Extractability and characteristics of proteins deriving from wheat DDGS

A - Papers appearing in refereed journals

Chatzifragkou, A., Prabhakumari, P. C., Kosik, O., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D. 2016. Extractability and characteristics of proteins deriving from wheat DDGS. Food Chemistry. 198 (1 May), pp. 12-19.

AuthorsChatzifragkou, A., Prabhakumari, P. C., Kosik, O., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D.
Abstract

Wheat Distillers’ Dried Grains with Solubles (DDGS) and in-process samples were used for protein extraction. Prolamins were the predominant protein components in the samples. The absence of extractable α- and γ-gliadins in DDGS indicated protein aggregation during the drum drying processing stage. Prolamin extraction was performed using 70% (v/v) ethanol or alkaline–ethanol solution in the presence of reducing agent. DDGS extracts had relatively low protein contents (14–44.9%, w/w), regardless of the condition applied. The wet solids were the most suitable raw material for protein extraction, with recovery yields of ∼55% (w/w) and protein content of ∼58% (w/w) in 70% (v/v) ethanol. Protein extracts from wet solids were significantly rich in glutamic acid and proline. Mass balance calculations demonstrated the high carbohydrate content (∼50%, w/w) of solid residues. Overall, the feasibility of utilising in-process samples of DDGS for protein extraction with commercial potential was demonstrated.

KeywordsDDGS; Protein; Wheat; Distillery; Extraction; In-process samples; Amino acids
Year of Publication2016
JournalFood Chemistry
Journal citation198 (1 May), pp. 12-19
Digital Object Identifier (DOI)doi:10.1016/j.foodchem.2015.11.036
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Seeds for Nutrition and Health (DS)
Development of a process scheme for the production of high value functional products from DDGS
Design Cereal Seeds [Shewry/Mitchell]
Publisher's version
Copyright license
Publisher copyright
Accepted author manuscript
Copyright license
CC BY-NC-ND
Output statusPublished
Publication dates
Online11 Nov 2015
Publication process dates
Accepted06 Nov 2015
ISSN0308-8146
PublisherElsevier Sci Ltd

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