Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis

A - Papers appearing in refereed journals

Liu, D., Huang, X., Jing, W., An, X., Zhang, Q., Zhang, H., Zhou, J-J., Zhang, Y. and Guo, Y. 2018. Identification and functional analysis of two P450 enzymes of Gossypium hirsutum involved in DMNT and TMTT biosynthesis. Plant Biotechnology Journal. 16 (2), pp. 581-590.

AuthorsLiu, D., Huang, X., Jing, W., An, X., Zhang, Q., Zhang, H., Zhou, J-J., Zhang, Y. and Guo, Y.
Abstract

Summary The homoterpenes (3E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT) are major herbivore-induced plant volatiles that can attract predatory or parasitic arthropods to protect injured plants from herbivore attack. In this study, DMNT and TMTT were confirmed to be emitted from cotton (Gossypium hirsutum) plants infested with chewing caterpillars or sucking bugs. Two CYP genes (GhCYP82L1 and GhCYP82L2) involved in homoterpene biosynthesis in G. hirsutum were newly identified and characterized. Yeast recombinant expression and enzyme assays indicated that the two GhCYP82Ls are both responsible for the conversion of (E)-nerolidol to DMNT and (E,E)-geranyllinalool to TMTT. The two heterologously expressed proteins without cytochrome P450 reductase fail to convert the substrates to homoterpenes. Quantitative real-time PCR (qPCR) analysis suggested that the two GhCYP82L genes were significantly up-regulated in leaves and stems of G. hirsutum after herbivore attack. Subsequently, electroantennogram recordings showed that electroantennal responses of Microplitis mediator and Peristenus spretus to DMNT and TMTT were both dose dependent. Laboratory behavioural bioassays showed that females of both wasp species responded positively to DMNT and males and females of M. mediator could be attracted by TMTT. The results provide a better understanding of homoterpene biosynthesis in G. hirsutum and of the potential influence of homoterpenes on the behaviour of natural enemies, which lay a foundation to study genetically modified homoterpene biosynthesis and its possible application in agricultural pest control.

Year of Publication2018
JournalPlant Biotechnology Journal
Journal citation16 (2), pp. 581-590
Digital Object Identifier (DOI)doi:10.1111/pbi.12797
Open accessPublished as ‘gold’ (paid) open access
FunderNational Basic Research Program of China
National Natural Science Foundation of China
Publisher's version
Output statusPublished
Publication dates
Online15 Jul 2017
Publication process dates
Accepted11 Jul 2017
PublisherWiley
Copyright licenseCC BY
Grant ID2012CB114104
31272048
31471778
31672038
31621064
ISSN1467-7644

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