Genomics of predictive radiation mutagenesis in oilseed rape: modifying seed oil composition.

A - Papers appearing in refereed journals

Havlickova, L., He, Z., Berger, M., Wang, L., Sandmann, G., Chew, Y. P., Yoshikawa, G. V., Lu, G., Hu, Q., Banga, S. S., Beaudoin, F. and Bancroft, I. 2023. Genomics of predictive radiation mutagenesis in oilseed rape: modifying seed oil composition. Plant Biotechnology Journal. pp. 1-13. https://doi.org/10.1111/pbi.14220

AuthorsHavlickova, L., He, Z., Berger, M., Wang, L., Sandmann, G., Chew, Y. P., Yoshikawa, G. V., Lu, G., Hu, Q., Banga, S. S., Beaudoin, F. and Bancroft, I.
Abstract

Rapeseed is a crop of global importance but there is a need to broaden the genetic diversity available to address breeding objectives. Radiation mutagenesis, supported by genomics, has the potential to supersede genome editing for both gene knockout and copy number increase, but detailed knowledge of the molecular outcomes of radiation treatment is lacking. To address this, we produced a genome re-sequenced panel of 1133 M2 generation rapeseed plants and analysed large-scale deletions, single nucleotide variants and small insertion–deletion variants affecting gene open reading frames. We show that high radiation doses (2000 Gy) are tolerated, gamma radiation and fast neutron radiation have similar impacts and that segments deleted from the genomes of some plants are inherited as additional copies by their siblings, enabling gene dosage decrease. Of relevance for species with larger genomes, we showed that these large-scale impacts can also be detected using transcriptome re-sequencing. To test the utility of the approach for predictive alteration of oil fatty acid composition, we produced lines with both decreased and increased copy numbers of Bna.FAE1 and confirmed the anticipated impacts on erucic acid content. We detected and tested a 21-base deletion expected to abolish function of Bna.FAD2.A5, for which we confirmed the predicted reduction in seed oil polyunsaturated fatty acid content. Our improved understanding of the molecular effects of radiation mutagenesis will underpin genomics-led approaches to more efficient introduction of novel genetic variation into the breeding of this crop and provides an exemplar for the predictive improvement of other crops.

KeywordsBrassica napus; Radiation mutagenesis; Vegetable oil
Year of Publication2023
JournalPlant Biotechnology Journal
Journal citationpp. 1-13
Digital Object Identifier (DOI)https://doi.org/10.1111/pbi.14220
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeGenomics-led improvement of biotic and abiotic stress tolerance in mustard rape for economic and environmental sustainability
Renewable Industrial Products from Rapeseed: RIPR
Green Engineering
Publisher's version
Output statusPublished
Publication dates
Online03 Nov 2024
Publication process dates
Accepted20 Oct 2023
PublisherWiley
ISSN1467-7644

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