A - Papers appearing in refereed journals
Chatzifragkou, A., Prabhakumari, P. C., Kosik, O., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D. 2016. Extractability and characteristics of proteins deriving from wheat DDGS. Food Chemistry. 198 (1 May), pp. 12-19. https://doi.org/10.1016/j.foodchem.2015.11.036
Authors | Chatzifragkou, A., Prabhakumari, P. C., Kosik, O., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D. |
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Abstract | Wheat Distillers’ Dried Grains with Solubles (DDGS) and in-process samples were used for protein extraction. Prolamins were the predominant protein components in the samples. The absence of extractable α- and γ-gliadins in DDGS indicated protein aggregation during the drum drying processing stage. Prolamin extraction was performed using 70% (v/v) ethanol or alkaline–ethanol solution in the presence of reducing agent. DDGS extracts had relatively low protein contents (14–44.9%, w/w), regardless of the condition applied. The wet solids were the most suitable raw material for protein extraction, with recovery yields of ∼55% (w/w) and protein content of ∼58% (w/w) in 70% (v/v) ethanol. Protein extracts from wet solids were significantly rich in glutamic acid and proline. Mass balance calculations demonstrated the high carbohydrate content (∼50%, w/w) of solid residues. Overall, the feasibility of utilising in-process samples of DDGS for protein extraction with commercial potential was demonstrated. |
Keywords | DDGS; Protein; Wheat; Distillery; Extraction; In-process samples; Amino acids |
Year of Publication | 2016 |
Journal | Food Chemistry |
Journal citation | 198 (1 May), pp. 12-19 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.foodchem.2015.11.036 |
Open access | Published as green open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | Designing Seeds for Nutrition and Health (DS) |
Development of a process scheme for the production of high value functional products from DDGS | |
Design Cereal Seeds [Shewry/Mitchell] | |
Publisher's version | Copyright license Publisher copyright |
Accepted author manuscript | Copyright license CC BY-NC-ND |
Output status | Published |
Publication dates | |
Online | 11 Nov 2015 |
Publication process dates | |
Accepted | 06 Nov 2015 |
ISSN | 0308-8146 |
Publisher | Elsevier Sci Ltd |
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