An altered tocopherol composition in chloroplasts reduces plant resistance to Botrytis cinerea

A - Papers appearing in refereed journals

Cela, J., Tweed, J. K. S., Sivakumaran, A., Lee, M. R. F., Mur, L. A. J. and Munne-Bosch, S. 2018. An altered tocopherol composition in chloroplasts reduces plant resistance to Botrytis cinerea. Plant Physiology and Biochemistry. 127, pp. 200-210.

AuthorsCela, J., Tweed, J. K. S., Sivakumaran, A., Lee, M. R. F., Mur, L. A. J. and Munne-Bosch, S.
Abstract

Tocopherols are lipid-soluble antioxidants that contribute to plant resistance to abiotic stresses. However, it is still unknown to what extent alterations in tocopherol composition can affect the plant response to biotic stresses. The response to bacterial and fungal attack of the vte1 mutant of Arabidopsis thaliana, which lacks both α- and γ-tocopherol, was compared to that of the vte4 mutant (which lacks α- but accumulates γ-tocopherol) and the wild type (with accumulates α-tocopherol in leaves). Both mutants exhibited similar kinetics of cell death and resistance in response to Pseudomonas syringae. In contrast, both mutants exhibited delayed resistance when infected with Botrytis cinerea. Lipid and hormonal profiling was employed with the aim of assessing the underlying cause of this differential phenotype. Although an altered tocopherol composition in both mutants strongly influenced fatty acid composition, and strongly altered jasmonic acid and cytokinin contents upon infection with B. cinerea, differences between genotypes in these phytohormones were observed during late stages of infection only. By contrast, genotype-related effects on lipid peroxidation, as indicated by malondialdehyde accumulation, were observed early upon infection with B. cinerea. We conclude that an altered tocopherol composition in chloroplasts may negatively influence the plant response to biotic stress in Arabidopsis thaliana through changes in the membrane fatty acid composition, enhanced lipid peroxidation and delayed defence activation when challenged with B. cinerea.

KeywordsBiotic stress; Hormonal balance; Jasmonic acid; Lipid profile; Tocopherols
Year of Publication2018
JournalPlant Physiology and Biochemistry
Journal citation127, pp. 200-210
Digital Object Identifier (DOI)doi:10.1016/j.plaphy.2018.03.033
Open accessPublished as non-open access
Funder project or codeIDG Bristol University / North Wyke [2012-2017]
Output statusPublished
Publication dates
Online28 Mar 2018
Publication process dates
Accepted27 Mar 2018
PublisherElsevier France-Editions Scientifiques Medicales Elsevier
Copyright licenseCC BY
ISSN0981-9428

Permalink - https://repository.rothamsted.ac.uk/item/84769/an-altered-tocopherol-composition-in-chloroplasts-reduces-plant-resistance-to-botrytis-cinerea

Restricted files

Publisher's version

Under embargo indefinitely

22 total views
2 total downloads
0 views this month
0 downloads this month