Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed

A - Papers appearing in refereed journals

Nogueira, M., Enfissi, E. M. A., Martínez Valenzuela, M. E., Menard, G. N., Driller, R. L., Eastmond, P. J., Schuch, W., Sandmann, G. and Fraser, P. D. 2017. Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. Proceedings of the National Academy of Sciences of the United States of America - PNAS. 114 (41), pp. 10876-10881.

AuthorsNogueira, M., Enfissi, E. M. A., Martínez Valenzuela, M. E., Menard, G. N., Driller, R. L., Eastmond, P. J., Schuch, W., Sandmann, G. and Fraser, P. D.
Abstract

Ketocarotenoids are high-value pigments used commercially across multiple industrial sectors as colorants and supplements. Chemical synthesis using petrochemical-derived precursors remains the production method of choice. Aquaculture is an example where ketocarotenoid supplementation of feed is necessary to achieve product viability. The biosynthesis of ketocarotenoids, such as canthaxanthin, phoenicoxanthin, or astaxanthin in plants is rare. In the present study, complex engineering of the carotenoid pathway has been performed to produce high-value ketocarotenoids in tomato fruit (3.0 mg/g dry weight). The strategy adopted involved pathway extension beyond β-carotene through the expression of the β-carotene hydroxylase (CrtZ) and oxyxgenase (CrtW) from Brevundimonas sp. in tomato fruit, followed by β-carotene enhancement through the introgression of a lycopene β-cyclase (β-Cyc) allele from a Solanum galapagense background. Detailed biochemical analysis, carried out using chromatographic, UV/VIS, and MS approaches, identified the predominant carotenoid as fatty acid (C14:0 and C16:0) esters of phoenicoxanthin, present in the S stereoisomer configuration. Under a field-like environment with low resource input, scalability was shown with the potential to deliver 23 kg of ketocarotenoid/hectare. To illustrate the potential of this “generally recognized as safe” material with minimal, low-energy bioprocessing, two independent aquaculture trials were performed. The plant-based feeds developed were more efficient than the synthetic feed to color trout flesh (up to twofold increase in the retention of the main ketocarotenoids in the fish fillets). This achievement has the potential to create a new paradigm in the renewable production of economically competitive feed additives for the aquaculture industry and beyond.

Keywordscarotenoids; genetic intervention; tomato; aquaculture; industrial biotechnology
Year of Publication2017
JournalProceedings of the National Academy of Sciences of the United States of America - PNAS
Journal citation114 (41), pp. 10876-10881
Digital Object Identifier (DOI)doi:10.1073/pnas.1708349114
Open accessPublished as ‘gold’ (paid) open access
FunderEuropean Union - EU
Biotechnology and Biological Sciences Research Council
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
DISCO
OPTICAR
KBBE III
CAROMAIZE
EUROCAROTEN
Project: 5263
*Design Oilseeds (Peter Eastmond)
TPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Publisher's version
Output statusPublished
Publication dates
Online25 Sep 2017
Publication process dates
Accepted01 Sep 2017
PublisherWashington, DC, National Academy Press
National Academy of Sciences of the United States of America
Copyright licenseCC BY
Grant IDBB/P001742/1
PIM2010PKB-00746
COST Action 15136
ISSN0027-8424

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