A - Papers appearing in refereed journals
Van Kleef, P. J. M., Gao, J., Mol, S., Zwart, N., Zhang, H., Li, K. W. and De Boer, A. H. 2018. The Arabidopsis GORK K+-channel is phosphorylated by calcium-dependent protein kinase 21 (CPK21), which in turn is activated by 14-3-3 proteins. Plant Physiology and Biochemistry. 125, pp. 219-231. https://doi.org/10.1016/j.plaphy.2018.02.013
Authors | Van Kleef, P. J. M., Gao, J., Mol, S., Zwart, N., Zhang, H., Li, K. W. and De Boer, A. H. |
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Abstract | Potassium (K+) is a vital ion for many processes in the plant and fine-tuned ion channels control the K+-fluxes across the plasma membrane. GORK is an outward-rectifying K+-channel with important functions in stomatal closure and in root K+-homeostasis. In this study, post-translational modification of the Arabidopsis GORK ion channel and its regulation by 14-3-3 proteins was investigated. To investigate the possible interaction between GORK and 14-3-3s an in vivo pull-down from an Arabidopsis protein extract with recombinant GORK C-terminus (GORK-C) indeed identified endogenous 14-3-3s (LAMBDA, CHI, NU) as binding partners in a phosphorylation dependent manner. However, a direct interaction between 14-3-3's and GORK-C could not be demonstrated. Since the pull-down of 14-3-3s was phosphorylation dependent, we determined GORK-C as substrate for CPK21 phosphorylation and identified three CPK21 phospho-sites in the GORK protein (T344, S518 and S649). Moreover, interaction of 14-3-3 to CPK21 strongly stimulates its kinase activity; an effect that can result in increased GORK phosphorylation and change in activity. Using the non-invasive vibrating probe technique, we measured the predominantly GORK mediated salt induced K+-efflux from wild-type, gork, cpk21, aha2 and 14-3-3 mutant roots. The mutants cpk21 and aha2 did not show statistical significant differences compared to WT. However, two (out of six) 14-3-3 isoforms, CHI and PHI, have a clear function in the salt induced K+-efflux. In conclusion, our results show that GORK can be phosphorylated by CPK21 and suggest that 14-3-3 proteins control GORK activity through binding with and activation of CPK21. |
Keywords | 14-3-3 proteins; GORK; CPKs; Salt stress; Vibrating probe; Roots |
Year of Publication | 2018 |
Journal | Plant Physiology and Biochemistry |
Journal citation | 125, pp. 219-231 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.plaphy.2018.02.013 |
Open access | Published as non-open access |
Funder | Chinese Research Council |
Netherlands Organization for Scientific Research ( NWO) | |
Funder project or code | Substrates of the N-end rule of targeted protein degradation |
Tailoring Plant Metabolism (TPM) - Work package 1 (WP1) - High value lipids for health and industry | |
Designing oilseeds for nutrition and health | |
Output status | Published |
Publication dates | |
Online | 16 Feb 2018 |
Publication process dates | |
Accepted | 13 Feb 2018 |
Copyright license | CC BY |
ISSN | 0981-9428 |
Publisher | Elsevier |
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