Identification of novel aphid-killing bacteria to protect plants.

A - Papers appearing in refereed journals

Paliwal, D., Hamilton, A. J., Barrett, G. A., Alberti, F., Emden, H. V., Monteil, C. L., Mauchline, T. H., Nauen, R., Wagstaff, C., Bass, C. G. and Jackson, R. W. 2022. Identification of novel aphid-killing bacteria to protect plants. Microbial Biotechnology. 15 (4), pp. 1203-1220. https://doi.org/10.1111/1751-7915.13902

AuthorsPaliwal, D., Hamilton, A. J., Barrett, G. A., Alberti, F., Emden, H. V., Monteil, C. L., Mauchline, T. H., Nauen, R., Wagstaff, C., Bass, C. G. and Jackson, R. W.
Abstract

Aphids, including the peach-potato aphid, Myzus persicae, are major insect pests of agriculture and horticulture, and aphid control measures are limited. There is therefore an urgent need to develop alternative and more sustainable means of control. Recent studies have shown that environmental microbes have varying abilities to kill insects. We screened a range of environmental bacteria isolates for their abilities to kill target aphid species. Tests demonstrated the killing aptitude of these bacteria against six aphid genera (including Myzus persicae). No single bacterial strain was identified that was consistently toxic to insecticide-resistant aphid clones than susceptible clones, suggesting resistance to chemicals is not strongly correlated with bacterial challenge. Pseudomonas fluorescens PpR24 proved the most toxic to almost all aphid clones whilst exhibiting the ability to survive for over three weeks on three plant species at populations of 5–6 log CFU cm−2 leaf. Application of PpR24 to plants immediately prior to introducing aphids onto the plants led to a 68%, 57% and 69% reduction in aphid populations, after 21 days, on Capsicum annuum, Arabidopsis thaliana and Beta vulgaris respectively. Together, these findings provide new insights into aphid susceptibility to bacterial infection with the aim of utilizing bacteria as effective biocontrol agents.

Year of Publication2022
JournalMicrobial Biotechnology
Journal citation15 (4), pp. 1203-1220
Digital Object Identifier (DOI)https://doi.org/10.1111/1751-7915.13902
Open accessPublished as ‘gold’ (paid) open access
Publisher's version
Output statusPublished
Publication dates
Online01 Aug 2021
Publication process dates
Accepted16 Jul 2021
ISSN1751-7915
PublisherWiley

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