Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity

A - Papers appearing in refereed journals

Craddock, C. P., Adams, N., Kroon, J. T. M., Bryant, F. M., Hussey, P. J., Kurup, S. and Eastmond, P. J. 2017. Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity. The Plant Journal. 89 (1), pp. 3-14.

AuthorsCraddock, C. P., Adams, N., Kroon, J. T. M., Bryant, F. M., Hussey, P. J., Kurup, S. and Eastmond, P. J.
Abstract

Coordination of endomembrane biogenesis with cell cycle progression is considered to be important in maintaining cell function during growth and development. We previously showed that the disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in Arabidopsis thaliana stimulates biosynthesis of the major phospholipid phosphatidylcholine (PC) and causes expansion of the endoplasmic reticulum. Here we show that PC biosynthesis is repressed by disruption of the core cell cycle regulator CYCLIN‐DEPENDENT KINASE A;1 (CDKA;1) and that this repression is reliant on PAH. Furthermore, we show that cyclin‐dependent kinases (CDKs) phosphorylate PAH1 at serine 162, which reduces both its activity and membrane association. Expression of a CDK‐insensitive version of PAH1 with a serine 162 to alanine substitution represses PC biosynthesis and also reduces the rate of cell division in early leaf development. Together our findings reveal a physiologically important mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis to cell cycle progression in Arabidopsis.

KeywordsArabidopsis thaliana; phosphatidylcholine; phospholipid biosynthesis; membrane biogenesis; phosphatidic acid phosphohydrolase; phosphorylation; cell cycle; cyclin-dependent kinase
Year of Publication2017
JournalThe Plant Journal
Journal citation89 (1), pp. 3-14
Digital Object Identifier (DOI)doi:10.1111/tpj.13321
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
*Design Oilseeds (Peter Eastmond)
Designing oilseeds for nutrition and health
Publisher's version
Copyright license
CC BY
Output statusPublished
Publication dates
Online06 Sep 2016
Publication process dates
Accepted24 Aug 2016
Copyright licenseCC BY
PublisherWiley-Blackwell
ISSN0960-7412

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