A - Papers appearing in refereed journals
Cheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J. 2020. Identification of a Δ11 desaturase from the arbuscular mycorrhizal fungus Rhizophagus irregularis. bioRxiv. https://doi.org/10.1101/2020.01.13.903815
Authors | Cheeld, H., Bhutada, G., Beaudoin, F. and Eastmond, P. J. |
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Abstract | Arbuscular mycorrhizal fungi are oleaginous organisms and the most abundant fatty acyl moiety usually found in their lipids is palmitvaccenic acid (16:1Δ11cis). However, it is not known how this uncommon fatty acid species is made. Here we have cloned two homologs of Lepidopteran fatty acyl-CoenzymeA Δ11 desaturases from Rhizophagus irregularis. Both DES1 and DES2 are expressed in intraradicle mycelium and can complement the unsaturated fatty acid-requiring auxotrophic growth phenotype of the Saccharomyces cerevisiae ole1Δ mutant. DES1 expression leads almost exclusively to oleic acid (18:1Δ9cis) production, whereas DES2 expression results in the production of 16:1Δ11cis and vaccenic acid (18:1Δ11cis). DES2 therefore encodes a Δ11 desaturase that is likely to be responsible for the synthesis of 16:1Δ11cis in R. irregularis. |
Keywords | Palmitvaccenic acid; Fatty acid desaturase; Arbuscular mycorrhizal fungi; Rhizophagus irregulus |
Year of Publication | 2020 |
Journal | bioRxiv |
Digital Object Identifier (DOI) | https://doi.org/10.1101/2020.01.13.903815 |
Web address (URL) | http://dx.doi.org/10.1101/2020.01.13.903815 |
Open access | Published as non-open access |
Funder project or code | Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry |
Output status | Published |
Publication dates | |
Online | 13 Jan 2020 |
Publisher | Public Library of Science (PLOS) |
Permalink - https://repository.rothamsted.ac.uk/item/974qz/identification-of-a-11-desaturase-from-the-arbuscular-mycorrhizal-fungus-rhizophagus-irregularis
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