A novel bioinformatics approach identifies candidate genes for the synthesis and feruloylation of arabinoxylan

A - Papers appearing in refereed journals

Mitchell, R. A. C., Dupree, P. and Shewry, P. R. 2007. A novel bioinformatics approach identifies candidate genes for the synthesis and feruloylation of arabinoxylan. Plant Physiology. 144 (May), pp. 43-53. https://doi.org/10.1104/pp.106.094995

AuthorsMitchell, R. A. C., Dupree, P. and Shewry, P. R.

Arabinoxylans (AXs) are major components of graminaceous plant cell walls, including those in the grain and straw of economically important cereals. Despite some recent advances in identifying the genes encoding biosynthetic enzymes for a number of other plant cell wall polysaccharides, the genes encoding enzymes of the final stages of AX synthesis have not been identified. We have therefore adopted a novel bioinformatics approach based on estimation of differential expression of orthologous genes between taxonomic divisions of species. Over 3 million public domain cereal and dicot expressed sequence tags were mapped onto the complete sets of rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana) genes, respectively. It was assumed that genes in cereals involved in AX biosynthesis would be expressed at high levels and that their orthologs in dicotyledonous plants would be expressed at much lower levels. Considering all rice genes encoding putative glycosyl transferases (GTs) predicted to be integral membrane proteins, genes in the GT43, GT47, and GT61 families emerged as much the strongest candidates. When the search was widened to all other rice or Arabidopsis genes predicted to encode integral membrane proteins, cereal genes in Pfam family PF02458 emerged as candidates for the feruloylation of AX. Our analysis, known activities, and recent findings elsewhere are most consistent with genes in the GT43 families encoding b-1,4-xylan synthases, genes in the GT47 family encoding xylan a-1,2- or a-1,3-arabinosyl transferases, and genes in the GT61 family encoding feruloyl-AX b-1,2-xylosyl transferases. 

KeywordsRRES175; 175_Plant sciences; 175_Bioinformatics
Year of Publication2007
JournalPlant Physiology
Journal citation144 (May), pp. 43-53
Digital Object Identifier (DOI)https://doi.org/10.1104/pp.106.094995
Open accessPublished as bronze (free) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeCentre for Crop Genetic Improvement (CGI)
HEALTHGRAIN: Exploring bioactivity of European cereals for improved nutrition and health benefits
Structural and protein engineering studies of novel cereal and oilseed proteins with functional properties or biological activity
Publisher's version
Output statusPublished
Publication dates
Online07 Mar 2006
Publication process dates
Accepted06 Mar 2007
PublisherAmerican Society of Plant Biologists, (ASPB), Rockville, MD
American Society of Plant Biologists (ASPB)

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