Recruitment of glutathione into the nucleus during cell proliferation adjusts whole-cell redox homeostasis in Arabidopsis thaliana and lowers the oxidative defence shield

A - Papers appearing in refereed journals

Vivancos, P. D., Dong, Y., Ziegler, K., Markovic, J., Pallardo, F. V., Pellny, T. K., Verrier, P. J. and Foyer, C. H. 2010. Recruitment of glutathione into the nucleus during cell proliferation adjusts whole-cell redox homeostasis in Arabidopsis thaliana and lowers the oxidative defence shield. The Plant Journal. 64 (5), pp. 825-838.

AuthorsVivancos, P. D., Dong, Y., Ziegler, K., Markovic, J., Pallardo, F. V., Pellny, T. K., Verrier, P. J. and Foyer, C. H.
Abstract

P>Cellular redox homeostasis and signalling are important in progression of the eukaryotic cell cycle. In animals, the low-molecular-weight thiol tripeptide glutathione (GSH) is recruited into the nucleus early in the cell proliferation cycle. To determine whether a similar process occurs in plants, we studied cell proliferation in Arabidopsis thaliana. We show that GSH co-localizes with nuclear DNA during the proliferation of A. thaliana cells in culture. Moreover, GSH localization in the nucleus was observed in dividing pericycle cells of the lateral root meristem. There was pronounced accumulation of GSH in the nucleus at points in the growth cycle at which a high percentage of the cells were in G(1) phase, as identified by flow cytometry and marker transcripts. Recruitment of GSH into the nucleus led to a high abundance of GSH in the nucleus (GSHn) and severe depletion of the cytoplasmic GSH pool (GSHc). Sequestration of GSH in the nucleus was accompanied by significant decreases in transcripts associated with oxidative signalling and stress tolerance, and an increase in the abundance of hydrogen peroxide, an effect that was enhanced when the dividing cells were treated with salicylic acid. Total cellular GSH and the abundance of GSH1 and GSH2 transcripts increased after the initial recruitment of GSH into the nucleus. We conclude that GSH recruitment into the nucleus during cell proliferation has a profound effect on the whole-cell redox state. High GSHn levels trigger redox adjustments in the cytoplasm, favouring decreased oxidative signalling and enhanced GSH synthesis.

KeywordsPlant Sciences
Year of Publication2010
JournalThe Plant Journal
Journal citation64 (5), pp. 825-838
Digital Object Identifier (DOI)doi:10.1111/j.1365-313X.2010.04371.x
PubMed ID21105929
Open accessPublished as non-open access
FunderFundacion Seneca (Comunidad Autonoma de la Region de Murcia)
Biotechnology and Biological Sciences Research Council
Ministry of Science and Innovation (Spain)
Funder project or codeCentre for Crop Genetic Improvement (CGI)
Centre for Mathematical and Computational Biology (MCB)
Plant growth responses to the environment: interfaces between anti-oxidants, PARP and the cell cycle [Hedden]
Project: 9013
ISSN09607412
0960-7412
PublisherWiley
Grant IDBB/C51508X/1
SAF2008-01338

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