Accumulation and deposition of triacylglycerols in the starchy endosperm of wheat grain

A - Papers appearing in refereed journals

Gonzalez-Thuillier, I., Pellny, T. K., Tosi, P., Mitchell, R. A. C., Haslam, R. P. and Shewry, P. R. 2021. Accumulation and deposition of triacylglycerols in the starchy endosperm of wheat grain. Journal of Cereal Science. 98 (article), p. 103167. https://doi.org/10.1016/j.jcs.2021.103167

AuthorsGonzalez-Thuillier, I., Pellny, T. K., Tosi, P., Mitchell, R. A. C., Haslam, R. P. and Shewry, P. R.
Abstract

A combination of lipidomics, transcriptomics and bioimaging has been used to study triacylglycerol synthesis and deposition in the developing starchy endosperm of wheat. The content of TAG increased between 14 and 34 days after anthesis, from 50 to 115 mg/g dry wt and from about 35 to 175 mg/g dry wt in two experiments. The major fatty acids were C16 (palmitic C 16:0 and palmitoleic C16:1) and C18 (stearic C18:0, oleic C18:1, linoleic C18:2 and linolenic C18:3), with unsaturated fatty acids accounting for about 75-80% of the total throughout development. Linoleic acid (C18:2) was the major component at all stages and the proportion increased during development. Transcript profiling indicated that predominant route to TAG synthesis and oil accumulation is via the Kennedy pathway and diacylglycerol acyltransferase (DGAT) activity. Confocal microscopy of stained tissue sections showed that TAG accumulated in droplets concentrated in the cells below the sub-aleurone cells which are associated with protein. Transcripts encoding 16kd oleosins were also expressed, indicating that the oil droplets are stabilised by oleosin proteins.

KeywordsWheat; Starchy endosperm; White flour; Triacylglycerol; Oleosin
Year of Publication2021
JournalJournal of Cereal Science
Journal citation98 (article), p. 103167
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jcs.2021.103167
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online22 Jan 2021
Publication process dates
Accepted13 Jan 2021
PublisherAcademic Press Ltd- Elsevier Science Ltd
ISSN0733-5210

Permalink - https://repository.rothamsted.ac.uk/item/98305/accumulation-and-deposition-of-triacylglycerols-in-the-starchy-endosperm-of-wheat-grain

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