Tailoring seed oil composition in the real world: optimising omega -3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa

A - Papers appearing in refereed journals

Usher, S. L., Han, L., Haslam, R. P., Michaelson, L. V., Sturtevant, D., Aziz, M., Chapman, K. D., Sayanova, O. V. and Napier, J. A. 2017. Tailoring seed oil composition in the real world: optimising omega -3 long chain polyunsaturated fatty acid accumulation in transgenic Camelina sativa. Scientific Reports. 7 (1), p. 6570.

AuthorsUsher, S. L., Han, L., Haslam, R. P., Michaelson, L. V., Sturtevant, D., Aziz, M., Chapman, K. D., Sayanova, O. V. and Napier, J. A.
Abstract

There is considerable interest in the de novo production of omega-3 long chain polyunsaturated fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), not least of all given the importance of these fatty acids in both aquaculture and human nutrition. Previously we have demonstrated the feasibility of using metabolic engineering in transgenic plants (Camelina sativa) to modify the seed oil composition to now include EPA and/or DHA. In this study, we further tailored the seed oil profile to reduce the omega-6 content, and evaluated the performance of such GM plants under field conditions (i.e. environmental releases), in terms of agronomic performance and also the lipidomic profile of seed oil. We used MALDI- mass spectrometry imaging to identify discrete tissue-types in the seed in which these non-native fatty acids preferentially accumulated. Collectively, these data provide new insights into the complexity of plant lipid metabolism and the challenges associated with predictive manipulation of these pathways. However, this study identified the likely dispensable nature of a Δ12-desturase activity in our omega-3 metabolic engineering rationales for Camelina.

Year of Publication2017
JournalScientific Reports
Journal citation7 (1), p. 6570
Digital Object Identifier (DOI)doi:10.1038/s41598-017-06838-0
PubMed ID28747792
PubMed Central IDPMC5529437
Open accessPublished as ‘gold’ (paid) open access
Funder project or codeCamelina
Designing oilseeds for nutrition and health
FunderBiotechnology and Biological Sciences Research Council
Publisher's versions41598-017-06838-0.pdf
Output statusPublished
Publication dates
Online12 Jun 2017
Publication process dates
Accepted07 Mar 2017
Copyright licenseCC BY
Collective titleRothamsted Research Press Release 26 July 2017 GM Plant promise fish oils aplenty 
PublisherNature Publishing Group
ISSN2045-2322

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