papers appearing in refereed journals
West, A. L., Miles, E. A., Han, L., Lillycrop, K. A., Napier, J. A., Calder, P. C. and Burdge, G. C. 2021. Dietary Supplementation with Transgenic Camelina sativa Oil Containing 20:5n-3 and 22:6n-3 or Fish Oil Induces Differential Changes in the Transcriptome of CD3+ T Lymphocytes. Nutrients. 13 (9), p. 3116. https://doi.org/10.3390/nu13093116

Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae

B - Book chapters etc edited externally
Haslam, R. P. and Larson, T. R. 2021. Techniques for the Measurement of Molecular Species of Acyl-CoA in Plants and Microalgae. in: Bartels, D. and Dobermann, P. (ed.) Plant Lipids: Methods and Protocols, Methods in Molecular Biology Springer Nature. pp. 203-218

A Field Day for Gene-Edited Brassicas and Crop Improvement

A - Papers appearing in refereed journals
Napier, J. A. 2021. A Field Day for Gene-Edited Brassicas and Crop Improvement. The CRISPR Journal. 4 (3), pp. 307-312. https://doi.org/10.1089/crispr.2021.29130.kad

Plastidial acyl carrier protein Δ9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum

A - Papers appearing in refereed journals
Smith, R., Jouhet, J., Gandini, C., Nekrasov, V., Marechal, E., Napier, J. A. and Sayanova, O. V. 2021. Plastidial acyl carrier protein Δ9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum. Plant Journal. 106 (5), pp. 1247-1259. https://doi.org/10.1111/tpj.15231

Transgenic camelina oil is an effective source of eicosapentaenoic acid and docosahexaenoic acid in diets for farmed rainbow trout, in terms of growth, tissue fatty acid content, and fillet sensory properties

A - Papers appearing in refereed journals
Osmond, A. T. Y., Arts, M. T., Bazinet, R. P., Napier, J. A., Han, L. and Colombo, S. M. 2021. Transgenic camelina oil is an effective source of eicosapentaenoic acid and docosahexaenoic acid in diets for farmed rainbow trout, in terms of growth, tissue fatty acid content, and fillet sensory properties. Journal of the World Aquaculture Society. 54 (4), pp. 961-986. https://doi.org/10.1111/jwas.12788

Synaptotagmins at the endoplasmic reticulum–plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress

A - Papers appearing in refereed journals
Ruiz-Lopez, N., Perez-Sancho, J., Del Valle, A. E., Haslam, R. P., Vanneste, S., Catala, R., Perea-Resa, C., Van Damme, D., Garcia-Hernandez, S., Albert A., Vallarino, J., Lin, J., Friml, J., Macho, A. P., Salinas, J., Rosado, A., Napier, J. A., Amorim-Silva, V. and Botella, M. A. 2021. Synaptotagmins at the endoplasmic reticulum–plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. The Plant Cell. 33 (7), p. 2431–2453. https://doi.org/10.1093/plcell/koab122

Oil from transgenic Camelina sativa as a source of EPA and DHA in feed for European sea bass (Dicentrarchus labrax L.)

A - Papers appearing in refereed journals
Betancor, M. B., MacEwan, A., Sprague, M., Gong, X., Montero, D., Han, L., Napier, J. A., Norambuena, F., Izquierdo, M. and Tocher, D. R. 2021. Oil from transgenic Camelina sativa as a source of EPA and DHA in feed for European sea bass (Dicentrarchus labrax L.). Aquaculture. 530 (15 January), p. 735759. https://doi.org/10.1016/j.aquaculture.2020.735759

Defning lipids and T cell receptors involved in the intrinsic allergenicity of nut proteins

A - Papers appearing in refereed journals
Wang, R., Ghumra, A., Cochrane, S., Fairclough, L., Broughton, R., Michaelson, L. V., Beaudoin, F. and Alcocer, M. J. C. 2020. Defning lipids and T cell receptors involved in the intrinsic allergenicity of nut proteins. Clinical and Translational Allergy. 10, p. 54. https://doi.org/10.1186/s13601-020-00358-3

Lipidomic Analysis of Plasma from Healthy Men and Women Shows Phospholipid Class and Molecular Species Differences between Sexes

A - Papers appearing in refereed journals
West, A. L., Michaelson, L. V., Miles, E. A., Haslam, R. P., Lillycrop, K. A., Georgescu, R., Han, L., Napier, J. A., Calder, P. C. and Burdge, G. C. 2020. Lipidomic Analysis of Plasma from Healthy Men and Women Shows Phospholipid Class and Molecular Species Differences between Sexes. Lipids. https://doi.org/10.1002/lipd.12293

Agriculture can help aquaculture become greener

A - Papers appearing in refereed journals
Napier, J. A., Haslam, R. P., Olsen, R-E., Tocher, D. R. and Betancor, M. B. 2020. Agriculture can help aquaculture become greener. Nature Food. 1, pp. 680-683. https://doi.org/10.1038/s43016-020-00182-9

Overexpression of an endogenous type 2 diacylglycerol acyltransferase in the marine diatom Phaeodactylum tricornutum enhances lipid production and omega-3 long-chain polyunsaturated fatty acid content

A - Papers appearing in refereed journals
Haslam, R. P., Hamilton, M. L., Economou, C., Smith, Richard T., Hassall, K. L., Napier, J. A. and Sayanova, O. V. 2020. Overexpression of an endogenous type 2 diacylglycerol acyltransferase in the marine diatom Phaeodactylum tricornutum enhances lipid production and omega-3 long-chain polyunsaturated fatty acid content . Biotechnology for Biofuels. 13 (87). https://doi.org/10.1186/s13068-020-01726-8

