Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines

A - Papers appearing in refereed journals

Rakszegi, M., Darkó, E., Lovegrove, A., Molnár, M., Lang, L., Bedő, Z, Molnár-Láng, M. and Shewry, P. R. 2019. Drought stress affects the protein and dietary fiber content of wholemeal wheat flour in wheat/Aegilops addition lines. PLOS ONE. 14 (2), p. e0211892.

AuthorsRakszegi, M., Darkó, E., Lovegrove, A., Molnár, M., Lang, L., Bedő, Z, Molnár-Láng, M. and Shewry, P. R.
Abstract

Wild relatives of wheat, such as Aegilops spp. are potential sources of genes conferring tolerance to drought stress. As drought stress affects seed composition, the main goal of the present study was to determine the effects of drought stress on the content and composition of the grain storage protein (gliadin (Gli), glutenin (Glu), unextractable polymeric proteins (UPP%) and dietary fiber (arabinoxylan, β-glucan) components of hexaploid bread wheat (T. aestivum) lines containing added chromosomes from Ae. biuncialis or Ae. geniculata. Both Aegilops parents have higher contents of protein and β-glucan and higher proportions of water-soluble arabinoxylans (determined as pentosans) than wheat when grown under both well-watered and drought stress conditions. In general, drought stress resulted in increased contents of protein and total pentosans in the addition lines, while the β-glucan content decreased in many of the addition lines. The differences found between the wheat/Aegilops addition lines and wheat parents under well-watered conditions were also manifested under drought stress conditions: Namely, elevated β-glucan content was found in addition lines containing chromosomes 5Ug, 7Ug and 7Mb, while chromosomes 1Ub and 1Mg affected the proportion of polymeric proteins (determined as Glu/Gli and UPP%, respectively) under both well-watered and drought stress conditions. Furthermore, the addition of chromosome 6Mg decreased the WE-pentosan content under both conditions. The grain composition of the Aegilops accessions was more stable under drought stress than that of wheat, and wheat lines with the added Aegilops chromosomes 2Mg and 5Mg also had more stable grain protein and pentosan contents. The negative effects of drought stress on both the physical and compositional properties of wheat were also reduced by the addition of these. These results suggest that the stability of the grain composition could be improved under drought stress conditions by the intraspecific hybridization of wheat with its wild relatives.

Year of Publication2019
JournalPLOS ONE
Journal citation14 (2), p. e0211892
Digital Object Identifier (DOI)doi:10.1371/journal.pone.0211892
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
Publisher's version
Output statusPublished
Publication dates
Online05 Feb 2019
Publication process dates
Accepted09 Oct 2018
Copyright licenseCC BY
PublisherPublic Library of Science (PLOS)
ISSN1932-6203

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