A - Papers appearing in refereed journals
Gaddameedi, A., Sheraz, S., Kumar, A., Li, K., Pellny, T. K., Gupta, R., Wan, Y., Moore, K. L. and Shewry, P. R. 2022. The location of iron and zinc in grain of conventional and biofortified lines of sorghum. Journal of Cereal Science. 107, p. 103531. https://doi.org/10.1016/j.jcs.2022.103531
Authors | Gaddameedi, A., Sheraz, S., Kumar, A., Li, K., Pellny, T. K., Gupta, R., Wan, Y., Moore, K. L. and Shewry, P. R. |
---|---|
Abstract | Sorghum is an important source of dietary iron (Fe) and zinc (Zn) in parts of Africa and India, but there is a need to increase their concentrations to meet dietary requirements. Grains of a genetically biofortified sorghum line (Parbhani Shakti) had higher concentrations of Fe and Zn than a control line (M35-1). Analysis at the tissue level by histochemical staining and at the cellular level using NanoSIMS showed that both minerals are concentrated in the aleurone layer and in the scutellum of the embryo, with Zn also being concentrated in the embryonic axis. However, NanoSIMS showed that “hot spots” of 56Fe+ and 64Zn+ were also present in the sub-aleurone and starchy endosperm cells. Most of these hot spots also contained 31P16O+ indicating that the Fe and Zn are present as phytates, as in the aleurone and scutellum cells. Low concentrations of 56Fe+ and 64Zn+ were also observed in the protein matrix of these cells. |
Keywords | Sorghum grain; Biofortification; NanoSIMS; Localisation; Iron; Zinc |
Year of Publication | 2022 |
Journal | Journal of Cereal Science |
Journal citation | 107, p. 103531 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.jcs.2022.103531 |
Open access | Published as ‘gold’ (paid) open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | DFW - Designing Future Wheat - Work package 2 (WP2) - Added value and resilience |
CROPNUT: increasing iron in cereals | |
Indo-UK Centre for the improvement of Nitrogen use Efficiency in Wheat (INEW) | |
Publisher's version | |
Output status | Published |
Publication dates | |
Online | 06 Aug 2022 |
Publication process dates | |
Accepted | 19 Jul 2022 |
Publisher | Academic Press Ltd- Elsevier Science Ltd |
ISSN | 0733-5210 |
Permalink - https://repository.rothamsted.ac.uk/item/989q6/the-location-of-iron-and-zinc-in-grain-of-conventional-and-biofortified-lines-of-sorghum