Expression of castor LPAT2 enhances ricinoleic acid content at the sn-2 position of triacylglycerols in lesquerella seed

A - Papers appearing in refereed journals

Chen, G. Q., Van-Erp, H., Martin-Moreno, J., Johnson, K., Morales, E., Browse, J., Eastmond, P. J. and Lin, J-T. 2016. Expression of castor LPAT2 enhances ricinoleic acid content at the sn-2 position of triacylglycerols in lesquerella seed. International Journal Of Molecular Sciences. 17 (4), p. 507.

AuthorsChen, G. Q., Van-Erp, H., Martin-Moreno, J., Johnson, K., Morales, E., Browse, J., Eastmond, P. J. and Lin, J-T.
Abstract

Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%–17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%–14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production.

Keywordslesquerella; Physaria fendleri; lysophosphatidic acid acyltransferase; hydroxy fatty acid; seed triacylglycerol; metabolic engineering
Year of Publication2016
JournalInternational Journal Of Molecular Sciences
Journal citation17 (4), p. 507
Digital Object Identifier (DOI)doi:10.3390/ijms17040507
Open accessPublished as ‘gold’ (paid) open access
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
Publisher's version
Output statusPublished
Publication dates
Online06 Apr 2016
Publication process dates
Accepted30 Mar 2016
PublisherMDPI
MDPI
Copyright licenseCC BY
ISSN1422-0067

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