Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial.

A - Papers appearing in refereed journals

Raffan, S., Oddy, J., Mead, A., Barker, G., Curtis, T. Y., Usher, S. L., Burt, C. and Halford, N. G. 2023. Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial. Plant Biotechnology Journal. 21 (6), pp. 1097-1099. https://doi.org/10.1111/pbi.14026

AuthorsRaffan, S., Oddy, J., Mead, A., Barker, G., Curtis, T. Y., Usher, S. L., Burt, C. and Halford, N. G.
Abstract

We reported in this journal in 2021 the generation of wheat genotypes in which the asparagine synthetase gene, TaASN2, had been ‘knocked out’ using CRISPR-Cas9 (Raffan et al. 2021). The editing had been achieved by introducing genes encoding the Cas9 nuclease, four guide RNAs (gRNAs) and a Bar marker gene into wheat (Triticum aestivum) cv. Cadenza. Here we report the results of a field trial of Line 178.35, an A genome null for TaASN2, and total nulls, 23.60 and 23.75 (Raffan et al., 2021). Also included were four AB genome nulls, referred to as TILLING lines 1-4, derived from a selected line of a mutant population produced by ethyl methanesulphonate treatment of wheat cv. Cadenza seeds (Rakszegi et al., 2010). The mutated TaASN2-A2 gene from this line was backcrossed into the cv. Claire background to generate AB genome nulls (cv. Claire lacks a B genome TaASN2 gene due to a ‘natural’ deletion (Oddy et al., 2021)).

KeywordsWheat; CRISPR-Cas9; Field trial; Acrylamide; Asparagine synthetase
Year of Publication2023
JournalPlant Biotechnology Journal
Journal citation21 (6), pp. 1097-1099
Digital Object Identifier (DOI)https://doi.org/10.1111/pbi.14026
Web address (URL)https://onlinelibrary.wiley.com/doi/10.1111/pbi.14026
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeField assessment of ultra-low asparagine, low acrylamide, gene edited wheat
Crop management strategies for low asparagine grains to limit acrylamide-forming potential
Designing Future Wheat (DFW) [ISPG]
Publisher's version
Supplemental file
Output statusPublished
Publication dates
Online09 Feb 2023
Publication process dates
Accepted06 Feb 2023
PublisherWiley
ISSN1467-7644

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