In vitro fermentation patterns and methane output of perennial ryegrass differing in water-soluble carbohydrate and nitrogen concentrations

A - Papers appearing in refereed journals

Rivero, M. J., Keim, J. P., Balocchi, O. A. and Lee, M. R. F. 2020. In vitro fermentation patterns and methane output of perennial ryegrass differing in water-soluble carbohydrate and nitrogen concentrations. Animals. 10 (1076), p. 1076. https://doi.org/10.3390/ani10061076

AuthorsRivero, M. J., Keim, J. P., Balocchi, O. A. and Lee, M. R. F.
Abstract

The objective of this study was to determine the effect of perennial ryegrass forages differing in their concentration of water-soluble carbohydrates (WSC) and crude protein (CP), and collected in spring and autumn, on in vitro rumen fermentation variables, nitrogen (N) metabolism indicators and methane (CH4) output using a batch culture system. Two contrasting perennial ryegrass pastures, sampled both in autumn and spring, were used: high (HS) and low (LS) sugar pastures with WSC concentrations of 322 and 343 g/kg for HS (autumn and spring) and, 224 and 293 g/kg for LS in autumn and spring, respectively. Duplicates were incubated for 24 h with rumen inocula in three different days (blocks). Headspace gas pressure was measured at: 2, 3, 4, 5, 6, 8, 10, 12, 18 and 24 h and CH4 concentration was determined. The residuals were analysed for individual volatile fatty acids (VFA) concentrations, total N, ammonia-N (NH3-N) and neutral detergent insoluble N. Another set of duplicates was incubated for 4 h for VFA and NH3-N determination. The HS produced more gas (218 vs 204 mL/g OM), tended to increase total VFA production (52.0 mM vs 49.5 mM at 24 h), reduced acetate:propionate ratio (2.52 vs 3.20 at 4 h and 2.85 vs 3.19 at 24 h) and CH4 production relative to total gas production (15.6 vs 16.8 mL/100 mL), and improved N use efficiency (22.1 vs 20.9). The contrasting chemical composition modified in vitro rumen fermentation tending to increase total VFA production, reduce acetate:propionate ratio and CH4 concentration, and improve N use efficiency through lower rumen NH3-N.

KeywordsHigh sugar grass; Greenhouse gases; Nutrient use efficiency; Volatile fatty acids; Fertilisation rate; Defoliation frequency
Year of Publication2020
JournalAnimals
Journal citation10 (1076), p. 1076
Digital Object Identifier (DOI)https://doi.org/10.3390/ani10061076
Open accessPublished as ‘gold’ (paid) open access
FunderBBSRC Industrial Strategy Challenge
Funder project or codeS2N - Soil to Nutrition - Work package 2 (WP2) - Adaptive management systems for improved efficiency and nutritional quality
Publisher's version
Output statusPublished
Publication dates
Online22 Jun 2020
Publication process dates
Accepted17 Jun 2020
PublisherMDPI
ISSN2076-2615

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