Pseudo half-molecules of the ABC transporter, COMATOSE, bind Pex19 and target to peroxisomes independently but are both required for activity

A - Papers appearing in refereed journals

Nyathi, Y., Zhang, X., Baldwin, J. M., Bernhardt, K., Johnson, B., Baldwin, S. A., Theodoulou, F. L. and Baker, A. 2012. Pseudo half-molecules of the ABC transporter, COMATOSE, bind Pex19 and target to peroxisomes independently but are both required for activity. FEBS Letters. 586 (16), pp. 2280-2286. https://doi.org/10.1016/j.febslet.2012.05.065

AuthorsNyathi, Y., Zhang, X., Baldwin, J. M., Bernhardt, K., Johnson, B., Baldwin, S. A., Theodoulou, F. L. and Baker, A.
Abstract

Peroxisomal ABC transporters of animals and fungi are "half-size'' proteins which dimerise to form a functional transporter. However, peroxisomal ABC transporters of land plants are synthesised as a single polypeptide which represents a fused heterodimer. The N- and C-terminal pseudo-halves of COMATOSE (CTS; AtABCD1) were expressed as separate polypeptides which bound Pex19 in vitro and targeted independently to the peroxisome membrane in yeast, where they were stable but not functional. When co-expressed, the pseudo-halves were fully functional as indicated by ATPase activity and rescue of the pxa1pxa2 Delta mutant for growth on oleate. The functional significance of heterodimer asymmetry is discussed.

KeywordsBiochemistry & Molecular Biology; Biophysics; Cell Biology
Year of Publication2012
JournalFEBS Letters
Journal citation586 (16), pp. 2280-2286
Digital Object Identifier (DOI)https://doi.org/10.1016/j.febslet.2012.05.065
PubMed ID22710125
Open accessPublished as green open access
FunderBoehringer Ingelheim Fonds
Biotechnology and Biological Sciences Research Council
Funder project or codeCentre for Crop Genetic Improvement (CGI)
Purification and functional characterisation of COMATOSE a peroxisomal ABC transporter from Arabidopsis thaliana
Lipid transport and metabolism
Publisher's version
PublisherWiley
Grant IDBB/F007108/1
BB/F007299/1
ISSN1873-3468

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