Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, Aphis gossypii

A - Papers appearing in refereed journals

Li, F., Venthur, H., Wang, S., Homem, R. A. and Zhou, J-J. 2021. Evidence for the Involvement of the Chemosensory Protein AgosCSP5 in Resistance to Insecticides in the Cotton Aphid, Aphis gossypii. Insects. 12 (4), p. 335. https://doi.org/10.3390/insects12040335

AuthorsLi, F., Venthur, H., Wang, S., Homem, R. A. and Zhou, J-J.
Abstract

It has been speculated that insect chemosensory proteins (CSPs) may have additional roles beyond olfaction. In this study, the phylogenetic and genomic analyses of the CSPs of the cotton aphid, Aphis gossypii, revealed the presence of gene gain-and-loss among different aphid field populations. Differential expressions of eight CSP genes were demonstrated after treatments with insecticides of different modes of action. The expression of AgosCSP5 was significantly upregulated by the insecticide treatments in a dose-dependent manner. The Drosophila flies overexpressing AgosCSP5 were significantly less susceptible to the insecticides, omethoate, imidacloprid and cypermethrin but not to deltamethrin and tau-fluvalinate, compared with control flies. The transgenic Drosophila flies exhibited an LC50 resistance ratio of 2.6 to omethoate, compared with control flies. Likewise, the mortality of the transgenic flies to imidacloprid and cypermethrin was significantly lower than that of the control flies (p < 0.01). Homology modelling, molecular docking and dynamic simulation supported the interactions and revealed a higher stability of AgosCSP5/insecticide complexes than AgosCSP5/semiochemical complexes. Our study demonstrates for first time the in vivo evidence for the involvement of CSP genes in insecticide resistance of crop insect pests and provides new insights of the newly discovered CSP-mediated insect resistance mechanism to insecticides.

KeywordsChemosensory protein; Pesticide resistance; Insect pest management
Year of Publication2021
JournalInsects
Journal citation12 (4), p. 335
Digital Object Identifier (DOI)https://doi.org/10.3390/insects12040335
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Publisher's version
Output statusPublished
Publication dates
Online09 Apr 2021
Publication process dates
Accepted04 Apr 2021
ISSN2075-4450
PublisherMDPI

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