Phytochrome-dependent temperature perception modulates isoprenoid metabolism

A - Papers appearing in refereed journals
Bianchetti, R. E., De Luca, B., De Haro, L. A., Rosado, D., Demarco, D., Conte, M., Bermudez, L, Freschi, L., Fernie, A. R., Michaelson, L. V., Haslam, R. P., Rossi, M. and Carrari, F. 2020. Phytochrome-dependent temperature perception modulates isoprenoid metabolism. Plant Physiology. 183 (3), pp. 869-882. https://doi.org/10.1104/pp.20.00019

High level accumulation of EPA and DHA in field-grown transgenic Camelina – a multi-territory evaluation of TAG accumulation and heterogeneity

A - Papers appearing in refereed journals
Han, L., Usher, S. L., Sandgrind, S., Hassall, K. L., Sayanova, O. V., Michaelson, L. V., Haslam, R. P. and Napier, J. A. 2020. High level accumulation of EPA and DHA in field-grown transgenic Camelina – a multi-territory evaluation of TAG accumulation and heterogeneity . Plant Biotechnology Journal. 18 (11), pp. 2280-2291. https://doi.org/10.1111/pbi.13385

Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans

A - Papers appearing in refereed journals
West, A. L., Michaelson, L. V., Miles, E. A., Haslam, R. P., Lillycrop K. A., Georgescu, R., Han, L., Sayanova, O. V., Napier, J. A., Calder, P. C. and Burdge, G. C. 2020. Differential postprandial incorporation of 20:5n-3 and 22:6n-3 into individual plasma triacylglycerol and phosphatidylcholine molecular species in humans. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1865 (8), p. 158710. https://doi.org/10.1016/j.bbalip.2020.158710

Impairment of DHA synthesis alters the expression of neuronal plasticity markers and the brain inflammatory status in mice

A - Papers appearing in refereed journals
Talamonti, E., Sasso, V., To, H., Haslam, R. P., Napier, J. A., Ulfhake, B., Pernold, K., Asadi, A., Hessa, T., Jacobsson, A., Chiurchiu, V. and Viscomi, M. T. 2020. Impairment of DHA synthesis alters the expression of neuronal plasticity markers and the brain inflammatory status in mice. The FASEB Journal. 34 (2), pp. 2024-2040. https://doi.org/10.1096/fj.201901890RR

DES2 is a fatty acid Delta 11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis

A - Papers appearing in refereed journals
Cheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J. 2020. DES2 is a fatty acid Delta 11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis. FEBS Letters. https://doi.org/10.1002/1873-3468.13762

Identification of a Δ11 desaturase from the arbuscular mycorrhizal fungus Rhizophagus irregularis

A - Papers appearing in refereed journals
Cheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J. 2020. Identification of a Δ11 desaturase from the arbuscular mycorrhizal fungus Rhizophagus irregularis. bioRxiv. https://doi.org/10.1101/2020.01.13.903815

Metabolic Engineering and Synthetic Biology Approaches to Enhancing Production of Long-Chain Polyunsaturated Fatty acids in Microalgae

B - Book chapters etc edited externally
Khozin-Goldberg, I. and Sayanova, O. V. 2020. Metabolic Engineering and Synthetic Biology Approaches to Enhancing Production of Long-Chain Polyunsaturated Fatty acids in Microalgae. in: Hallmann, A. and Rampelotto, P. (ed.) Grand Challenges in Algae Biotechnology. Grand Challenges in Biology and Biotechnology. Springer. pp. 249-289

Nutritional enhancement in plants – green and greener

A - Papers appearing in refereed journals
Napier, J. A. and Sayanova, O. V. 2020. Nutritional enhancement in plants – green and greener. Current Opinion in Biotechnology. 61 (February), pp. 122-127. https://doi.org/10.1016/j.copbio.2019.12.010

The Arabidopsis thaliana N-recognin E3 ligase PROTEOLYSIS1 influences the immune response.

A - Papers appearing in refereed journals
Till, C. J., Vicente, J., Zhang, H., Oszvald, M., Deery, M. J., Pastor, V., Lilley, K. S., Ray, R. V., Theodoulou, F. L. and Holdsworth, M. J. 2019. The Arabidopsis thaliana N-recognin E3 ligase PROTEOLYSIS1 influences the immune response. Plant Direct. 3 (12), p. e00194. https://doi.org/10.1002/pld3.194

Characterisation of Oil Palm Acyl-CoA-Binding Proteins and Correlation of their Gene Expression with Oil Synthesis

A - Papers appearing in refereed journals
Amiruddin, N., Chan, P-K., Azizi, N., Morris, P. E., Cham, K-L., Ong, P. W., Rosli, R., Masura, S. S., Murphy, D. J., Sambanthamurthi, R., Haslam, R. P., Chye, M-L., Harwood, J. L. and Low, E-T. L. 2019. Characterisation of Oil Palm Acyl-CoA-Binding Proteins and Correlation of their Gene Expression with Oil Synthesis. Plant and cell physiology. 61, pp. 735-747. https://doi.org/10.1093/pcp/pcz237

Arabidopsis cytosolic acyl-CoA-binding proteins function in determining seed oil composition

A - Papers appearing in refereed journals
Guo, Z. H., Ye, Z. W., Haslam, R. P., Michaelson, L. V., Napier, J. A. and Chye, M. L. 2019. Arabidopsis cytosolic acyl-CoA-binding proteins function in determining seed oil composition. Plant Direct. 3 (12), p. e00182. https://doi.org/10.1002/pld3.182