A - Papers appearing in refereed journals
Troczka, B. J., Homem, R. A., Reid, R., Beadle, K., Kohler, M, Zaworra, M, Field, L. M., Williamson, M. S., Nauen, R., Bass, C. and Davies, T. G. E. 2019. Identification and functional characterisation of a novel N-cyanoamidine neonicotinoid metabolising cytochrome P450, CYP9Q6, from the buff-tailed bumblebee Bombus terrestris. Insect Biochemistry and Molecular Biology. 111 (103171). https://doi.org/10.1016/j.ibmb.2019.05.006
Authors | Troczka, B. J., Homem, R. A., Reid, R., Beadle, K., Kohler, M, Zaworra, M, Field, L. M., Williamson, M. S., Nauen, R., Bass, C. and Davies, T. G. E. |
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Abstract | Recent work has shown that two bumblebee (Bombus terrestris) cytochrome P450s of the CYP9Q subfamily, CYP9Q4 and CYP9Q5, are important biochemical determinants of sensitivity to neonicotinoid insecticides. Here, we report the characterisation of a third P450 gene CYP9Q6, previously mis-annotated in the genome of B. terrestris, encoding an enzyme that metabolises the N-cyanoamidine neonicotinoids thiacloprid and acetamiprid with high efficiency. The genomic location and complete ORF of CYP9Q6 was corroborated by PCR and its metabolic activity characterised in vitro by expression in an insect cell line. CYP9Q6 metabolises both thiacloprid and acetamiprid more rapidly than the previously reported CYP9Q4 and CYP9Q5. We further demonstrate a direct, in vivo correlation between the expression of the CYP9Q6 enzyme in transgenic Drosophila melanogaster and an increased tolerance to thiacloprid and acetamiprid. We conclude that CYP9Q6 is an efficient metaboliser of N-cyanoamidine neonicotinoids and likely plays a key role in the high tolerance of B. terrestris to these insecticides. |
Keywords | Bumblebee; Neonicotinoids; Cytochrome P450; Mrtabolism |
Year of Publication | 2019 |
Journal | Insect Biochemistry and Molecular Biology |
Journal citation | 111 (103171) |
Digital Object Identifier (DOI) | https://doi.org/10.1016/j.ibmb.2019.05.006 |
Web address (URL) | https://www.sciencedirect.com/science/article/pii/S0965174819301067?via%3Dihub |
Open access | Published as ‘gold’ (paid) open access |
Funder project or code | Bayer Crop Science |
European Union’s Horizon 2020 research and innovation programme | |
BBSRC [Check] | |
BBSRC Strategic Programme in Smart Crop Protection | |
Publisher's version | |
Output status | E-publication ahead of print |
Copyright license | CC BY |
ISSN | 0965-1748 |
Publisher | Elsevier |
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