Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat

A - Papers appearing in refereed journals

Kavamura, V. N., Robinson, R. J., Hayat, R., Clark, I. M., Hughes, D. J., Rossmann, M., Hirsch, P. R., Mendes, R. and Mauchline, T. H. 2019. Land management and microbial seed load effect on rhizosphere and endosphere bacterial community assembly in wheat. Frontiers in Microbiology. 10 (2625).

AuthorsKavamura, V. N., Robinson, R. J., Hayat, R., Clark, I. M., Hughes, D. J., Rossmann, M., Hirsch, P. R., Mendes, R. and Mauchline, T. H.
Abstract

Microbial community ecology studies have traditionally utilized culture-based methodologies, though the advent of next-generation amplicon sequencing has facilitated superior resolution analyses of complex microbial communities. Here, we used culture-dependent and -independent approaches to explore the influence of land use as well as microbial seed load on bacterial community structure of the wheat rhizosphere and root endosphere. It was found that niche was an important factor in shaping the microbiome when using both methodological approaches, and that land use was also
a discriminatory factor for the culture-independent-based method. Although cultureindependent methods provide a higher resolution of analysis, it was found that in the
rhizosphere, particular operational taxonomic units (OTUs) in the culture-dependent fraction were absent from the culture-independent fraction, indicating that deeper sequence analysis is required for this approach to be exhaustive. We also found that the microbial seed load defined the endosphere, but not rhizosphere, community structure for plants grown in soil which was not wheat adapted. Together, these findings increase our understanding of the importance of land management and microbial seed load in shaping the root microbiome of wheat and this knowledge will facilitate the exploitation of plant–microbe interactions for the development of novel microbial inoculants.

KeywordsWheat; Microbiome; Rhizosphere; Endosphere; Seed; Embryo
Year of Publication2019
JournalFrontiers in Microbiology
Journal citation10 (2625)
Digital Object Identifier (DOI)doi:10.3389/fmicb.2019.02625
Web address (URL)https://www.frontiersin.org/article/10.3389/fmicb.2019.02625
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeBilateral BBSRC - Embrapa. Exploitation of the rhizosphere microbiome for sustainable wheat production
Publisher's version
Accepted author manuscript
Output statusPublished
Publication dates
Online15 Nov 2019
Publication process dates
Accepted29 Oct 2019
PublisherFrontiers Media SA
ISSN1664-302X

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