Ethylene augments root hypoxia tolerance through amelioration of reactive oxygen species and growth cessation

A - Papers appearing in refereed journals

Liu, Z., Hartman, S., Van Veen, H., Zhang, H., Leeggangers, H. A. C. F., Martopawiro, S., Bosman, F., De Deugd, F., Su, P., Hummel, M., Rankenberg, T., Hassall, K. L., Bailey-Serres, J., Theodoulou, F. L., Voesenek, L. A. C. J. and Sasidharan, R. 2022. Ethylene augments root hypoxia tolerance through amelioration of reactive oxygen species and growth cessation. bioRxiv. https://doi.org/10.1101/2022.01.21.477196

AuthorsLiu, Z., Hartman, S., Van Veen, H., Zhang, H., Leeggangers, H. A. C. F., Martopawiro, S., Bosman, F., De Deugd, F., Su, P., Hummel, M., Rankenberg, T., Hassall, K. L., Bailey-Serres, J., Theodoulou, F. L., Voesenek, L. A. C. J. and Sasidharan, R.
Abstract

Flooded plants experience impaired gas diffusion underwater, leading to oxygen deprivation (hypoxia). The volatile plant hormone ethylene is rapidly trapped in submerged plant cells and is instrumental for enhanced hypoxia acclimation. However, the precise mechanisms underpinning ethylene-enhanced hypoxia survival remain unclear. We studied the effect of ethylene pre-treatment on hypoxia survival of primary Arabidopsis thaliana root tips. Both hypoxia itself and re-oxygenation following hypoxia are highly damaging to root tip cells and ethylene pre-treatments reduced this damage. Ethylene pre-treatment alone altered the available abundance of transcripts and proteins involved in hypoxia responses, root growth, translation and reactive oxygen species (ROS) homeostasis. Through imaging and manipulating ROS abundance in planta, we demonstrate that ethylene limits excessive ROS formation during hypoxia and subsequent re-oxygenation and improves oxidative stress survival. In addition, we show that ethylene leads to rapid root growth cessation and this quiescence behaviour contributes to enhanced hypoxia tolerance. Collectively, our results show that the early flooding signal ethylene modulates a variety of processes that all contribute to hypoxia survival.

Year of Publication2022
JournalbioRxiv
Digital Object Identifier (DOI)https://doi.org/10.1101/2022.01.21.477196
Open accessPublished as bronze (free) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeTPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
UK-China Joint Centre for Sustainable Intensification in Agriculture (CSIA).
Publisher's version
Output statusE-publication ahead of print
Publication dates
Online23 Jan 2022
PublisherPublic Library of Science (PLOS)

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