Detection of the nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR

A - Papers appearing in refereed journals

Hirsch, P. R., Mauchline, T. H., Mendum, T. A. and Kerry, B. R. 2000. Detection of the nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR. Mycological Research. 104 (4), pp. 435-439.

AuthorsHirsch, P. R., Mauchline, T. H., Mendum, T. A. and Kerry, B. R.
Abstract

PCR-based methods to detect Verticillium chlamydosporium on infected plant roots were developed. Arbitrary ERIC primers and those based on rRNA genes, to identify fungi grown in pure culture, were unsuitable for DNA extracted from nematode-infested roots, because of interference by plant and nematode DNA. A novel method utilizing specific primers designed from an amplified and cloned fragment of the V. chlamydosporium β-tubulin gene was developed. Although it could not discriminate between different isolates of V. chlamydosporium, one primer set could identify the fungus on tomato roots infested with root-knot nematodes. The V. chlamydosporium β-tubulin sequence data showed close homology to sequences from plant endophytic Acremonium and Epichloe species and the saprotrophic Trichoderma viride. 

Year of Publication2000
JournalMycological Research
Journal citation104 (4), pp. 435-439
Digital Object Identifier (DOI)doi:10.1017/S0953756299001483
Open accessPublished as non-open access
Funder project or code321
210
435
PublisherElsevier

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