Expression of castor LPAT2 enhances ricinoleic acid content at the sn-2 position of triacylglycerols in lesquerella seed

A - Papers appearing in refereed journals

Chen, G. Q., Van-Erp, H., Martin-Moreno, J., Johnson, K., Morales, E., Browse, J., Eastmond, P. J. and Lin, J-T. 2016. Expression of castor LPAT2 enhances ricinoleic acid content at the sn-2 position of triacylglycerols in lesquerella seed. International Journal Of Molecular Sciences. 17 (4), p. 507.

AuthorsChen, G. Q., Van-Erp, H., Martin-Moreno, J., Johnson, K., Morales, E., Browse, J., Eastmond, P. J. and Lin, J-T.
Abstract

Lesquerella is a potential industrial oilseed crop that makes hydroxy fatty acid (HFA). Unlike castor its seeds are not poisonous but accumulate lesquerolic acid mostly at the sn-1 and sn-3 positions of triacylglycerol (TAG), whereas castor contains ricinoleic acid (18:1OH) at all three positions. To investigate whether lesquerella can be engineered to accumulate HFAs in the sn-2 position, multiple transgenic lines were made that express castor lysophosphatidic acid acyltransferase 2 (RcLPAT2) in the seed. RcLPAT2 increased 18:1OH at the sn-2 position of TAGs from 2% to 14%–17%, which resulted in an increase of tri-HFA-TAGs from 5% to 13%–14%. Our result is the first example of using a LPAT to increase ricinoleic acid at the sn-2 position of seed TAG. This work provides insights to the mechanism of HFA-containing TAG assembly in lesquerella and directs future research to optimize this plant for HFA production.

Keywordslesquerella; Physaria fendleri; lysophosphatidic acid acyltransferase; hydroxy fatty acid; seed triacylglycerol; metabolic engineering
Year of Publication2016
JournalInternational Journal Of Molecular Sciences
Journal citation17 (4), p. 507
Digital Object Identifier (DOI)doi:10.3390/ijms17040507
Open accessPublished as ‘gold’ (paid) open access
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
*Design Oilseeds (Peter Eastmond)
Publisher's version
Output statusPublished
Publication dates
Online06 Apr 2016
Publication process dates
Accepted30 Mar 2016
PublisherMDPI
MDPI
Copyright licenseCC BY
ISSN1422-0067

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