Carbohydrate structural analysis of wheat flour arabinogalactan protein

A - Papers appearing in refereed journals

Tryfona, T., Liang, H-C., Kotake, T., Kaneko, S., Marsh, J., Ichinose, H., Lovegrove, A., Tsumuraya, Y., Shewry, P. R., Stephens, E. and Dupree, P. 2010. Carbohydrate structural analysis of wheat flour arabinogalactan protein. Carbohydrate Research. 345 (18), pp. 2648-2656.

AuthorsTryfona, T., Liang, H-C., Kotake, T., Kaneko, S., Marsh, J., Ichinose, H., Lovegrove, A., Tsumuraya, Y., Shewry, P. R., Stephens, E. and Dupree, P.
Abstract

The water-extractable arabinogalactan protein (AGP) was isolated from bread wheat flour (Triticum aestivum L. variety Cadenza) and the structure of the arabinogalactan (AG) carbohydrate component was studied. Oligosaccharides, released by hydrolysis of the AG with a range of AGP-specific enzymes, were characterised by Matrix Assisted Laser Desorption Ionisation (MALDI)-Time of Flight (ToF)-Mass Spectrometry (MS), MALDI-ToF/ToF high energy collision induced dissociation (CID) and Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE). The AG is composed of a β-(1→3)-d-galactan backbone with β-(1→6)-d-galactan side chains. These side chains are highly variable in length, from one to at least 20 Gal residues and are highly substituted with α-l-Araf. Single GlcA residues are also present at the non-reducing termini of some short β-(1→6)-galactan side chains. In addition, the β-(1→6)-galactan side chains are also substituted with β-l-Arap. We propose a polysaccharide structure of the wheat flour AGP that is substantially revised from earlier models.

Year of Publication2010
JournalCarbohydrate Research
Journal citation345 (18), pp. 2648-2656
Digital Object Identifier (DOI)doi:10.1016/j.carres.2010.09.018
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Crop Genetic Improvement (CGI)
Cereal seed composition and end use quality
Publisher's version
Copyright license
Publisher copyright
File Access Level
Restricted
Output statusPublished
Publication dates
Online21 Sep 2010
Publication process dates
Accepted16 Sep 2010
PublisherElsevier Sci Ltd
ISSN0008-6215

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