Insights into Pasteurellaceae carriage dynamics in the nasal passages of healthy beef calves

A - Papers appearing in refereed journals

Lee, M. R. F., Bailey, M., Mead, A., Morales-Aza, B., Reynolds, R., Vipond, B., Finn, A., Eisler, M.C. and Thomas, A.C. 2019. Insights into Pasteurellaceae carriage dynamics in the nasal passages of healthy beef calves. Scientific Reports. 9 (11943), p. 11943.

AuthorsLee, M. R. F., Bailey, M., Mead, A., Morales-Aza, B., Reynolds, R., Vipond, B., Finn, A., Eisler, M.C. and Thomas, A.C.
Abstract

We investigated three bovine respiratory pathobionts in healthy cattle using qPCR optimised and validated to quantify Histophilus somni, Mannheimia haemolytica and Pasteurella multocida over a wide dynamic range. A longitudinal study was conducted to investigate the carriage and density of these bacteria in the nasal passages of healthy beef calves (n=60) housed over winter in an experimental farm setting. The three pathobiont species exhibited remarkably different carriage rates and density profiles. At housing, high carriage rates were observed for P. multocida (95%), and H. somni (75%), while fewer calves were positive for M. haemolytica (13%). Carriage rates for all three bacterial species declined over the 75-day study, but not all individuals became colonised despite sharing of environment and airspace. Colonisation patterns ranged from continuous to intermittent and were different among pathobiont species. Interval-censored exponential survival models estimated the median duration of H. somni and P. multocida carriage at 14.8 (CI95%: 10.6–20.9) and 55.5 (CI95%: 43.3–71.3) days respectively, and found higher density P. multocida carriage was associated with slower clearance (p = 0.036). This work offers insights into the dynamics of pathobiont carriage and provides a potential platform for further data collection and modelling studies.

KeywordsBacteriology; Infectious-disease epidemiology
Year of Publication2019
JournalScientific Reports
Journal citation9 (11943), p. 11943
Digital Object Identifier (DOI)doi:10.1038/s41598-019-48007-5
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeThe North Wyke Farm Platform- National Capability [2017-22]
Publisher's version
Output statusPublished
Publication dates
Online16 Aug 2019
Publication process dates
Accepted16 Jul 2019
Copyright licenseCC BY
PublisherNature Publishing Group
ISSN2045-2322

Permalink - https://repository.rothamsted.ac.uk/item/8wyx2/insights-into-pasteurellaceae-carriage-dynamics-in-the-nasal-passages-of-healthy-beef-calves

11 total views
9 total downloads
4 views this month
3 downloads this month