Identification of a Δ11 desaturase from the arbuscular mycorrhizal fungus Rhizophagus irregularis

A - Papers appearing in refereed journals

Cheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J. 2020. Identification of a Δ11 desaturase from the arbuscular mycorrhizal fungus Rhizophagus irregularis. bioRxiv.

AuthorsCheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J.
Abstract

Arbuscular mycorrhizal fungi are oleaginous organisms and the most abundant fatty acyl moiety usually found in their lipids is palmitvaccenic acid (16:1Δ11cis). However, it is not known how this uncommon fatty acid species is made. Here we have cloned two homologs of Lepidopteran fatty acyl-CoenzymeA Δ11 desaturases from Rhizophagus irregularis. Both DES1 and DES2 are expressed in intraradicle mycelium and can complement the unsaturated fatty acid-requiring auxotrophic growth phenotype of the Saccharomyces cerevisiae ole1Δ mutant. DES1 expression leads almost exclusively to oleic acid (18:1Δ9cis) production, whereas DES2 expression results in the production of 16:1Δ11cis and vaccenic acid (18:1Δ11cis). DES2 therefore encodes a Δ11 desaturase that is likely to be responsible for the synthesis of 16:1Δ11cis in R. irregularis.

KeywordsPalmitvaccenic acid; Fatty acid desaturase; Arbuscular mycorrhizal fungi; Rhizophagus irregulus
Year of Publication2020
JournalbioRxiv
Digital Object Identifier (DOI)doi:10.1101/2020.01.13.903815
Web address (URL)http://dx.doi.org/10.1101/2020.01.13.903815
Open accessPublished as non-open access
Funder project or codeTPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Output statusPublished
Publication dates
Online13 Jan 2020
PublisherPublic Library of Science (PLOS)

Permalink - https://repository.rothamsted.ac.uk/item/974qz/identification-of-a-11-desaturase-from-the-arbuscular-mycorrhizal-fungus-rhizophagus-irregularis

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