Cropping system exerts stronger influence on antibiotic resistance gene assemblages in greenhouse soils than reclaimed wastewater irrigation

A - Papers appearing in refereed journals

Liu, Y., Neal, A. L., Zhang, X., Fan, H., Liu, H. and Li, Z. 2022. Cropping system exerts stronger influence on antibiotic resistance gene assemblages in greenhouse soils than reclaimed wastewater irrigation . Journal Of Hazardous Materials. 425, p. 128046. https://doi.org/10.1016/j.jhazmat.2021.128046

AuthorsLiu, Y., Neal, A. L., Zhang, X., Fan, H., Liu, H. and Li, Z.
Abstract

The effects of reclaimed wastewater (RW) irrigation on the spread of antibiotic resistance genes (ARGs) in soil is modulated by a myriad of biotic and abiotic factors and their relative significance remains vague. We compared microbial communities, assemblages of genes associated with microbial resistance to antibiotics, biocides and metals, and insertion sequences (ISs) in soils following 16 years of crop irrigation with groundwater (GW), RW or alternately with GW and RW in two greenhouses with different cropping systems, using shotgun metagenome sequencing. The results showed that cropping system exerted greater influence than irrigation on the profile of ISs and resistance genes. This influence was most strongly associated with concentrations of copper, mercury and perfloxacin in the soils. There was no significant difference in soil ARG profiles between continuous RW irrigation and alternating GW and RW irrigation. Proteobacteria, Actinobacteria and Firmicutes and a limited number of ISs were closely associated with the detected ARGs. Most ARGs were found to co-occur with metal and biocide resistance genes through the mechanism of efflux pumps. These findings highlight the significance of understanding and improving crop management in mitigating the dissemination of ARGs in soils irrigated with RW.

KeywordsReclaimed wastewater; Cropping system; Metagenomic analyses; Irrigation; ARGs
Year of Publication2022
JournalJournal Of Hazardous Materials
Journal citation425, p. 128046
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jhazmat.2021.128046
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Output statusPublished
Publication dates
Online10 Dec 2021
Publication process dates
Accepted06 Dec 2021
PublisherElsevier Science Bv
ISSN0304-3894

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