The location of iron and zinc in grain of conventional and biofortified lines of sorghum

A - Papers appearing in refereed journals

Gaddameedi, A., Sheraz, S., Kumar, A., Li, K., Pellny, T. K., Gupta, R., Wan, Y., Moore, K. L. and Shewry, P. R. 2022. The location of iron and zinc in grain of conventional and biofortified lines of sorghum. Journal of Cereal Science. 107, p. 103531. https://doi.org/10.1016/j.jcs.2022.103531

AuthorsGaddameedi, A., Sheraz, S., Kumar, A., Li, K., Pellny, T. K., Gupta, R., Wan, Y., Moore, K. L. and Shewry, P. R.
Abstract

Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that “hot spots” of 56Fe+ and 64Zn+ were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained 31P16O+ indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of 56Fe+ and 64Zn+ were also observed in the protein matrix of these cells.

KeywordsSorghum grain; Biofortification; NanoSIMS; Localisation; Iron; Zinc
Year of Publication2022
JournalJournal of Cereal Science
Journal citation107, p. 103531
Digital Object Identifier (DOI)https://doi.org/10.1016/j.jcs.2022.103531
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience
CROPNUT: increasing iron in cereals
Indo-UK Centre for the improvement of Nitrogen use Efficiency in Wheat (INEW)
Publisher's version
Output statusPublished
Publication dates
Online06 Aug 2022
Publication process dates
Accepted19 Jul 2022
PublisherAcademic Press Ltd- Elsevier Science Ltd
ISSN0733-5210

Permalink - https://repository.rothamsted.ac.uk/item/989q6/the-location-of-iron-and-zinc-in-grain-of-conventional-and-biofortified-lines-of-sorghum

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