Transcriptome changes induced by arbuscular mycorrhizal fungi in sunflower (Helianthus annuus L.) roots

A - Papers appearing in refereed journals

Vangelisti, A., Natali, L., Bernardi, R., Sbrana, C., Turrini, A., Hassani-Pak, K., Hughes, D. J., Cavallini, A., Giovannetti, M. and Giordani, T. 2018. Transcriptome changes induced by arbuscular mycorrhizal fungi in sunflower (Helianthus annuus L.) roots. Scientific Reports. 8 (4), pp. 1-14. https://doi.org/10.1038/s41598-017-18445-0

AuthorsVangelisti, A., Natali, L., Bernardi, R., Sbrana, C., Turrini, A., Hassani-Pak, K., Hughes, D. J., Cavallini, A., Giovannetti, M. and Giordani, T.
Abstract

Arbuscular mycorrhizal (AM) fungi are essential elements of soil fertility, plant nutrition and productivity, facilitating soil mineral nutrient uptake. Helianthus annuus is a non-model, widely cultivated species. Here we used an RNA-seq approach for evaluating gene expression variation at early and late stages of mycorrhizal establishment in sunflower roots colonized by the arbuscular fungus Rhizoglomus irregulare. mRNA was isolated from roots of plantlets at 4 and 16 days after inoculation with the fungus. cDNA libraries were built and sequenced with Illumina technology. Differential expression analysis was performed between control and inoculated plants. Overall 726 differentially expressed genes (DEGs) between inoculated and control plants were retrieved. The number of upregulated DEGs greatly exceeded the number of down-regulated DEGs and this difference increased in later stages of colonization. Several DEGs were specifically involved in known mycorrhizal processes, such as membrane transport, cell wall shaping, and other. We also found previously unidentified mycorrhizal-induced transcripts. The most important DEGs were carefully described in order to hypothesize their roles in AM symbiosis. Our data add a valuable contribution for deciphering biological processes related to beneficial fungi and plant symbiosis, adding an Asteraceae, non-model species for future comparative functional genomics studies.

Keywordsmicrobiology, arbuscular mycorrhiza, Rhizoglomus irregulare, RNA-Seq, differential expression
Year of Publication2018
JournalScientific Reports
Journal citation8 (4), pp. 1-14
Digital Object Identifier (DOI)https://doi.org/10.1038/s41598-017-18445-0
Open accessPublished as ‘gold’ (paid) open access
Publisher's version
Output statusPublished
Publication dates
Print08 Jan 2018
Publication process dates
Accepted08 Dec 2017
PublisherNature Publishing Group
ISSN2045-2322

Permalink - https://repository.rothamsted.ac.uk/item/8464x/transcriptome-changes-induced-by-arbuscular-mycorrhizal-fungi-in-sunflower-helianthus-annuus-l-roots

29 total views
56 total downloads
2 views this month
0 downloads this month
Download files as zip